Discrimination by means of pulsed-field gel electrophoresis between strains of Campylobacter jejuni Lior type 4 derived from sporadic cases and from outbreaks of infection

1994 ◽  
Vol 29 (2) ◽  
pp. 183-187 ◽  
Author(s):  
Yasumoto Suzuki ◽  
Masamitsu Ishihara ◽  
Makoto Saito ◽  
Naohisa Ishikawa ◽  
Takashi Yokochi
Keyword(s):  
Campylobacter Jejuni ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Sporadic Cases

scholarly journals A Three-Year Study of Campylobacter jejuni Genotypes in Humans with Domestically Acquired Infections and in Chicken Samples from the Helsinki Area

2000 ◽  
Vol 38 (5) ◽  
pp. 1998-2000 ◽  
Author(s):  
Marja-Liisa Hänninen ◽  
Päivikki Perko-Mäkelä ◽  
Anna Pitkälä ◽  
Hilpi Rautelin
Keyword(s):  
Campylobacter Jejuni ◽  
Gel Electrophoresis ◽  
Peak Level ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Stool Samples

Campylobacter jejuni isolates from stool samples of patients with domestically acquired sporadic infections and from chicken from retail shops were studied during seasonal peaks from June to September over a 3-year period from 1996 to 1998. A large number of pulsed-field gel electrophoresis (PFGE) genotypes (a combinedSmaI-SacII pattern) were identified each year. Certain genotypes persisted for the whole study period, and predominant genotypes represented 28 to 52% of the strains during a restricted period of time. The peak level of positive chicken samples was between July and August of each study year, when 10 to 33% of the samples were positive for campylobacter. The same PFGE genotypes found in humans were also detected in the chicken samples. This suggests that common genotypes were circulating in the area.

scholarly journals Validity of SmaI-defined genotypes of Campylobacter jejuni examined by SalI, KpnI, and BamHI polymorphisms: evidence of identical clones infecting humans, poultry, and cattle

1998 ◽  
Vol 120 (3) ◽  
pp. 231-237 ◽  
Author(s):  
S. L. W. ON ◽  
E. M. NIELSEN ◽  
J. ENGBERG ◽  
M. MADSEN
Keyword(s):  
Campylobacter Jejuni ◽  
Gel Electrophoresis ◽  
Restriction Site ◽  
Epidemiological Studies ◽  
Pulsed Field Gel Electrophoresis ◽  
Genetic Differences ◽  
Genetic Fingerprinting ◽  
Molecular Subtyping ◽  
Pulsed Field ◽  
Heat Stable

We describe here an examination of the validity of molecular types of Campylobacter jejuni as defined by separation of SmaI-digested DNA using pulsed-field gel electrophoresis (PFGE), recently suggested as part of a molecular subtyping scheme. Thirty-four Danish strains from humans, water, poultry and cattle were assigned to one of six SmaI ‘profile groups’ (PGs), with two additional strains included as genotypically distinct controls. The interstrain relationships were reexamined by PFGE of SalI, KpnI and BamHI-digested DNA, and also by serotyping with heat-stable antigens. All outbreak-related strains were indistinguishable by all criteria, as were two sets of two randomly-isolated human strains. Two groups of indistinguishable isolates contained randomly isolated strains from more than one source (poultry, humans and/or cattle), a finding with significant epidemiological connotations. All ‘genetically identical’ strains belonged to the same serotype, whereas genetic differences were detected between strains assigned to the same SmaI PG but differing in serotype. We conclude that PFGE-based genetic fingerprinting can yield invaluable data for epidemiological studies of sporadic C. jejuni infection, but that results based on one restriction site polymorphism must be checked with another enzyme.

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