Comparative expression profiles of keratins and apoptosis regulating proteins in keratocystic odontogenic tumor, orthokeratinized odontogenic cyst, and dentigerous cyst

Abstract Objective Orthokeratinized odontogenic cyst is a rare developmental odontogenic cyst of the jaws. It is a less aggressive intraosseous cyst identified by an orthokeratinized epithelium. Case Report A 50-year-old male patient with the chief complaint of swelling in the anterior part of his face, and, intraorally, there was diffuse swelling in the palatal cortex. On panoramic radiography, there was a well-defined unilocular radiolucency on the right side of the maxilla and palatal cortical expansion, and thinning of the buccal and palatal cortexes was observed. The histopathological examination revealed a pathologic cyst that was lined by a thick orthokeratinized epithelium. Therefore, the diagnosis was orthokeratinized odontogenic cyst. Conclusion The orthokeratinized odontogenic cyst displays characteristic clinical, histopathological, and biological features that differ significantly from those of keratocystic odontogenic tumor (KCOT), but it has a better prognosis and lower recurrence rate. Thus, other radiolucent lesions of the jaws, including keratocystic odontogenic tumor (KCOT), must be considered in the differential diagnosis.

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Evaluation of the Connective Tissue Wall in Sporadic Cases of Keratocystic Odontogenic Tumor (KCOT) Using MMP-9 and Confocal Microscopy: A Retrospective Study

Journal of Advanced Oral Research ◽

10.1177/2320206820977676 ◽

2021 ◽

Vol 12 (1) ◽

pp. 134-143

Author(s):

Merlin Jayaraj ◽

Pratibha Ramani ◽

Herald J. Sherlin

Keyword(s):

Confocal Microscopy ◽

Connective Tissue ◽

Dentigerous Cyst ◽

Odontogenic Tumor ◽

Biological Behavior ◽

Keratocystic Odontogenic Tumor ◽

Extracellular Matrix Components ◽

Sporadic Cases ◽

Odontogenic Lesion ◽

Inherent Nature

Background: Keratocystic odontogenic tumor (KCOT) is an odontogenic lesion which manifests distinct biological behavior. Predominant studies in KCOT attribute this behavior to high epithelial proliferative capacity. Besides, a few studies facet loosely arranged collagen can contribute to the behavior of KCOT. Matrix metalloproteinases (MMP) are enzymes that degrade extracellular matrix components under both physiologic and pathologic conditions. The loosely arranged collagen in connective tissue wall of KCOT could be related to the degree of MMP-9 expression. Aim: To evaluate the arrangement of collagen fibers along with immunoexpression of MMP-9 and to relate to its neoplastic biologic behavior in sporadic cases of KCOT. Materials and Methods: KCOT ( n = 23) and dentigerous cyst (DC) ( n = 15) samples were processed for the following techniques: Masson’s trichrome stain for light microscopy, PMA-PSR stain for confocal microscopy, and MMP-9 for immunohistochemistry. Results: In Masson’s trichrome analysis, correlation of collagen fiber arrangement in the deeper regions with color intensity for KCOT was found to be statistically significant ( P = .033). In confocal microscopy, there was no difference between intensities in KCOT ( P = .990) and DC ( P = .233), respectively. The immunoexpression of MMP-9 in the connective tissue wall of DC (73.3%) was relatively higher than that of KCOT (60.8%). However, on comparison between KCOT and DC in the presence of inflammation, the immunoexpression of MMP-9 was higher in DC (100%) than KCOT (69.9%) and was statistically significant ( P = .028). Conclusion: It was concluded that the loose connective tissue wall in KCOT is because of the inherent nature of the lesion that could facilitate its biologic behavior. If inflammation is present, this could further aggravate the tumorigenic behavior.

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Orthokeratinized Odontogenic Cyst: A Clinicopathologic Study of 61 Cases

Archives of Pathology & Laboratory Medicine ◽

10.5858/134.2.271 ◽

2010 ◽

Vol 134 (2) ◽

pp. 271-275 ◽

Cited By ~ 10

Author(s):

Qing Dong ◽

Shuang Pan ◽

Li-Sha Sun ◽

Tie-Jun Li

Keyword(s):

Odontogenic Cyst ◽

World Health ◽

Keratocystic Odontogenic Tumor ◽

Impacted Tooth ◽

Ki 67 ◽

Orthokeratinized Odontogenic Cyst ◽

Clinicopathologic Study ◽

Mandibular Molar ◽

Health Organization

Abstract Context.—Orthokeratinized odontogenic cyst (OOC) is a relatively uncommon developmental cyst comprising about 10% of cases that had been previously coded as odontogenic keratocysts. Odontogenic keratocyst was designated as keratocystic odontogenic tumor (KCOT) in the new World Health Organization classification and OOC should be distinguished from KCOT for differences in histologic features and biologic behavior. Objective.—To analyze the clinicopathologic features of 61 cases of OOC in a Chinese population. Design.—Clinicopathologic analysis was performed on 61 cases of OOC. Immunohistochemical expression of Ki-67 and p63 was evaluated in 15 OOCs and 15 typical KCOTs. Results.—The 61 patients with OOC ranged from 13 to 75 years (average, 38.93 years). The lesions developed mainly in the third and fourth decades (57.38%) with a distinct predilection for males (72.13%). Six (9.84%) lesions were found in the maxilla and 55 (90.16%) in the mandible. The most common sites were in the mandibular molar and ramus region. Of the 54 cases with radiographic record, 47 (87.04%) were unilocular and 7 (12.96%) were multilocular radiolucencies. Twenty-seven of the 54 cysts were associated with an impacted tooth. Follow-up of 42 patients revealed no recurrence during an average period of 76.8 months after surgery. Compared with KCOTs, expression level of Ki-67 and p63 was significantly lower in OOCs, suggesting a lower proliferative activity. Conclusion.—Orthokeratinized odontogenic cyst is clinicopathologically distinct from KCOT and should constitute its own clinical entity.

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Orthokeratinized odontogenic cyst masquerading as dentigerous cyst

International Journal of Applied and Basic Medical Research ◽

10.4103/2229-516x.192597 ◽

2016 ◽

Vol 6 (4) ◽

pp. 297 ◽

Cited By ~ 3

Author(s):

Anshi Jain ◽

DeviCharan Shetty ◽

AjitSingh Rathore ◽

Natasha Thokchom ◽

Neha Khurana

Keyword(s):

Dentigerous Cyst ◽

Odontogenic Cyst ◽

Orthokeratinized Odontogenic Cyst

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The Possible Role of Mast Cells in the Odontogenic Cyst’s Pathogenesis: A Comparative Study between Dentigerous Cyst and Keratocystic Odontogenic Tumor

Pathology Research International ◽

10.1155/2016/8754567 ◽

2016 ◽

Vol 2016 ◽

pp. 1-4 ◽

Cited By ~ 2

Author(s):

Sareh Farhadi ◽

Fatemeh Shahsavari ◽

MirMahdi Davardan

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Dentigerous Cyst ◽

Test Method ◽

Odontogenic Tumor ◽

Blue Staining ◽

Keratocystic Odontogenic Tumor ◽

Wide Range ◽

Significant Difference ◽

Cell Densities

Background. Recently, mast cells were recognized in the pathogenesis of more aggressive pathologic lesions. This study was aimed to evaluate and compare the density of mast cells in Dentigerous cyst (DC) and Keratocystic odontogenic tumor (KCOT) regarding their different clinical behavior. Method. This study was conducted on 23 and 26 cases of DC and KCOT, respectively. Four-micron sections were prepared for Toluidine blue staining and mast cell densities in two desired cysts were studied. Final data was analyzed via t-test and Mann-Whitney U test method regarding the significant level lower than 0.05. Results. Mast cell densities were significantly higher in KCOTs for deep and superficial layers and both layers (P<0.05). The density of degranulated mast cells in the deeper layers and both layers was significantly higher in KCOTs (P<0.05). However, the density of degranulated mast cells in the superficial layer had no significant difference (P>0.05). Conclusion. It seems that mast cells may be involved in the pathogenesis of KCOT, but, regarding wide range of mast cell’s biologic activities, further investigations are recommended to confirm the issue and prepare the details.

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