scholarly journals Detection and Characterization of Botrytis cinerea Isolates from Vegetables in Egypt

2019 ◽  
Vol 8 (3) ◽  
pp. 77-85
Author(s):  
Mahmoud A Gaber ◽  
Elsayed E Wagih ◽  
Mohamed R. A Shehata ◽  
Magda M Fahmy ◽  
Hala Abdel Wahab
Keyword(s):  
Transposable Elements ◽  
Botrytis Cinerea ◽  
Plant Pathogen ◽  
Molecular Characteristics ◽  
Molecular Variation ◽  
Phenotypic Characteristic ◽  
Phenotypic Characteristics ◽  
Crop Losses

Botrytis cinerea is a necrotrophic plant pathogen that causes plenty of crop losses in Egypt and worldwide. Fifteen isolates of B. cinerea were collected from cabbage, pepper and lettuce grown in different locations in Egypt and subjected to investigation. Diversity in phenotypic, pathological and molecular characteristics was detected among isolates, leading to categorising them into four different groups. Molecular variation was demonstrated in all isolates by transposable elements (TEs) analyses. Four TE types, based on the presence or absence of two transposable elements, boty and flipper, were recognised among B. cinerea isolates in which transposa type (having both TE, boty + flipper) was predominant (40%), while only boty and only flipper types appeared with distribution values of 26.7 and 20%, respectively and vacuma type (Lacking both TEs) showed the lowest distribution value (13.3%). Furthermore, vacuma population demonstrated the lowest potential comparing to others. A correlation was found between TE type and virulence level of isolate, but no impact of TE type was observed on phenotypic characteristics of B. cinerea. The present study revealed a correlation between the TE type and the isolate virulence, but no correlation was observed between phenotypic characteristic/sensitivity to fenhexamid and TE type

Characterization of the cell wall of the ubiquitous plant pathogen Botrytis cinerea

2009 ◽  
Vol 113 (12) ◽  
pp. 1396-1403 ◽  
Author(s):  
Dario Cantu ◽  
L. Carl Greve ◽  
John M. Labavitch ◽  
Ann L.T. Powell
Keyword(s):  
Cell Wall ◽  
Botrytis Cinerea ◽  
Plant Pathogen

scholarly journals Phenotypic and genetic characterization of Botrytis cinerea isolates from tomato

Genetika ◽  
2012 ◽  
Vol 44 (3) ◽  
pp. 633-647 ◽  
Author(s):  
Biljana Kuzmanovska ◽  
Rade Rusevski ◽  
Ljupcho Jankuloski ◽  
Mirjana Jankulovska ◽  
Dario Ivic ◽  
...  
Keyword(s):  
Transposable Elements ◽  
Botrytis Cinerea ◽  
Genetic Characterization ◽  
Morphological Type ◽  
Republic Of Macedonia ◽  
High Tunnels ◽  
The Republic ◽  
Phenotypic And Genetic Characterization

One hundred and twenty-three isolates of Botrytis cinerea were collected from 7 different areas in the Republic of Macedonia, where tomato is mostly grown in greenhouses and high tunnels. Based on the mycelial formation, intensity of sporulation and sclerotial production, 9 different phenotypes were detected: 4 mycelial and 5 sclerotial. One sclerotial morphological type has not been previously reported. The presence or absence of two transposable elements, boty and flipper, was detected by PCR. Out of 123 isolates, 20 had two transposable elements, boty and flipper (transposa genotype), 48 had neither of these elements (vacuma genotype) and 55 had only the flipper element (flipper genotype). Isolates that contain only boty element were not detected. No relationship between the phenotypes, origin of isolates and the presence/absence of transposable elements, boty and flipper, was found.

scholarly journals Characterization of Botrytis cinerea isolates from small fruits and grapevine in Serbia

2009 ◽  
Vol 61 (3) ◽  
pp. 419-429 ◽  
Author(s):  
Brankica Tanovic ◽  
G. Delibasic ◽  
Jasminka Milivojevic ◽  
M. Nikolic
Keyword(s):  
Transposable Elements ◽  
Botrytis Cinerea ◽  
Phenotypic Variability ◽  
Morphological Characterization ◽  
Single Spore

Twenty-six single-spore isolates of Botrytis cinerea from blackberry, raspberry, strawberry, and grapevine were investigated using transposable elements, morphological characterization, and sensitivity to fungicides. Both transposable elements, Flipper and Boty, were detected among isolates from all the hosts. Six vacuma (without transposable elements) and seven transposa (containing both elements) isolates were found to be present in sympatry in Serbia. Isolates containing only the Boty element were detected. Eight morphological types of colonies on PDA and MA media were observed, confirming the great phenotypic variability of B. cinerea. Sensitivity to fungicides was various, depending on both the fungicide and the isolate.

scholarly journals Assessing the Effects of Light on Differentiation and Virulence of the Plant Pathogen Botrytis cinerea: Characterization of the White Collar Complex

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e84223 ◽  
Author(s):  
Paulo Canessa ◽  
Julia Schumacher ◽  
Montserrat A. Hevia ◽  
Paul Tudzynski ◽  
Luis F. Larrondo
Keyword(s):  
Botrytis Cinerea ◽  
Plant Pathogen ◽  
White Collar ◽  
White Collar Complex

scholarly journals Characterization of Postharvest Fungicide-Resistant Botrytis cinerea Isolates From Commercially Stored Apple Fruit

2017 ◽  
Vol 107 (3) ◽  
pp. 362-368 ◽  
Author(s):  
Wayne M. Jurick ◽  
Otilia Macarisin ◽  
Verneta L. Gaskins ◽  
Eunhee Park ◽  
Jiujiang Yu ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Gray Mold ◽  
Apple Fruit ◽  
Sublethal Dose ◽  
Long Term Storage ◽  
Pose Control ◽  
First Time

Botrytis cinerea causes gray mold and is an economically important postharvest pathogen of fruit, vegetables, and ornamentals. Fludioxonil-sensitive B. cinerea isolates were collected in 2011 and 2013 from commercial storage in Pennsylvania. Eight isolates had values for effective concentrations for inhibiting 50% of mycelial growth of 0.0004 to 0.0038 μg/ml for fludioxonil and were dual resistant to pyrimethanil and thiabendazole. Resistance was generated in vitro, following exposure to a sublethal dose of fludioxonil, in seven of eight dual-resistant B. cinerea isolates. Three vigorously growing B. cinerea isolates with multiresistance to postharvest fungicides were further characterized and found to be osmosensitive and retained resistance in the absence of selection pressure. A representative multiresistant B. cinerea strain caused decay on apple fruit treated with postharvest fungicides, which confirmed the in vitro results. The R632I mutation in the Mrr1 gene, associated with fludioxonil resistance in B. cinerea, was not detected in multipostharvest fungicide-resistant B. cinerea isolates, suggesting that the fungus may be using additional mechanisms to mediate resistance. Results from this study show for the first time that B. cinerea with dual resistance to pyrimethanil and thiabendazole can also rapidly develop resistance to fludioxonil, which may pose control challenges in the packinghouse environment and during long-term storage.

scholarly journals CHARACTERIZATION OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SCLAREOL AND 13-EPI-SCLAREOL, TWO LABDANE-TYPE DITERPENOIDS

2015 ◽  
Vol 60 (3) ◽  
pp. 3024-3028 ◽  
Author(s):  
LEONORA MENDOZA ◽  
CAROLINA SEPÚLVEDA ◽  
RICARDO MELO ◽  
MILENA COTORAS
Keyword(s):  
Antifungal Activity ◽  
Botrytis Cinerea

Characterization of Botrytis cinerea isolates collected on pepper in Southern Turkey by using molecular markers, fungicide resistance genes and virulence assay

2018 ◽  
Vol 60 ◽  
pp. 151-159 ◽  
Author(s):  
İlknur Polat ◽  
Ömür Baysal ◽  
Francesco Mercati ◽  
Emine Gümrükcü ◽  
Görkem Sülü ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Botrytis Cinerea ◽  
Resistance Genes ◽  
Fungicide Resistance ◽  
Virulence Assay ◽  
Southern Turkey

Characterization of a Vivid Homolog in Botrytis cinerea

2018 ◽  
Vol 94 (5) ◽  
pp. 985-993 ◽  
Author(s):  
Brandon J. Foley ◽  
Haley Stutts ◽  
Sydney L. Schmitt ◽  
Jameela Lokhandwala ◽  
Aditi Nagar ◽  
...  
Keyword(s):  
Botrytis Cinerea
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