Characterizing Illegal Harvest of the Venus' Fly Trap (Dionaea muscipula Ellis) at Lewis Ocean Bay Heritage Preserve, South Carolina, USA

2020 ◽  
Vol 40 (3) ◽  
Author(s):  
James O. Luken
2015 ◽  
Vol 18 (2) ◽  
pp. 99-104
Author(s):  
Uyen Hong Thu Vu ◽  
Trong Huu Nguyen ◽  
Phuc Hoang Tran ◽  
Le Van Bui

Dionaea muscipula, also known as Venus flytrap, is an endangered carnivorous plant which has origin from North and South Carolina, USA. An efficient protocol for largescale multiplication of this species has been set up through 3 stages: shoot multiplication, root induction and moving plant to the natural environment. On the half-strength Murashige and Skoog (MS) medium, supplemented with 0.5 mg/L kinetin gave the highest shoot proliferation of 20.44 ± 2.14 shoots/explant; adding 0.5 mg/l α - naphthaleneacetic acid (NAA) induced the best rooting 5.33 ± 0.44 roots/shoot. The 4-5 cm plantlets were then transplanted on the rooting medium including 50 % peat and 50 % perlite. In the first 14 days, they were placed in the light room with the application of an anti-transpirant film, irrigated 3 times/day and after that all moved to the garden. The rate of successful transfer process reached nearly 100 %.


Author(s):  
J. T. Ellzey ◽  
D. Borunda ◽  
B. P. Stewart

Genetically alcohol deficient deer mice (ADHN/ADHN) (obtained from the Peromyscus Genetic Stock Center, Univ. of South Carolina) lack hepatic cytosolic alcohol dehydrogenase. In order to determine if these deer mice would provide a model system for an ultrastructural study of the effects of ethanol on hepatocyte organelles, 75 micrographs of ADH+ adult male deer mice (n=5) were compared with 75 micrographs of ADH− adult male deer mice (n=5). A morphometric analysis of mitochondrial and peroxisomal parameters was undertaken.The livers were perfused with 0.1M HEPES buffer followed by 0.25% glutaraldehyde and 2% sucrose in 0.1M HEPES buffer (4C), removed, weighed and fixed by immersion in 2.5% glutaraldehyde in 0.1M HEPES buffer, pH 7.4, followed by a 3,3’ diaminobenzidine (DAB) incubation, postfixation with 2% OsO4, en bloc staining with 1% uranyl acetate in 0.025M maleate-NaOH buffer, dehydrated, embedded in Poly/Bed 812-BDMA epon resin, sectioned and poststained with uranyl acetate and lead citrate. Photographs were taken on a Zeiss EM-10 transmission electron microscope, scanned with a Howtek personal color scanner, analyzed with OPTIMAS 4.02 software on a Gateway2000 4DX2-66V personal computer and stored in Excel 4.0.


2009 ◽  
Vol 14 (2) ◽  
pp. 13-16
Author(s):  
Christopher R. Brigham ◽  
Jenny Walker

Abstract The AMAGuides to the Evaluation of Permanent Impairment (AMA Guides) is the most widely used basis for determining impairment and is used in state workers’ compensation systems, federal systems, automobile casualty, and personal injury, as well as by the majority of state workers’ compensation jurisdictions. Two tables summarize the edition of the AMA Guides used and provide information by state. The fifth edition (2000) is the most commonly used edition: California, Delaware, Georgia, Hawaii, Kentucky, New Hampshire, Idaho, Indiana, Iowa, Kentucky, Massachusetts, Nevada, North Dakota, Ohio, Vermont, and Washington. Eleven states use the sixth edition (2007): Alaska, Arizona, Louisiana, Mississippi, Montana, New Mexico, Oklahoma, Pennsylvania, Rhode Island, Tennessee, and Wyoming. Eight states still commonly make use of the fourth edition (1993): Alabama, Arkansas, Kansas, Maine, Maryland, South Dakota, Texas, and West Virginia. Two states use the Third Edition, Revised (1990): Colorado and Oregon. Connecticut does not stipulate which edition of the AMA Guides to use. Six states use their own state specific guidelines (Florida, Illinois, Minnesota, New York, North Carolina, and Wisconsin), and six states do not specify a specific guideline (Michigan, Missouri, Nebraska, New Jersey, South Carolina, and Virginia). Statutes may or may not specify which edition of the AMA Guides to use. Some states use their own guidelines for specific problems and use the Guides for other issues.


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