scholarly journals High inductive magnetic stimuli and their effects on mesenchymal stromal cells, dendritic cells, and fibroblasts

2019 ◽  
pp. S433-S443
Author(s):  
J. Průcha ◽  
J. Skopalik ◽  
I. Justan ◽  
T. Parák ◽  
E. Gabrielová ◽  
...  

Effects of low-frequency electromagnetic fields (LF EMF) on the activation of different tissue recovery processes have already been fully understood. Preliminary recent data demonstrated that a special case of sinusoidal electromagnetic fields, known as amplitude-modulated currents (AMC) could have a potential to accelerate the cell metabolism or cell migration. An AMC generator was designed to generate sinusoidal induced electric currents with the amplitude modulation and the harmonic carrier frequency of 5,000 Hz was modulated by frequencies of 1 to 100 Hz. The magnetic field peak was 6 mT, electric field intensity 2 V/m and the current density of induced electrical currents was approximately 1 A/m2. The coil of the generator was adapted to easy handling and safe integration into the shelf of the CO2 incubator. The shelf with the coil was prepared for the introduction of cells in standard plastic in vitro chambers. The tests focused on cells with migratory capacity after injury or during immunological processes and thus, mesenchymal stromal cells (MSC), dendritic cells (DC), and fibroblasts were chosen. The tests involved exposures of the cells to LF EMF (180 min/day) every day, for a period of three days, before examining them for cell death, morphology changes, and CD markers. The samples were tested by using MTT assay and the effects on the intracellular concentration of reactive oxygen species were quantified. The cell migration was finally measured with the help of the transwell migration assay. None of the cell types showed any decrease in the cell viability after the LF EMF application and the cells displayed minimum changes in reactive oxygen species. Functional changes (acceleration of cell migration) after AMC exposure were statistically significant for the MSC samples only. The acceleration of MSCs is associated with the production of MMP by these cells. The EMF has a potential to be a safe, clinically applicable selective activator of MSC homing, MSC paracrine production, and subsequent regeneration processes.

2018 ◽  
Vol 1859 ◽  
pp. e45
Author(s):  
Sergiu Dumitrescu ◽  
Adelheid Weidinger ◽  
Asmita Banerjee ◽  
Susanne Wolbank ◽  
Karlheinz Hilber ◽  
...  

Human Cell ◽  
2021 ◽  
Author(s):  
Subodh Kumar ◽  
Ranjan Verma ◽  
Nishant Tyagi ◽  
Gurudutta Gangenahalli ◽  
Yogesh Kumar Verma

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Nabanita Kundu ◽  
Cleyton C. Domingues ◽  
Jay Patel ◽  
Mohammed Aljishi ◽  
Neeki Ahmadi ◽  
...  

2001 ◽  
Vol 16 (9) ◽  
pp. 1797-1801 ◽  
Author(s):  
Norihiro Sugino ◽  
Ayako Karube-Harada ◽  
Shiro Kashida ◽  
Shuji Takiguchi ◽  
Hiroshi Kato

2016 ◽  
Vol 84 (9) ◽  
pp. 2493-2504 ◽  
Author(s):  
Camaron R. Hole ◽  
Chrissy M. Leopold Wager ◽  
Andrew S. Mendiola ◽  
Karen L. Wozniak ◽  
Althea Campuzano ◽  
...  

Conventional dendritic cells (cDCs) are critical for protection against pulmonary infection with the opportunistic fungal pathogenCryptococcus neoformans; however, the role of plasmacytoid dendritic cells (pDCs) is unknown. We show for the first time that murine pDCs have direct activity againstC. neoformansvia reactive oxygen species (ROS), a mechanism different from that employed to controlAspergillus fumigatusinfections. The anticryptococcal activity of murine pDCs is independent of opsonization but appears to require the C-type lectin receptor Dectin-3, a receptor not previously evaluated during cryptococcal infections. Human pDCs can also inhibit cryptococcal growth by a mechanism similar to that of murine pDCs. Experimental pulmonary infection of mice with aC. neoformansstrain that induces protective immunity demonstrated that recruitment of pDCs to the lungs is CXCR3 dependent. Taken together, our results show that pDCs inhibitC. neoformansgrowthin vitrovia the production of ROS and that Dectin-3 is required for optimal growth-inhibitory activity.


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