Immunoelectrophoretic analysis of major component proteins in cystic fluid of Taenia solium metacestodes

SummaryHuman endothelial cells isolated from umbilical cordswere solubilized in Triton X-100 and examined by crossedimmunoelec-trophoresis using rabbit antiserum against endothelial cells. Endogenous labelling of the endothelialcell proteins with 14Cmannose followed by crossed immunoelectrophoresis and autoradiography revealed about 10 immunoprecipitates. Four of these endothelial cell glycoproteins were labelled by lactoperoxidase catalyzed iodination and thus were surface located. Three of the surface located glycoproteins showed reduced electrophoretic mobility after incubation of the endothelial cells with neuraminidase and were therefore sialoglycoproteins. Amphiphilicity of endothelial cell glycoproteins was studied by crossed hydrophobic interaction immunoelectrophoresis with phenyl-Sepharose in the intermediate gel. Amphiphilic proteins also show increasing electrophoretic migration velocity with decreasing concentration of Triton X-100 in the first dimension gels. Five of the endothelial cell glycoproteins were shown to be amphiphilic using these two techniques.Two monoclonal antibodies against the platelet glycoprotein complex Ilb-IIIa and glycoprotein IlIa, respectively, reacted with the same precipitate of endothelial cells. When a polyclonal antibody against the platelet glycoprotein complex Ilb-IIIa was incorporated into the intermediate gel the position of two endothelial cell precipitates were lowered. One of these was a sialoglycoprotein.

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Acquired Disorder of Platelet Function Associated with Autoantibodies against Membrane Glycoprotein IIb-IIIa Complex - 1. Glycoprotein Analysis

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648178 ◽

1991 ◽

Vol 65 (05) ◽

pp. 491-496 ◽

Cited By ~ 29

Author(s):

M Meyer ◽

C M Kirchmaier ◽

A Schirmer ◽

P Spangenberg ◽

Ch Ströhl ◽

...

Keyword(s):

Platelet Count ◽

Platelet Aggregation ◽

Platelet Adhesion ◽

Bleeding Time ◽

Membrane Glycoprotein ◽

Membrane Glycoproteins ◽

Abnormal Behaviour ◽

Thrombocytopenic Purpura ◽

Immunoelectrophoretic Analysis ◽

Prolonged Bleeding Time

SummaryA patient with idiopathic thrombocytopenic purpura developed after splenectomy a thrombasthenia-like severe haemor-rhagic diathesis characterized by a normal or subnormal platelet count, prolonged bleeding time, strongly reduced platelet adhesion to glass and defective platelet aggregation in response to ADP and collagen. In contrast to hereditary thrombasthenia membrane glycoproteins (GP) lib and Ilia were normally present in the patient’s platelets. Immunoelectrophoretic analysis revealed an abnormal behaviour of the patient’s GP IIb-IIIa complex. Autoantibodies against GP IIb-IIIa were detected in Triton-extracted washed platelets. Incubation of normal platelets with plasma from the patient resulted in a similar immunoelectrophoretic abnormality of the GP IIb-IIIa complex indicating that bound autoantibodies (IgG) are responsible for the abnormal immunoelectrophoretic behaviour of the patient’s GP IIb-IIIa complex. Platelet fibrinogen was severely reduced similar to classical thrombasthenia suggesting that the GP IIb-IIIa complex is involved in platelet fibrinogen storage.

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INTESTINAL PARASITE INFECTION IN HUE UNIVERSITY HOSPITAL IN 2013-2014 BY DIRECT EXAMINATION AND IMMUNOLOGICAL DIAGNOSTICS

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2016.3.16 ◽

2016 ◽

pp. 105-111

Author(s):

Nu Phuong Anh Ton ◽

Thi Minh Chau Ngo

Keyword(s):

Intestinal Parasite ◽

Intestinal Parasites ◽

Clonorchis Sinensis ◽

Taenia Solium ◽

University Hospital ◽

High Rate ◽

Intestinal Helminth ◽

Direct Examination ◽

Life Condition ◽

Parasite Infections

Introduction: Intestinalparasite infections still are very common in tropical country such as Vietnam. Therefore evaluation of the prevalence of them should be done. Materials and methods: A crosssectional descriptivestudy were carried out to use the wet mount direct examination, Kato and ELISA technique for evaluation the rate of intestinal parasite infections, interviewed them to reveal their life’s hygenic condition, knowledge and risk behaviour of intestinal parasite infections in 640 patients attending to the in Hue University of hospital. Result: The rate of soiltransmitted intestinal helminth were lower than the foodborne infection of cestode and trematode such as: Taenia solium/saginata 3.64%, Clonorchis sinensis 2.73%. The rate of positive antibody of rare intestinal parasite were Fasciola gigantica 40.68%, E. histolytica/dispar 43.75%, Toxocara canis 32.43%, Gnasthostoma spinigerum 37.5%, Taenia solium 51.43%, Strongyloidesstercoralis 16.67%. Most of people has hygenic life condition with hygenic water available. The popular knowledge of intestinal parasite diseases of transmission, pathology, prevention and treatment were concentrate on oiltransmittedintestinal helminth.There were high rate of platyhelminths infectious behaviourssuch as: eating raw water plant 75.6%, eating raw beef 80.6%, eating raw pork 72.2%, eating raw fish 11.8%, bare foot 34.2%. Conclusions: The result of our study showed that the higher rate of foodborne cestode and trematode than soiltransmittedhelminth correspond tothe hygenic life condition, the knowledge of the diseases and risk behavior of intestinal parasites infection. Key words: Helminth, cestode, trematode

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