scholarly journals Evaluation of whole-genome sequencing as a genotyping tool for Campylobacter jejuni in comparison with pulsed-field gel electrophoresis and flaA typing

2013 ◽  
Vol 92 (2) ◽  
pp. 573-580 ◽  
Author(s):  
S. Pendleton ◽  
I. Hanning ◽  
D. Biswas ◽  
S.C. Ricke
Keyword(s):  
Whole Genome Sequencing ◽  
Campylobacter Jejuni ◽  
Genome Sequencing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Whole Genome ◽  
Pulsed Field ◽  
Flaa Typing

scholarly journals Comparison of Pulsed-Field Gel Electrophoresis and Whole Genome Sequencing in Clostridium difficile Typing

2016 ◽  
Vol 3 (suppl_1) ◽  
Author(s):  
Ling Yuan Kong ◽  
David Eyre ◽  
A. Sarah Walker ◽  
Jacques Corbeil ◽  
Mark Wilcox ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Whole Genome ◽  
Pulsed Field

scholarly journals Whole genome sequencing provides an unambiguous link between Salmonella Dublin outbreak strain and a historical isolate

2015 ◽  
Vol 144 (3) ◽  
pp. 576-581 ◽  
Author(s):  
M. MOHAMMED ◽  
N. DELAPPE ◽  
J. O'CONNOR ◽  
P. McKEOWN ◽  
P. GARVEY ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Gel Electrophoresis ◽  
Invasive Disease ◽  
Pulsed Field Gel Electrophoresis ◽  
Whole Genome ◽  
Outbreak Strain ◽  
Salmonella Dublin ◽  
Source Of Infection ◽  
Human Salmonellosis

SUMMARYSalmonella enterica subsp. enterica serovar Dublin is an uncommon cause of human salmonellosis; however, a relatively high proportion of cases are associated with invasive disease. The serotype is associated with cattle. A geographically diffuse outbreak of S. Dublin involving nine patients occurred in Ireland in 2013. The source of infection was not identified. Typing of outbreak associated isolates by pulsed-field gel electrophoresis (PFGE) was of limited value because PFGE has limited discriminatory power for S. Dublin. Whole genome sequencing (WGS) showed conclusively that the isolates were closely related to each other, to an apparently unrelated isolate from 2011 and distinct from other isolates that were not readily distinguishable by PFGE.

scholarly journals Whole-Genome Sequencing to Detect Numerous Campylobacter jejuni Outbreaks and Match Patient Isolates to Sources, Denmark, 2015–2017

2020 ◽  
Vol 26 (3) ◽  
pp. 523-532 ◽  
Author(s):  
Katrine G. Joensen ◽  
Kristoffer Kiil ◽  
Mette R. Gantzhorn ◽  
Birgitte Nauerby ◽  
Jørgen Engberg ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Campylobacter Jejuni ◽  
Genome Sequencing ◽  
Whole Genome

scholarly journals Differentiation of Bacillus anthracis, B. cereus, and B. thuringiensis by Using Pulsed-Field Gel Electrophoresis

2007 ◽  
Vol 73 (10) ◽  
pp. 3446-3449 ◽  
Author(s):  
Wenwan Zhong ◽  
Yulin Shou ◽  
Thomas M. Yoshida ◽  
Babetta L. Marrone
Keyword(s):  
Cluster Analysis ◽  
Bacillus Anthracis ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Digestion ◽  
Whole Genome ◽  
Pulsed Field ◽  
High Profile

ABSTRACT A pulsed-field gel electrophoresis (PFGE) method was developed for discriminating Bacillus anthracis from B. cereus and B. thuringiensis. A worldwide collection of 25 B. anthracis isolates showed high-profile homology, and these isolates were unambiguously distinguished from B. cereus and B. thuringiensis isolates by cluster analysis of the whole-genome macrorestriction enzyme digestion patterns generated by NotI.

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