Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine

The objective of this work was to assess the potential of interspecific hybridization of Vitis labruscana and Muscadinia rotundifolia by using artificial cross-pollinations. Microsatellite markers were used to confirm interspecific hybridizations and the identity of the parental genotypes. In crosses in which M. rotundifolia was used as the female parent, no true hybrids were obtained. In the reciprocal crosses, 114 seedlings were identified as true V. labruscana x M. rotundifolia hybrids. Self pollination occurred in direct and in reciprocal crosses. The crossings between 'Bordo' x 'Carlos', 'Magnolia', 'Regale' and' Roanoke', and between' Isabel' x 'Bountiful', 'Carlos', 'Magnolia', 'Regale' and 'Roanoke' were confirmed. The 15 markers evaluated showed that two M. rotundifolia parental genotypes had the same fingerprint profile, indicating a like lyplanting error. The success of hybridization depends mainly on the species and on the cultivar used as the female parent. Microsatellite markers are efficient to confirm the paternity of interspecific F1 hybrids and to determine the correct identity of M. rotundifolia cultivars.

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Interspecific Hybrids between Tea (Camellia sinensis) and Wilson's Camellia (C. irrawadiensis) I. Morphology and Cytology of F1 Hybrids

Experimental Agriculture ◽

10.1017/s0014479700006177 ◽

1975 ◽

Vol 11 (1) ◽

pp. 13-16 ◽

Cited By ~ 4

Author(s):

H. P. Bezbaruah

Keyword(s):

Camellia Sinensis ◽

Pollen Fertility ◽

Interspecific Hybrids ◽

Female Parent ◽

F1 Hybrids ◽

High Pollen ◽

Reciprocal Crosses ◽

Close Relationship ◽

Anatomical Characters ◽

Regular Meiosis

SUMMARYCrosses were made between tea (Camellia sinensis L.) and Wilson's camellia (C. irrawadiensis Barua), using tea as the female parent, but reciprocal crosses were not successful. The F1 hybrids were intermediate in morphological and anatomical characters between the parents. Regular meiosis and high pollen fertility in the hybrids clearly indicate a close relationship between the parental genomes.

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Production and Molecular Identification of Interspecific Hybrids between Phaius mishmensis (Lindl. and Paxton) Rchb. f. and Phaius tankervilliae (Banks) Blume

Agriculture ◽

10.3390/agriculture11040306 ◽

2021 ◽

Vol 11 (4) ◽

pp. 306

Author(s):

Tasanai Punjansing ◽

Maliwan Nakkuntod ◽

Somjit Homchan ◽

Phithak Inthima ◽

Anupan Kongbangkerd

Keyword(s):

Interspecific Hybridization ◽

Interspecific Hybrids ◽

Parental Species ◽

Female Parent ◽

Genetic Fidelity ◽

Germination Percentage ◽

F1 Hybrids ◽

Scar Markers ◽

Sequence Characterized Amplified Region ◽

Decamer Primer

This study aimed at assessing the hybridization feasibility and evaluating genetic fidelity of the hybrid seedlings originated from Phaius mishmensis (Lindl. and Paxton) Rchb. f. and P. tankervilliae (Banks) Blume. Intra- and interspecific hybridization between Phaius mishmensis (Lindl. and Paxton) Rchb. f. and P. tankervilliae (Banks) Blume were examined to establish the primary hybrid, observe their cross ability and identify the F1 hybrids using sequence-characterized amplified region (SCAR) markers. Self-incompatibility and cross ability of P. mishmensis and P. tankervilliae were tested before starting the breeding program. Results showed that they were self-compatible orchids. The interspecific hybridization between P. mishmensis and P. tankervilliae was achieved with the highest pod setting (80%), seed germination percentage (94.8%) and the rate of protocorm development into mature seedlings (stage 6) (10.6%), but the smallest size of embryo with width 46.5 μm, length 67.3 μm was also observed when P. mishmensis was taken as the female parent. A comparative study on leaf morphology and anatomy of plantlets regenerated from intra- and interspecific hybrids of P. mishmensis and P. tankervilliae showed a transitional character to the parental species. Herein, the presence of interspecific hybrids between P. mishmensis and P. tankervilliae, as well as their reciprocal cross, was verified using Pmis524 SCAR markers developed by the decamer primer.

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Genomic Profiling of F1 Hybrids of Durian (Durio zibethinus) Revealed by RAPD-PCR

Journal of Horticultural Research ◽

10.1515/johr-2016-0022 ◽

2016 ◽

Vol 24 (2) ◽

pp. 69-76 ◽

Cited By ~ 1

Author(s):

Riry Prihatini ◽

Farihul Ihsan ◽

Ni Luh Putu Indriyani

Keyword(s):

Molecular Analysis ◽

Genetic Relationships ◽

Female Parent ◽

Similarity Coefficient ◽

F1 Hybrids ◽

Genomic Profiling ◽

Pca Analysis ◽

Genetic Characteristics ◽

Durio Zibethinus ◽

Rapd Pcr

Abstract The molecular analysis of 32 durian F1 hybrids, resulted from crossing of the Arp 8990 (female parent) and ‘Otong’ (male parent), was conducted in order to determine the genetic characteristics of hybrids and parents, as it would be followed/evidenced by the variability of traits produced from the cross breeding. The RAPD analyses of 14 primers resulted in 114 scoring bands, 112 (98.2%) of them were polymorphic, with 4 to 11 bands amplified per primer. The electrophoresis gel of the PCR results revealed that some hybrids produced different band patterns compared to the parents; this indicated the crossing between parents’ alleles and trait combinations from both the parents. The Dice-Sorensen similarity coefficient demonstrated that most of the hybrids had distant genetic similarities with both parents, which were ranged from 0.141 [71B(4) and 72B(15)] to 0.776 [71B(15) and 48B(1)]. The UPGMA method was used to construct the dendrogram, which grouped the hybrids in five clusters with distinct genetic relationships and was confirmed with the PCA analysis. This result implied that above crossing produced hybrids having characters different from the parents.

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Generation of interspecific hybrids for introgression of mungbean yellow mosaic virus resistance in Vigna radiata (L.) Wilczek

Legume Research - An International Journal ◽

10.18805/lr-3808 ◽

2018 ◽

Cited By ~ 3

Author(s):

Prince Lekhi ◽

R. K. Gill ◽

Satinder Kaur ◽

T. S. Bains

Keyword(s):

Microsatellite Markers ◽

Mosaic Virus ◽

Vigna Radiata ◽

Female Parent ◽

Germination Percentage ◽

Yellow Mosaic Virus ◽

F1 Hybrids ◽

Polymorphic Markers ◽

Vigna Species ◽

Mungbean Yellow Mosaic Virus

Vigna radiata genotypes viz., SML 668 and SML 832 and V. mungo genotypes viz., Mash 114 and Mash 218 were crossed in all possible combinations during summer 2015 to generate F1 hybrids. Interspecific hybridization was attempted by using V. radiata genotypes as female parent. Pod set percentage varied from 5.5 percent (SML 832 x Mash 218) to 24.1 percent (SML 832 x Mash 114). The germination percentage ranged from 14.29 to 30.56. Maximum pollen fertility was observed in cross SML 668 x Mash 114 (28.36 percent) followed by SML 668 x Mash 218 (27.03 percent), SML 832 x Mash 218 (24.32 percent) and minimum in SML 832 x Mash 114 (22.59 percent).The purity of hybrids were tested through microsatellite markers. For parental polymorphism, microsatellite markers were selected from related Vigna species such as Vigna unguiculata, Vigna radiata and Vigna mungo. Out of 84 markers used, 46 were polymorphic i.e 54.76 per cent polymorphism between parents. These polymorphic markers were used for confirmation of hybrids produced from different crosses. All the F1 plants gave resistant reaction to Mungbean yellow mosaic virus (MYMV) indicating the introgression of resistance gene(s) from V. mungo to V. radiata.

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Outcrossing in interspecific hybrids between Eucalyptus spathulata and E. platypus

Australian Journal of Botany ◽

10.1071/bt04081 ◽

2005 ◽

Vol 53 (4) ◽

pp. 347 ◽

Cited By ~ 3

Author(s):

M. A. B. Wallwork ◽

M. Sedgley

Keyword(s):

Interspecific Hybrids ◽

Female Parent ◽

Pollen Tubes ◽

The Other ◽

Parent Species ◽

Cross Pollination ◽

Seed Maturity ◽

Self Pollination ◽

Ovule Penetration ◽

Zygotic Control

Outcrossing was investigated in interspecific hybrids between self-fertile Eucalyptus platypus Blakely and partially self-sterile E. spathulata Hook., which shows both pre- and post-zygotic timing. Four hybrid trees were used for the study, two with E. spathulata and two with E. platypus as female parent. Each hybrid had a similar number of locules to each other and to the E. platypus parent, and an intermediate number of ovules per flower compared with the parent species. Controlled hand-pollinations were carried out, in which both self- and cross-pollen from the other hybrid tree with the same female parent species was applied to flowers on each of the four trees, and observations were made 10days, 4weeks and 8weeks after pollination and at seed maturity. In all hybrids, mean seeds per capsule was consistently higher following cross-pollination than following self-pollination. All hybrids showed a reduction in pollen tube number between the top and base of the style when examined by fluorescence microscopy. One tree had significantly fewer cross- than self-pollen tubes at the base of the style, but a similar number of ovules was penetrated by pollen tubes following both treatments. In the other three, there was no difference between cross- and self-pollination in pollen tubes in the style. In three of the four trees there was no difference in ovule penetration following self- or cross-pollination, but in the other, more crossed than selfed ovules were penetrated. Light-microscopy observation of ovules indicated that ovule abortion following fertilisation accounted for the reduced numbers of seeds following self-pollination and to a lesser extent following cross-pollination. All four hybrid trees, irrespective of female parent, were partially self-sterile and resembled the partially self-sterile E. spathulata rather than the self-fertile E. platypus. While the timing of outcrossing control of E. spathulata was both pre- and post-zygotic, only one hybrid was similar, with the other three showing post-zygotic control.

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Effect of Meiotic Polyploidisation on Selected Morphological and Anatomical Traits in Interspecific Hybrids of Brassica oleracea × B. napus

Agronomy ◽

10.3390/agronomy12010026 ◽

2021 ◽

Vol 12 (1) ◽

pp. 26

Author(s):

Agnieszka Marasek-Ciolakowska ◽

Piotr Kamiński ◽

Małgorzata Podwyszyńska ◽

Urszula Kowalska ◽

Michał Starzycki ◽

...

Keyword(s):

Interspecific Hybrids ◽

Pollen Viability ◽

Inbred Lines ◽

F1 Hybrids ◽

Interspecific Hybridisation ◽

Self Pollination ◽

F2 Hybrids ◽

F2 Generation ◽

Head Cabbage ◽

F1 Generation

In Brassica, interspecific hybridisation plays an important role in the formation of allopolyploid cultivars. In this study, the ploidy of F1 and F2 generations resulting from interspecific hybridisation between B. oleracea inbred lines of head cabbage (B. oleracea L. var. capitata) (2n = 18) and kale (B. oleracea L. var. acephala) (2n = 18) with inbred lines of rapeseed (B. napus L.) (2n = 38) was examined by flow cytometry analysis and chromosome observation. Furthermore, the effect of meiotic polyploidisation on selected phenotypic and anatomical traits was assessed. The F1 hybrids of head cabbage × rapeseed (S3) and kale × rapeseed crosses (S20) were allotriploids with 2n = 28 chromosomes, and nuclear DNA amounts of 1.97 (S3) and 1.99 pg (S20). These values were intermediate between B. oleracea and B. napus. In interspecific hybrids of the F2 generation, which were derived after self-pollination of F1 hybrids (FS3, FS20) or by open crosses between F1 generation hybrids (FC320, FC230), the chromosome numbers were similar 2n = 56 or 2n = 55, whereas the genome sizes varied between 3.81 (FS20) and 3.95 pg 2C (FC230). Allohexaploid F2 hybrids had many superior agronomic traits compared to parental B. napus and B. oleracea lines and triploid F1 hybrids. In the generative stage, they were characterised by larger flowers and flower elements, such as anthers and lateral nectaries. F2 hybrids were male and female fertile. The pollen viability of F2 hybrids was comparable to parental genotypes and varied from 75.38% (FS3) to 88.24% (FC320), whereas in triploids of F1 hybrids only 6.76% (S3) and 13.46% (S20) of pollen grains were fertile. Interspecific hybrids of the F2 generation derived by open crosses between plants of the F1 generation (FC320, FC230) had a better ability to set seed than F2 hybrids generated from the self-pollination of F1 hybrids. In the vegetative stage, F2 plants had bigger and thicker leaves, larger stomata, and significantly thicker layers of palisade and spongy mesophyll than triploids of the F1 generation and parental lines of B. oleracea and B. napus. The allohexaploid F2 hybrids analysed in this study can be used as innovative germplasm resources for further breeding new vegetable Brassica crops at the hexaploid level.

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Study of crossability and F1 of interspecific hybridization between Brassica rapa (B. campestris) and B. nigra

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v32i3.546 ◽

2008 ◽

Vol 32 (3) ◽

pp. 445-449

Author(s):

MA Malik

Keyword(s):

Chromosome Number ◽

Interspecific Hybridization ◽

Female Parent ◽

Pollen Sterility ◽

F1 Hybrids ◽

Root Tip ◽

Intermediate Morphology ◽

Pale Colour ◽

Tip Cells ◽

Root Tip Cells

Interspecific hybridization between yellow seeded variety, Binasarisha-6 of B. rapa var. Yellow Sarson (2n=20; AA) and Nigra-1 of B. nigra (2n=16; BB) were made. The crosses with Binasarisha-6 of B. rapa var. Yellow Sarson as a female parent were only successful. Chromosome number in root tip cells of the F1 hybrids was 18, which was half of the sum total of the somatic chromosome number of the parents and indicated hybrid nature. Hybrids exhibited intermediate morphology between the parents. All the hybrids showed complete pollen sterility with shrivelled, pointed tip, and pale colour anthers and reduced filaments and failed to set siliquae and seeds.DOI: http://dx.doi.org/10.3329/bjar.v32i3.546Bangladesh J. Agril. Res. 32(3) : 445-449, September 2007

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Genetic resistance to fusariosis and base rot in interspecific F1 progenies of passion fruit (Passiflora spp.)

Research Society and Development ◽

10.33448/rsd-v9i11.10519 ◽

2020 ◽

Vol 9 (11) ◽

pp. e96091110519

Author(s):

Thalita Neves Marostega ◽

Sandra da Costa Preisigke ◽

Antonio Marcos Chimello ◽

Thiago Alexandre Santana Gilio ◽

Kelly Lana Araujo ◽

...

Keyword(s):

Microsatellite Markers ◽

Genetic Resistance ◽

Female Parent ◽

Breeding Program ◽

Passion Fruit ◽

Wild Plants ◽

F1 Hybrids ◽

Commercial Species ◽

Inoculation Procedure ◽

Plant Stem

Fusariosis and base rot, caused by the fungi Fusarium oxysporum f. sp. passiflorae and F. solani, respectively, seriously damage the cultivation of sour passion fruit in Brazil. This work aims to obtain and evaluate F1 hybrids of Passiflora spp. wild plants resistant to fusariosis and base rot using genotypes of P. edulis (commercial species) and certify interspecific hybridizations using microsatellite markers. Hybridizations were performed using a P. edulis female parent and P. nitida and P. mucronata male parents for crosses aiming fusariosis resistance, and P. nitida, P. cincinnata and P. quadrangularis for hybridizations aiming tolerance to base rot. 35 microsatellite markers were used to confirm hybridization. The washed roots method was used for fusarium resistance tests and inoculation procedure with a mycelium disk fixed on a small wound on the plant stem for base rot. The interspecific hybridizations provided 49 potentially hybrid genotypes. Confirmation of hybridization by microsatellite marker was verified for 57% of the analyzed genotypes. The hybrids 115-1, 115-3, 115-4, 115-5, 115-6, 115-7, 115-9 and 128 are indicated as promising genotypes for a new stage of the breeding program. In the resistance evaluation of 13 F1 hybrids to F. oxysporum f. sp. passiflorae, the genotypes 142 and 143-2 were selected as the most resistant to continue the breeding program.

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Hybridization and Self-compatibility in Celtis: AFLP Analysis of Controlled Crosses

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.132.3.368 ◽

2007 ◽

Vol 132 (3) ◽

pp. 368-373 ◽

Cited By ~ 4

Author(s):

Alan T. Whittemore ◽

Alden M. Townsend

Keyword(s):

Dna Methylation ◽

Interspecific Hybridization ◽

Interspecific Hybrids ◽

Low Frequency ◽

Aflp Analysis ◽

Very Low Frequency ◽

Self Pollination ◽

Large Numbers ◽

Self Compatibility ◽

Controlled Crosses

Artificial cross-pollinations were carried out among seven species of Celtis L. (C. bungeana Blume, C. koraiensis Nakai, C. laevigata Willd., C. occidentalis L., C. reticulata Torr., C. sinensis Pers., and C. tenuifolia Nutt.) to test the potential for interspecific hybridization in Celtis breeding. AFLP profiles were used to assess the ancestry of progeny. Hybrids formed very rarely among these seven species of Celtis: only two interspecific hybrids were obtained. Self-pollination occurred occasionally in non-emasculated trees. AFLP analysis yielded false paternal markers at a very low frequency, likely due to DNA methylation differences. Plants with unexpected paternal markers were confidently distinguished from hybrids by calculating the probability of obtaining the observed number of paternal markers by chance. The study clearly demonstrated the importance of using large numbers of markers.

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Reproductive Efficiency in Reciprocal Crosses of Arachis monticola with A. hypogaea Subspecies1

Peanut Science ◽

10.3146/i0095-3679-25-1-3 ◽

1998 ◽

Vol 25 (1) ◽

pp. 7-12 ◽

Cited By ~ 6

Author(s):

H. E. Pattee ◽

H. T. Stalker ◽

F. G. Giesbrecht

Keyword(s):

New Mexico ◽

Ploidy Level ◽

Interspecific Hybrids ◽

Female Parent ◽

Reproductive Development ◽

Interspecific Crosses ◽

Reproductive Efficiency ◽

Embryo Abortion ◽

Reciprocal Crosses ◽

Cultivated Peanut

Abstract Wild species of Arachis encompass a large number of species which can provide valuable genetic resources for improving A. hypogaea L., the domesticated peanut. Arachis monticola Krapov. and Rig. is the only species which is both cross compatible with A. hypogaea and at the same ploidy level. An evaluation of reproductive efficiency in crosses between A. hypogaea and A. monticola was conducted to better understand the potential for utilization of this germplasm. This study documents the reproductive efficiency of A. monticola in reciprocal crosses with A. hypogaea subsp. hypogaea var. hypogaea cvs. Florunner and NC 6; A. hypogaea subsp. fastigiata var. vulgaris cv. Argentine; and A. hypogaea subsp. fastigiata var. fastigiata cv. New Mexico Valencia C by using selfs as controls. A significant maternal effect was observed among selfs and hybrids for timing of fertilization. Selfs of Florunner and New Mexico Valencia C initiated fertilization by 1 d after pollination, whereas syngamy did not occur in selfs of NC 6, Argentine or A. monticola until after day 1. Fertilization approached 100% in A. monticola and A. hypogaea genotypes except for New Mexico Valencia C, which only had 70% of the eggs fertilized. Embryo abortion was observed in both selfs and interspecific hybrids, with the highest rates in selfs after the pegs entered the soil; but in hybrids abortion also occurred as the peg elongated. Crosses were generally more successful when A. hypogaea was the female parent, and developing cultivars with A. monticola cytoplasm will be difficult. Sixty to more than 90% of growing ovules aborted in different interspecific crosses. Arachis monticola selfs and hybrids most closely followed the pattern of reproductive development of A. hypogaea cv. Argentine, which lends support to the theory that A. monticola is a weedy derivative of the cultivated peanut.

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