scholarly journals Effect of Different Cytokinins and Media Types on In vitro Shoot Proliferation of Asparagus racemosus Willd.

2016 ◽  
Vol 26 (2) ◽  
pp. 151-157
Author(s):  
Shubha Thakur ◽  
KL Tiwari ◽  
SK Jadhav

The evaluation of the effects of different cytokinin concentrations and media types on in vitro shoot proliferation of Asparagus racemosus Willd. is reported. Maximum shoot number and shoot lengths were found with 0.25 mg/l BA which was statistically similar with 0.1 mg/l Kn. Maximum multiplication and growth were found in MS. The protocol could thus be helpful for in vitro mass propagation of A. racemosus.Plant Tissue Cult. & Biotech. 26(2): 151-157, 2016 (December)

2014 ◽  
Vol 23 (2) ◽  
pp. 283-288
Author(s):  
Veenu Joshi ◽  
S. K. Jadhav

D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17529Plant Tissue Cult. & Biotech. 23(2): 283-288, 2013  (December)


1970 ◽  
Vol 34 (3) ◽  
pp. 411-416 ◽  
Author(s):  
MS Parvin ◽  
ME Haque ◽  
F Akhter ◽  
M Moniruzzaman ◽  
ABM Khaldun

The present study was conducted to investigate the effect of growth regulator NAA on in vitro shoot proliferation, rooting, and plantlet establishment. Among the different concentrations of NAA, the best increase in shoot weight (0.25 g) and shoot number (8.83) were observed from 0.1 mg/I NAA. The highest shoot length (2.60 cm), number of leaves (4.83), number of roots (5.15), and root length (2.67 cm) were obtained with 0.2 mg/I NAA at 60 DAT. Key Words: Dendrobium orchid, NAA, MS media. DOI: 10.3329/bjar.v34i3.3966 Bangladesh J. Agril. Res. 34(3) : 411-416, September 2009


HortScience ◽  
2005 ◽  
Vol 40 (1) ◽  
pp. 185-188 ◽  
Author(s):  
Samir C. Debnath

In an attempt to improve the micropropagation protocol for lingonberry (Vaccinium vitis-idaea L.) developed at the Centre, two lingonberry clones were compared for in vitro shoot proliferation on two different media supplemented with varying levels of thidiazuron (TDZ). TDZ supported proliferation at low concentrations (0.1 to 1 μm) but inhibited shoot elongation. However, usable shoots were obtained within 4 weeks by transferring shoot cluster to medium containing 1 μm zeatin. Genotypes differed significantly with respect to multiplication rate with `EL1' producing the most shoots per explant. In both genotypes, shoot proliferation was greatly influenced by explant orientation. Changing the orientation of explants from vertically upright to horizontal increased axillary shoot number, but decreased shoot height and leaf number per shoot. Proliferated shoots were rooted on a 2 peat: 1 perlite (v/v) medium, and the plantlets were acclimatized and eventually established in the greenhouse.


3 Biotech ◽  
2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Mouaad Amine Mazri ◽  
Reda Meziani ◽  
Jamal El Fadile ◽  
Az-eddine Ezzinbi

2011 ◽  
Vol 21 (2) ◽  
pp. 135-141 ◽  
Author(s):  
A.K. M. Sayeed Hassan ◽  
Nadira Begum ◽  
Rebeka Sultana ◽  
Rahima Khatun

An efficient protocol was developed for shoot proliferation and plant regeneration of Phlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA + 0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration   D. O. I. 10.3329/ptcb.v21i2.10236   Plant Tissue Cult. & Biotech. 21(2): 135-141, 2011 (December)


1989 ◽  
Vol 58 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Hideki MURAYAMA ◽  
Ryutaro TAO ◽  
Tatsumi TANAKA ◽  
Akira SUGIURA

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