scholarly journals A selenium-based cysteine surrogate for protein chemical synthesis

2021 ◽  
Author(s):  
Olga Firstova ◽  
Vangelis Agouridas ◽  
Vincent Diemer ◽  
Oleg Melnyk
Keyword(s):  
Chemical Synthesis ◽  
One Pot ◽  
Controlled Assembly ◽  
Peptide Segments

We provide in this protocol detailed procedures for the synthesis of SetCys cysteine surrogate and its use for chemical synthesis of proteins through the redox-controlled assembly of three peptide segments in one-pot.

One-Pot Glycosylation (OPG) for the Chemical Synthesis of Oligosaccharides

2005 ◽  
Vol 9 (2) ◽  
pp. 179-194 ◽  
Author(s):  
Biao Yu ◽  
Zunyi Yang ◽  
Hongzhi Cao
Keyword(s):  
Chemical Synthesis ◽  
One Pot

One-pot hydrazide-based native chemical ligation for efficient chemical synthesis and structure determination of toxin Mambalgin-1

2014 ◽  
Vol 50 (44) ◽  
pp. 5837-5839 ◽  
Author(s):  
Man Pan ◽  
Yao He ◽  
Ming Wen ◽  
Fangming Wu ◽  
Demeng Sun ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Snake Venom ◽  
Structure Determination ◽  
Native Chemical Ligation ◽  
One Pot ◽  
Chemical Ligation ◽  
Venom Toxin ◽  
Snake Venom Toxin ◽  
Switch Strategy

An efficient one-pot chemical synthesis of snake venom toxin Mambalgin-1 was achieved using an azide-switch strategy combined with hydrazide-based native chemical ligation.

scholarly journals A Straightforward Methodology to Overcome Solubility Challenges for N-Terminal Cysteinyl Peptide Segments Used in Native Chemical Ligation

2020 ◽  
Author(s):  
Skander Abboud ◽  
El hadji Cisse ◽  
Michel Doudeau ◽  
Hélène Bénédetti ◽  
Vincent AUCAGNE
Keyword(s):  
Amino Acids ◽  
Chemical Synthesis ◽  
Building Blocks ◽  
Native Chemical Ligation ◽  
Synthetic Approach ◽  
Chemical Ligation ◽  
Limited Solubility ◽  
Peptide Segments

One of the main limitations encountered during the chemical synthesis of proteins through native chemical ligation (NCL) is the limited solubility of some of the peptide segments. The most commonly used solution to overcome this problem is to derivatize the segment with a temporary solubilizing tag. Conveniently, the tag can be introduced on the thioester segment in such a way that it is removed concomitantly with the NCL reaction. We herein describe a generalization of this approach to N-terminal cysteinyl segment counterparts, using a straightforward synthetic approach that can be easily automated from commercially available building blocks, and applied it to a well-known problematic target, SUMO-2 (93 amino acids).

scholarly journals Multifunctional carbon nanotubes covalently coated with imine-based covalent organic frameworks: exploring structure–property relationships through nanomechanics

Nanoscale ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 1128-1137 ◽  
Author(s):  
Alicia Moya ◽  
Mercedes Hernando-Pérez ◽  
Marta Pérez-Illana ◽  
Carmen San Martín ◽  
Julio Gómez-Herrero ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Chemical Synthesis ◽  
Covalent Organic Frameworks ◽  
Structure Property ◽  
One Pot ◽  
Covalent Organic Framework ◽  
Mwcnt Surface ◽  
Structure Property Relationships ◽  
First Time ◽  
Organic Framework

Hybridization of imine-based covalent organic framework (COF-300) on oxidized MWCNT surface have been designed and succesfully developed for the first time in one-pot chemical synthesis.

Chemically synthesized circular RNAs with phosphoramidate linkages enable rolling circle translation

2020 ◽  
Vol 56 (46) ◽  
pp. 6217-6220
Author(s):  
Kosuke Nakamoto ◽  
Naoko Abe ◽  
Genichiro Tsuji ◽  
Yasuaki Kimura ◽  
Fumiaki Tomoike ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Stop Codon ◽  
Circular Rna ◽  
Circular Rnas ◽  
One Pot ◽  
Rolling Circle ◽  
Rna Fragments

Circular RNA without a stop codon enables rolling circle translation. we carried out one-pot chemical synthesis of circular RNA from RNA fragments. The synthesized circular RNAs acted as translation templates, despite the presence of unnatural phosphoramidate linkages.

scholarly journals Copper‐Mediated Selenazolidine Deprotection Enables One‐Pot Chemical Synthesis of Challenging Proteins

2019 ◽  
Vol 131 (41) ◽  
pp. 14752-14756 ◽  
Author(s):  
Zhenguang Zhao ◽  
Norman Metanis
Keyword(s):  
Chemical Synthesis ◽  
One Pot

One-Pot Glycosylation (OPG) for the Chemical Synthesis of Oligosaccharides

ChemInform ◽  
2005 ◽  
Vol 36 (46) ◽  
Author(s):  
Biao Yu ◽  
Zunyi Yang ◽  
Hongzhi Cao
Keyword(s):  
Chemical Synthesis ◽  
One Pot
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