Genetic resistance to fusariosis and base rot in interspecific F1 progenies of passion fruit (Passiflora spp.)

Fusariosis and base rot, caused by the fungi Fusarium oxysporum f. sp. passiflorae and F. solani, respectively, seriously damage the cultivation of sour passion fruit in Brazil. This work aims to obtain and evaluate F1 hybrids of Passiflora spp. wild plants resistant to fusariosis and base rot using genotypes of P. edulis (commercial species) and certify interspecific hybridizations using microsatellite markers. Hybridizations were performed using a P. edulis female parent and P. nitida and P. mucronata male parents for crosses aiming fusariosis resistance, and P. nitida, P. cincinnata and P. quadrangularis for hybridizations aiming tolerance to base rot. 35 microsatellite markers were used to confirm hybridization. The washed roots method was used for fusarium resistance tests and inoculation procedure with a mycelium disk fixed on a small wound on the plant stem for base rot. The interspecific hybridizations provided 49 potentially hybrid genotypes. Confirmation of hybridization by microsatellite marker was verified for 57% of the analyzed genotypes. The hybrids 115-1, 115-3, 115-4, 115-5, 115-6, 115-7, 115-9 and 128 are indicated as promising genotypes for a new stage of the breeding program. In the resistance evaluation of 13 F1 hybrids to F. oxysporum f. sp. passiflorae, the genotypes 142 and 143-2 were selected as the most resistant to continue the breeding program.

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Generation of interspecific hybrids for introgression of mungbean yellow mosaic virus resistance in Vigna radiata (L.) Wilczek

Legume Research - An International Journal ◽

10.18805/lr-3808 ◽

2018 ◽

Cited By ~ 3

Author(s):

Prince Lekhi ◽

R. K. Gill ◽

Satinder Kaur ◽

T. S. Bains

Keyword(s):

Microsatellite Markers ◽

Mosaic Virus ◽

Vigna Radiata ◽

Female Parent ◽

Germination Percentage ◽

Yellow Mosaic Virus ◽

F1 Hybrids ◽

Polymorphic Markers ◽

Vigna Species ◽

Mungbean Yellow Mosaic Virus

Vigna radiata genotypes viz., SML 668 and SML 832 and V. mungo genotypes viz., Mash 114 and Mash 218 were crossed in all possible combinations during summer 2015 to generate F1 hybrids. Interspecific hybridization was attempted by using V. radiata genotypes as female parent. Pod set percentage varied from 5.5 percent (SML 832 x Mash 218) to 24.1 percent (SML 832 x Mash 114). The germination percentage ranged from 14.29 to 30.56. Maximum pollen fertility was observed in cross SML 668 x Mash 114 (28.36 percent) followed by SML 668 x Mash 218 (27.03 percent), SML 832 x Mash 218 (24.32 percent) and minimum in SML 832 x Mash 114 (22.59 percent).The purity of hybrids were tested through microsatellite markers. For parental polymorphism, microsatellite markers were selected from related Vigna species such as Vigna unguiculata, Vigna radiata and Vigna mungo. Out of 84 markers used, 46 were polymorphic i.e 54.76 per cent polymorphism between parents. These polymorphic markers were used for confirmation of hybrids produced from different crosses. All the F1 plants gave resistant reaction to Mungbean yellow mosaic virus (MYMV) indicating the introgression of resistance gene(s) from V. mungo to V. radiata.

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Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2011001100009 ◽

2011 ◽

Vol 46 (11) ◽

pp. 1480-1488 ◽

Cited By ~ 9

Author(s):

Mariane Ruzza Schuck ◽

Luiz Antonio Biasi ◽

Ada Michele Mariano ◽

Bernardo Lipski ◽

Summaira Riaz ◽

...

Keyword(s):

Microsatellite Markers ◽

Interspecific Hybridization ◽

Molecular Analysis ◽

Interspecific Hybrids ◽

Female Parent ◽

F1 Hybrids ◽

Reciprocal Crosses ◽

Self Pollination ◽

Vitis Labruscana ◽

Muscadinia Rotundifolia

The objective of this work was to assess the potential of interspecific hybridization of Vitis labruscana and Muscadinia rotundifolia by using artificial cross-pollinations. Microsatellite markers were used to confirm interspecific hybridizations and the identity of the parental genotypes. In crosses in which M. rotundifolia was used as the female parent, no true hybrids were obtained. In the reciprocal crosses, 114 seedlings were identified as true V. labruscana x M. rotundifolia hybrids. Self pollination occurred in direct and in reciprocal crosses. The crossings between 'Bordo' x 'Carlos', 'Magnolia', 'Regale' and' Roanoke', and between' Isabel' x 'Bountiful', 'Carlos', 'Magnolia', 'Regale' and 'Roanoke' were confirmed. The 15 markers evaluated showed that two M. rotundifolia parental genotypes had the same fingerprint profile, indicating a like lyplanting error. The success of hybridization depends mainly on the species and on the cultivar used as the female parent. Microsatellite markers are efficient to confirm the paternity of interspecific F1 hybrids and to determine the correct identity of M. rotundifolia cultivars.

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Isolation and characterization of microsatellite markers from the sweet passion fruit (Passiflora alata Curtis: Passifloraceae)

Molecular Ecology Notes ◽

10.1111/j.1471-8286.2005.01090.x ◽

2005 ◽

Vol 5 (4) ◽

pp. 863-865 ◽

Cited By ~ 24

Author(s):

J. G. PADUA ◽

E. J. OLIVEIRA ◽

M. I. ZUCCHI ◽

G. C. X. OLIVEIRA ◽

L. E. A. CAMARGO ◽

...

Keyword(s):

Microsatellite Markers ◽

Passion Fruit ◽

Isolation And Characterization

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Evaluation of powdery mildew resistance in various melon (Cucumis melo L.) genotypes

Agricultural Science and Technology ◽

10.15547/10.15547/ast.2018.04.053 ◽

2018 ◽

Vol 10 (4) ◽

pp. 279-284

Author(s):

Zh. Ivanova ◽

K. Vasileva ◽

N. Velkov ◽

S. Grozeva

Keyword(s):

Powdery Mildew ◽

Genetic Resistance ◽

Leaf Disc ◽

Breeding Program ◽

Vegetable Crops ◽

Plant Materials ◽

South Central ◽

Leaf Disc Assay ◽

Disc Assay

Abstract. Powdery mildew, caused by Podosphaera xanthii and Golovinomyces cichoracearum, is an economically important disease in melon worldwide. Genetic resistance is one of the most suitable strategies to control powdery mildew. During the last few years several races of the pathogens have been reported. The need to develop resistant varieties is a challenge for each breeding program. Leaf disc assay was used in phytopathology and breeding programs as a rapid and reliable method for evaluation of disease resistance in a large number of plant materials. The purpose of this study was to establish species and races of powdery mildew in Plovdiv region, South Central Bulgaria; to develop a suitable system of pathogen isolation and cultivation; to determine the resistance levels in different melon genotypes available in Maritsa Vegetable Crops Research Institute (MVCRI) - Plovdiv collection by the leaf disc assay. Fifty-three melon genotypes, including lines, varieties, hybrids and ten differential lines were tested. The data showed that causal agent of powdery mildew was race 1 of P. xanthii in Plovdiv region. Our experimental results indicated that for the long-term storage of powdery mildew it is preferable to keep a whole plant under in vitro conditions. This allows the preservation of powdery mildew for two months before transferring on a new tissue. Thirty-four of the tested melon genotypes reacted as immune or resistant and nineteen as susceptible. Resistant melon genotypes are a suitable source in initiating a new breeding program aimed to increase resistance to powdery mildew.

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Assessment of resistance of hybrids and lineages of passion fruit to bacteriosis under protected cultivation

Bioscience Journal ◽

10.14393/bj-v36n4a2020-48021 ◽

2020 ◽

Vol 36 (4) ◽

Author(s):

Janlylle Ruama Yankovich Arrifano ◽

José Ricardo Peixoto ◽

Michelle Souza Vilela ◽

Solange da Costa Nogueira ◽

Thiago Campos De Oliveira ◽

...

Keyword(s):

Genetic Resistance ◽

Passion Fruit ◽

Bacterial Spot ◽

Xanthomonas Axonopodis ◽

Affected Area ◽

Injured Area ◽

Protected Cultivation ◽

Diagrammatic Scale ◽

Yellow Passion Fruit ◽

Sour Passion Fruit

Yield and longevity of yellow passion fruit have been reduced by diseases such as the bacterial spot caused by Xanthomonas axonopodis pv. passiflorae. Genetic resistance has been confirmed as the most efficient and economical correct option to minimize this disease problem. Aiming at it, the objective of this research was to evaluate the incidence, severity and progression of the disease in 12 genotypes of sour passion fruit, in seedling stage in nursery greenhouse after inoculation of Xanthomonas axonopodis pv. passiflorae. The inoculation was performed with an isolate collected in the Pipiripau Rural Nucleus, Brasilia-DF, named UnB-1397 (1x106 CFU/mL), through induction of injuries. There were performed four assessments, with interval of 7 days except the first which was performed 11 days after inoculation. The incidence was estimated by the percentage of plants affected. To evaluate the severity, it was used the diagrammatic scale validated by Costa et al. (2018), with adaptations, using the measurement of the affected area by necrotic lesions on the leaf. All genotypes were susceptible to bacteriosis, 5 being considered moderately susceptible: F1 BRS Pérola do Cerrado x Rosa Intenso, Mar20#21, Mar20#15b, Mar20#24xMar20#40 and FB200PL4R2 x Mar20#2005, with a mean of severity ranging from 11 to 25% of injured area in leaves.

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Genome-wide development of polymorphic microsatellite markers and their application in peanut breeding program

Electronic Journal of Biotechnology ◽

10.1016/j.ejbt.2020.01.004 ◽

2020 ◽

Vol 44 ◽

pp. 25-32

Author(s):

Junjie Ma ◽

Yuhan Zhao ◽

Hua Chen ◽

Chun Fu ◽

Lin Zhu ◽

...

Keyword(s):

Microsatellite Markers ◽

Breeding Program ◽

Genome Wide ◽

Polymorphic Microsatellite

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Utility Assessment of Published Microsatellite Markers for Fiber Length and Bundle Strength QTL in a Cotton Breeding Program

Crop Science ◽

10.2135/cropsci2016.02.0093 ◽

2016 ◽

Vol 56 (6) ◽

pp. 2983-2995 ◽

Cited By ~ 4

Author(s):

Kari L. Hugie ◽

David D. Fang ◽

C. Wayne Smith ◽

Ping Li ◽

Lori L. Hinze ◽

...

Keyword(s):

Microsatellite Markers ◽

Fiber Length ◽

Breeding Program ◽

Utility Assessment ◽

Cotton Breeding

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Genomic Profiling of F1 Hybrids of Durian (Durio zibethinus) Revealed by RAPD-PCR

Journal of Horticultural Research ◽

10.1515/johr-2016-0022 ◽

2016 ◽

Vol 24 (2) ◽

pp. 69-76 ◽

Cited By ~ 1

Author(s):

Riry Prihatini ◽

Farihul Ihsan ◽

Ni Luh Putu Indriyani

Keyword(s):

Molecular Analysis ◽

Genetic Relationships ◽

Female Parent ◽

Similarity Coefficient ◽

F1 Hybrids ◽

Genomic Profiling ◽

Pca Analysis ◽

Genetic Characteristics ◽

Durio Zibethinus ◽

Rapd Pcr

Abstract The molecular analysis of 32 durian F1 hybrids, resulted from crossing of the Arp 8990 (female parent) and ‘Otong’ (male parent), was conducted in order to determine the genetic characteristics of hybrids and parents, as it would be followed/evidenced by the variability of traits produced from the cross breeding. The RAPD analyses of 14 primers resulted in 114 scoring bands, 112 (98.2%) of them were polymorphic, with 4 to 11 bands amplified per primer. The electrophoresis gel of the PCR results revealed that some hybrids produced different band patterns compared to the parents; this indicated the crossing between parents’ alleles and trait combinations from both the parents. The Dice-Sorensen similarity coefficient demonstrated that most of the hybrids had distant genetic similarities with both parents, which were ranged from 0.141 [71B(4) and 72B(15)] to 0.776 [71B(15) and 48B(1)]. The UPGMA method was used to construct the dendrogram, which grouped the hybrids in five clusters with distinct genetic relationships and was confirmed with the PCA analysis. This result implied that above crossing produced hybrids having characters different from the parents.

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