Genetic characterization of Moniliophthora perniciosa from Ecuador and in vitro sensitivity to compost tea

2019 ◽  
Vol 154 (4) ◽  
pp. 943-959
Author(s):  
María Gabriela Maridueña-Zavala ◽  
Andrea Freire-Peñaherrera ◽  
Rodrigo Fernando Espinoza-Lozano ◽  
Mirian Villavicencio-Vasquez ◽  
Maria Jimenez-Feijoo ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Compost Tea ◽  
Moniliophthora Perniciosa ◽  
In Vitro Sensitivity

TEMPORAL GENETIC CHARACTERIZATION OF ISOLATES OF Moniliophthora perniciosa IN SOUTHEAST OF BAHIA, BRAZIL

2012 ◽  
Vol 24 (3) ◽  
pp. 169-178 ◽  
Author(s):  
Nara Georgia Ribeiro Braz Patrocinio ◽  
Ricardo Franco C. Moreira ◽  
Walnize de Oliveira Serra ◽  
José Luis Pires ◽  
Karina Peres Gramacho
Keyword(s):  
Genetic Characterization ◽  
Moniliophthora Perniciosa

Genetic characterization of the multipotent dedifferentiated state of pigmented epithelial cells in vitro

Development ◽  
1993 ◽  
Vol 118 (4) ◽  
pp. 1025-1030
Author(s):  
K. Agata ◽  
H. Kobayashi ◽  
Y. Itoh ◽  
M. Mochii ◽  
K. Sawada ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Genetic Characterization ◽  
Pigment Cells ◽  
Intermediate Cell ◽  
Lens Cells ◽  
Myc Gene ◽  
Retinal Pigmented Epithelial Cells ◽  
Testicular Hyaluronidase

Retinal pigmented epithelial cells (PECs) of chicken embryos extensively and almost synchronously transdifferentiate into lens cells in medium containing phenylthiourea and testicular hyaluronidase, passing through the bipotent dedifferentiated state. We have isolated genes that are expressed specifically by either pigment or lens cells and analyzed their expression in the transdifferentiation process. The expression of some proto-oncogenes was also studied. In the dedifferentiation process, expression of the c-myc gene was enhanced and the transcription of PEC-specific genes (MMP115, pP344) was completely repressed. However, transcription of lens-specific genes (alpha-, beta- and delta-crystallins genes) remained silent in dedifferentiated pigment cells. Activation of len- or PEC-specific genes occurred only in conditions permissive for lens or PEC differentiation, respectively. These results indicated that lens transdifferentiation from PECs proceeds through a multipotent (or at least bipotent) intermediate cell state in which the c-myc gene is activated, but neither PEC- nor lens-specific genes are expressed.

scholarly journals Physical and Genetic Characterization of Deletion Mutants of Simian Virus 40 Constructed In Vitro

1977 ◽  
Vol 24 (1) ◽  
pp. 277-294 ◽  
Author(s):  
Charles N. Cole ◽  
Terry Landers ◽  
Stephen P. Goff ◽  
Simone Manteuil-Brutlag ◽  
Paul Berg
Keyword(s):  
Genetic Characterization ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Deletion Mutants

The first isolation, in vitro propagation, and genetic characterization of Ehrlichia canis in Israel

1996 ◽  
Vol 62 (3-4) ◽  
pp. 331-340 ◽  
Author(s):  
Avi Keysary ◽  
Trevor Waner ◽  
Miri Rosner ◽  
Cynthia K. Warner ◽  
Jacqueline E. Dawson ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Genetic Characterization ◽  
Ehrlichia Canis

Isolation and genetic characterization of Neospora caninum from asymptomatic calves in Spain

Parasitology ◽  
2008 ◽  
Vol 135 (14) ◽  
pp. 1651-1659 ◽  
Author(s):  
J. REGIDOR-CERRILLO ◽  
M. GÓMEZ-BAUTISTA ◽  
J. PEREIRA-BUENO ◽  
G. ADURIZ ◽  
V. NAVARRO-LOZANO ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Characterization ◽  
Neospora Caninum ◽  
Epidemiological Features ◽  
Newborn Calves ◽  
Genetic Profiles ◽  
The Relationship ◽  
Infected Newborn

SUMMARYNeospora caninum is a cyst-forming parasite that causes abortion in cattle. Despite this parasite's ubiquitous distribution and wide host range, the number of N. caninum isolates obtained to date is limited. In vitro isolation of the parasite is arduous and often unsuccessful. In addition, most isolates have been obtained from clinically affected hosts and therefore could be biased towards more virulent isolates. In this report, an improved isolation approach from transplacentally infected newborn calves was undertaken and 9 new isolates were obtained. Moreover, a microsatellite technique was applied to investigate the genetic diversity of these isolates. Most isolates showed specific genetic profiles. However, the Nc-Spain10 isolate was identical to the previously described Nc-Spain1H isolate and Nc-Spain3H was identical to Nc-Spain4H. These isolates were likely to have identical genotypes because they were isolated from distinct calves of the same herd. Future pathogenic characterization of these isolates will contribute to the investigation of the relationship between isolate virulence and the outcome of infection, as well as other epidemiological features, such as transmission.

scholarly journals Pathogenicity of Moniliophthora roreri isolates from selected morphology groups in harvested cacao pods and in vitro sensitivity to compost tea.

Bionatura ◽  
2021 ◽  
Vol 6 (1) ◽  
pp. 1569-1574
Author(s):  
Maria Isabel Jimenez Feijoo ◽  
Juan Manuel Cevallos-Cevallos
Keyword(s):  
High Sensitivity ◽  
Severity Index ◽  
Potato Dextrose Agar ◽  
Compost Tea ◽  
In Vitro Sensitivity ◽  
Progress Curve ◽  
Rot Disease ◽  
Moniliophthora Roreri ◽  
Frosty Pod Rot

Moniliopthora roreri is the frosty pod rot disease (FPD) and one of the most devastating cacao pathogens worldwide. However, M. roreri pathogenicity on harvested cacao pods and sensitivity to compost tea have not been fully described. Monosporic cultures of M. roreri from different morphology groups were obtained. The isolates’ pathogenicity was tested by inoculation onto harvested cacao pods, and symptoms were evaluated at 3-day intervals during 16 days before estimating the area under the disease progress curve (AUDPC). The sensitivity of M. roreri to compost tea was evaluated on potato dextrose agar (PDA) amended with 1 to 5 % compost tea. All morphology groups could infect harvested cacao pods during the 16 days with a disease severity index abode 75 %. Compost tea completely inhibited the growth of M. roreri when used at 4.5 % or higher. Results suggest a shortened biotrophic phase during the infection in harvested pods and a medium to high sensitivity of M. roreri to compost tea.

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