scholarly journals Micropropagation of venus flytrap Dionaea muscipula

2015 ◽  
Vol 18 (2) ◽  
pp. 99-104
Author(s):  
Uyen Hong Thu Vu ◽  
Trong Huu Nguyen ◽  
Phuc Hoang Tran ◽  
Le Van Bui
Keyword(s):  
South Carolina ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Carnivorous Plant ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Venus Flytrap ◽  
Dionaea Muscipula ◽  
Half Strength ◽  
Set Up

Dionaea muscipula, also known as Venus flytrap, is an endangered carnivorous plant which has origin from North and South Carolina, USA. An efficient protocol for largescale multiplication of this species has been set up through 3 stages: shoot multiplication, root induction and moving plant to the natural environment. On the half-strength Murashige and Skoog (MS) medium, supplemented with 0.5 mg/L kinetin gave the highest shoot proliferation of 20.44 ± 2.14 shoots/explant; adding 0.5 mg/l α - naphthaleneacetic acid (NAA) induced the best rooting 5.33 ± 0.44 roots/shoot. The 4-5 cm plantlets were then transplanted on the rooting medium including 50 % peat and 50 % perlite. In the first 14 days, they were placed in the light room with the application of an anti-transpirant film, irrigated 3 times/day and after that all moved to the garden. The rate of successful transfer process reached nearly 100 %.

scholarly journals In Vitro Clonal Propagation of Scoparia dulcis L., a Perennial Medicinal Herb

1970 ◽  
Vol 44 (3) ◽  
pp. 341-346
Author(s):  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
Nadira Begum ◽  
Rahima Khatun
Keyword(s):  
Clonal Propagation ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Naphthaleneacetic Acid ◽  
Scoparia Dulcis ◽  
Half Strength ◽  
In Vitro Clonal Propagation

An efficient protocol was established for in vitro clonal propagation of the perennial medicinal herb Scoparia dulcis L. (Family. Scrophulariaceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.1 mg/l BAP, in which 94% of the explants produced 12 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 16 shoots per culture. The half strength MS medium with 0.5 mg/l IBA +0.5 mg/l NAA the highest percentage (85.20) and maximum number (13.40) of roots were initiated within four weeks of culture. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Scoparia dulcis, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization, IAA (indoleacetic acid), IBA(indolebutanoic acid), NAA(α-naphthaleneacetic acid), BAP(benzylamino purine) DOI: 10.3329/bjsir.v44i3.4408 Bangladesh J. Sci. Ind. Res. 44(3), 341-346, 2009

scholarly journals In Vitro Propagation of HS314 Rootstock (Prunus amygdalus × P. persica)

HortScience ◽  
2011 ◽  
Vol 46 (6) ◽  
pp. 928-931
Author(s):  
Jalil Dejampour ◽  
Islam Majidi ◽  
Solmaz Khosravi ◽  
Sevil Farhadi ◽  
Atena Shadmehr
Keyword(s):  
Culture Media ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Prunus Amygdalus ◽  
Indole Butyric Acid ◽  
Half Strength

A micropropagation protocol was developed for the HS314 rootstock, a hybrid between almond and peach that could be used as an alternative rootstock instead of GF677. Surface-sterilized nodal segments were cultured in a modified DKW medium containing 3% sucrose, 100 mg·L−1 of Phloroglucinol, 0.7% plant agar, and 0.5 mg·L−1 benzyl amino purine (BAP). Explants were transferred to the same culture media supplemented with 0.5, 1, or 2 mg·L−1 BAP and 0, 0.1, or 0.5 mg·L−1 indole butyric acid (IBA) for further shoot proliferation. The maximum number of shoots produced on a medium containing 2 mg·L−1 BAP. Microshoots were transferred to the DKW medium supplemented with 0.5, 1, 2, or 4 mg·L−1 IBA or naphthaleneacetic acid (NAA) for root induction. The highest root number and the greatest root length were gained on a medium containing 2 mg·L−1 IBA. Rooting percentage was improved from 66% to more than 85% by reducing the concentration of DKW salts to half strength. Finally, rooted plantlets were successfully acclimatized and transferred in vivo conditions.

scholarly journals In Vitro Micropropagation of the Medicinal Plant Physalis angulata L.

2016 ◽  
Vol 8 (2) ◽  
pp. 161-163
Author(s):  
Owk ANIEL KUMAR ◽  
Songa RAMESH ◽  
Sape SUBBA TATA
Keyword(s):  
Large Scale ◽  
Leaf Disc ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Root Induction ◽  
Survival Percentage ◽  
Physalis Angulata

Physalis angulata L. is an important medicinal herb. An efficient direct adventitious plant regeneration protocol was developed for large scale propagation using leaf disc as explants. The explants were cultured on MS basal medium supplemented with 0.25-3.0 mg/L 6-benzyl amino purine (BAP) for primary shoot proliferation. Inclusion of indole-3-acetic acid (IAA) and gibberellic acid (GA3) in the culture medium along with BAP promoted a higher rate of shoot multiplication. The maximum number of shoots was produced in MS + BAP (1.0 mg/L) + IAA (0.5 mg/L) + GA3 (0.20 mg/L) after the third subculture. An average of 152.8 ± 0.40 shoots were produced from each leaf disc. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of indole-3-butyric acid (IBA). The highest percentage of root induction was observed in 1.0 mg/L (IBA). Rooted plants were successfully established in the soil after hardening. The survival percentage of rooted plants on soil was found to be 85%. This result will facilitate the conservation and propagation of the important medicinal herb Physalis angulata L.

scholarly journals Micropropagation of White Flowering Eastern Redbud (Cercis canadensis var. alba L.)

1990 ◽  
Vol 8 (4) ◽  
pp. 177-179
Author(s):  
S. Yusnita ◽  
R. L. Geneve ◽  
S. T. Kester
Keyword(s):  
Root System ◽  
Day Treatment ◽  
Shoot Multiplication ◽  
Naphthaleneacetic Acid ◽  
Indolebutyric Acid ◽  
Cercis Canadensis ◽  
Half Strength ◽  
Eastern Redbud ◽  
Being Moved

Abstract A white flowering Eastern redbud (Cercis canadensis var. alba L.) has been successfully micropropagated. Two node explants collected from the initial flush of spring growth were cultured on woody plant medium (WPM). Increased shoot multiplication occurred at 10,15 and 20 μM (2.3, 3.4 and 4.5 ppm) benzyladenine (BA). Microshoots were rooted in vitro on half strength WPM with a 15-day treatment of 100 and 300 μM (18.6 and 55.9 ppm) α-naphthaleneacetic acid (NAA) or 100 and 300 μM (20.3 and 60.9 ppm) indolebutyric acid (IBA) prior to being moved to full strength WPM without growth regulators. Percentage rooting and the mean number of roots per cutting were comparable between NAA and IBA treated microcuttings, however, the subsequent root morphology differed between the two treatments. NAA treated plants developed a coarse, unbranched root system, while IBA treated cuttings developed a more desireable fine, branched root system. Rooted microshoots were successfully acclimated to greenhouse conditions.

scholarly journals Micropropagation of Clerodendrum phlomidis L.F.

2016 ◽  
Vol 24 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Mafatlal M. Kher ◽  
Deepak Soner ◽  
Neha Srivastava ◽  
Murugan Nataraj ◽  
Jaime A. Teixeira da Silva
Keyword(s):  
Callus Formation ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Axillary Shoot Proliferation ◽  
Clerodendrum Phlomidis

Abstract Clerodendrum phlomidis L. f. is an important medicinal plant of the Lamiaceae family, particularly its roots, which are used for various therapeutic purposes in a pulverized form. The objective of this study was to develop a standard protocol for axillary shoot proliferation and rooting of C. phlomidis for its propagation and conservation. Nodal explants were inoculated on Murashige and Skoog (MS) medium that was supplemented with one of six cytokinins: 6-benzyladenine, kinetin, thidiazuron, N6-(2-isopentenyl) adenine (2iP), trans-zeatin (Zea) and meta-topolin. Callus induction, which was prolific at all concentrations, formed at the base of nodal explants and hindered shoot multiplication and elongation. To avoid or reduce callus formation with the objective of increasing shoot formation, the same six cytokinins were combined with 4 μM 2,3,5-tri-iodobenzoic acid (TIBA) alone or in combination with 270 μM adenine sulphate (AdS). Nodal explants that were cultured on the medium supplemented with 9.12 μM Zea, 4 μM TIBA and 270 μM AdS produced significantly more and longer shoots than on medium without TIBA and AdS. Half-strength MS medium supplemented with 8.05 μM α-naphthaleneacetic acid was the best medium for root formation. Most (75%) in vitro rooted plantlets were successfully acclimatized under natural conditions.

scholarly journals 333 Micropropagation of Echinacea angustifolia, E. pallida, and E. purpurea from Vegetative and Seed Explants

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 449C-449
Author(s):  
James F. Harbage
Keyword(s):  
Shoot Multiplication ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Root Induction ◽  
Echinacea Angustifolia ◽  
Adventitious Shoot Formation ◽  
Germplasm Preservation ◽  
In The Wild ◽  
Half Strength ◽  
Effective Use

Micropropagation of three Echinacea species, E. angustifolia, E. pallida, and E. purpurea, was investigated as a potential means of germplasm preservation of species faced with overcollection in the wild and rapid clonal propagation of elite individuals with unique medicinal or ornamental properties. Comparison of explant sources indicated vegetative explants resulted in high contamination rates when collected from shoot-tips (100%),but not when collected from nodal explants (11% to 39). Seed coat removal reduced contamination from 100% in intact seeds to near 0% in excised embryos. Removal of seed coats (pericarp and integument layers) also eliminated dormancy requirement for germination. All species responded with shoot multiplication and loss of rooting when BA or thidiazuron was added to culture medium. Medium with thidiazuron resulted in excessive adventitious shoot formation. Shoot multiplication rates were low (one to three shoots/explant) on medium with BA levels low enough to avoid adventitious shoot formation. Medium containing half-strength MS minerals resulted in more shoots with smaller leaves than full-strength MS minerals. Cultures did not perform well on Woody Plant Medium. Increasing subculture frequency from every 4 weeks to every 2 weeks increased shoot multiplication rates from 1.4 to 1.8 shoots per subculture and total shoots produced after 12 weeks of culturing (per initial explant) from 2.8 to 23.9. Rooting occurred readily on shoots isolated from E. purpurea without addition of IBA. Rooting was low or non-existent on shoots from E. angustifolia and E. pallida, respectively, regardless of IBA level, light conditions, or temperature. Methods described in this study allow rapid multiplication of three Echinacea species and subsequent rooting of E. angustifolia and E. purpurea. Future improvements in root induction treatments will allow more effective use of micropropagation for Echinacea germplasm preservation and multiplication. Chemical names used: N-(phenylmethyl)-1H-purine-6-amine (BA), 1H-indole-3-butyric acid (IBA).

scholarly journals Micropropagation of Two Selected Male Kiwifruit and Analysis of Genetic Variation with AFLP Markers

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 740-746 ◽  
Author(s):  
M.J. Prado ◽  
M.T. Herrera ◽  
R.A. Vázquez ◽  
S. Romo ◽  
M.V. González
Keyword(s):  
Genetic Variation ◽  
Gibberellic Acid ◽  
Shoot Multiplication ◽  
Aflp Markers ◽  
Naphthaleneacetic Acid ◽  
Multiplication Rate ◽  
Variation Analysis ◽  
Half Strength ◽  
Species Being ◽  
Regenerated Plantlets

A simple and reliable protocol for micropropagation during 12 subcultures of two field growth male plants of kiwifruit [Actinidia deliciosa (A.Chev.) Liang and Ferguson] is described. The best results of shoot multiplication and elongation were obtained in Cheng's K(h) medium in the presence of 0.5 μm NAA, 22 μm BA and 1.4 μm GA3 for `Tomuri' explants, and of 0.1 μm NAA, 4.4 μm BA, and 0.3 μm GA3 for clone A explants. In addition, the cytokinin compounds TDZ and mT were also tested allowing improving the multiplication rate in `Tomuri' explants. For rooting, `Tomuri' and clone A developed shoots were treated by basal immersion in a 5 mm IBA solution for 15 seconds. Treated shoots were then cultured in half-strength K(h) medium without growth regulators showing 100% rooting after 30 days. Regenerated plantlets were successfully transplanted to soil (90% survival) and they are actively growing in the field. Somaclonal variation analysis by AFLP was carried out using 15 primer combinations, yielding reproducible and well-resolved bands with a 57% of polymorphism. AFLP markers showed to be effective to discriminate genetic variation in this species, being greater in clone A than `Tomuri'. Chemical names used: N6-benzyladenine (BA); gibberellic acid (GA3); indole-3-butyric acid (IBA); meta-topolin (mT); naphthaleneacetic acid (NAA); thidiazuron (TDZ).

scholarly journals Micropropagation of Eclipta alba (Linn.) Hassk- a Valuable Medicinal Herb

1970 ◽  
Vol 43 (2) ◽  
pp. 215-222 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Farhana Afroz ◽  
Laila Shamroze Bari ◽  
John Liton Munshi ◽  
Miskat Ara Akhter Jahan ◽  
...  
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Mass Propagation ◽  
Eclipta Alba ◽  
Half Strength ◽  
Regenerated Plantlets

A protocol was established for mass propagation of a valuable medicinal herb, Eclipta alba (Linn.) Hassk (Asteraceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mgl-1 BAP + 0.1 mgl-1 NAA, in which 94% of the explants produced 18 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 26 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mgl-1 IBA +1.0 mgl-1 NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%. Key words: Eclipta alba, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization   DOI: 10.3329/bjsir.v43i2.965 Bangladesh J. Sci. Ind. Res. 43(2), 215-222, 2008 

scholarly journals Micropropagation and Assessment of Genetic Stability of Acclimatized Streptocarpus x hybridus Voss Plantlets Using RAPD Markers

2018 ◽  
Vol 75 (2) ◽  
Author(s):  
Monica HÂRŢA ◽  
Doina CLAPA ◽  
Orsolya BORSAI ◽  
Mihai Călin RUSU ◽  
Cristina KELEMEN ◽  
...  
Keyword(s):  
Rapd Markers ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
High Rate ◽  
Root Induction ◽  
Shoot Number ◽  
Indole 3 Acetic Acid ◽  
Very High

A micropropagation protocol via direct shoot organogenesis from Streptocarpus x hybridus Voss. leaf explants was established in this study. The shoot induction of three Streptocarpus cultivars (‘Snow White’, ‘Black Panther’ and ‘Slumber Song’) was successfully achieved on Murashige and Skoog (MS) medium supplemented with 0.2 mg L-1 -indole-3-acetic acid (IAA) and 0.2 mg L-1 thidiazuron (TDZ). In proliferation stage, the effects of two combinations of plant growth regulators -PGR- (V1-0.2 mg/L-1 IAA + 0.5 mg/L-1 BAP and V2-1.0 mg L-1 NAA + 0.2 mg L-1 TDZ) on shoot number and length were examined. The results suggest that PGRs combinations significantly influenced shoot proliferation and root induction in all Streptocarpus cultivars. Among the treatments, 0.2 mg L-1 (IAA) in combination with 0.5 mg L-1 6-benzylaminopurine (BAP) were the most effective for in vitro shoot multiplication and rooting. The in vitro rooting percentage was also determined before subjecting the plantlets to the acclimatization process. Due to acclimatization, Streptocarpus plantlets showed a very high rate of survival (90%). The generated PCR-RAPD profiles for the selected in vitro-raised plants and donor plants were similar which indicates the clonal or true-to-type nature of the progenies.

In vitro propagation of Epacris impressa (Epacridaceae) and the effects of clonal material

2000 ◽  
Vol 48 (2) ◽  
pp. 215 ◽  
Author(s):  
J. Anthony ◽  
C. B. McLean ◽  
A. C. Lawrie
Keyword(s):  
In Vitro Propagation ◽  
Root Formation ◽  
Woody Plant ◽  
Multiple Shooting ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Main Determinant ◽  
Woody Plant Medium ◽  
Half Strength

A system of micropropagation has been developed for Epacris impressa Labill. (pink heath) (Epacridaceae), the floral emblem of Victoria, Australia. Only explants from glasshouse-grown plants treated with 1.2 g L–1 mancozeb were established successfully in vitro. Shoot material was very sensitive to surface-sterilisation, with 0.5% NaOCl for 5 min being optimal. Multiple shooting was induced optimally on Woody Plant Medium (WPM, Lloyd and McCown 1980) with 12–25 µM of the cytokinin 2iP (6-(γ,γ-dimethylallylamino) purine). Inclusion of the auxin IBA (indole-3-butyric acid) induced callus and reduced shooting. Rooting in vitro was greatest (up to 40%) with half-strength WPM and 16 µM IBA. Clones from individual plants varied in multiple shooting response to 2iP (0–49 µM) and root induction response to auxins (IBA and NAA (α-naphthaleneacetic acid), 0–43 µM). These results suggest that explant materials are the main determinant of success in in vitro propagation and that they require individual optimisation of treatments to maximise shoot and root formation.

Sign in / Sign up

Export Citation Format

Share Document