Impact of Lipid Peroxidation on the Physiology and Pathophysiology of Cell Membranes

Lipids ◽  
1990 ◽  
Vol 25 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Cesar G. Fraga ◽  
A. L. Tappel ◽  
Brian E. Leibovitz ◽  
Franz Kuypers ◽  
Daniel Chiu ◽  
...  

1998 ◽  
Vol 15 (Supplement 17) ◽  
pp. 16-17
Author(s):  
J. S. Althaus ◽  
E. D. Hall ◽  
P. F. VonVoigtlander

Author(s):  
T. Oguro ◽  
T. Onodera ◽  
M. Ashraf

Previously we reported that hydrogen peroxide (H2O2) is primarily cytotoxic to the cardiac cell membranes presumably through lipid peroxidation (LP). However, the effect of LP on the altered cell membrane permeability remains unknown. In this study, we evaluated the ultrastructural effects of LP on the sarcolemma of the isolated rat heart using H2O2 and N,N’-diphenyl-p-phenylene diamine (DPPD), an antilipid peroxidative agent.The hearts were perfused with 300 μM H2O2 for 15 minutes. In the other experiment the hearts were perfused with 2.5 μM DPPD for 20 minutes prior to perfusion with H2O2 for 15 minutes. Coronary effluent was collected at the end of the equilibration period and during H2O2 treatment for malondialdehyde (MDA) determination. Two minutes prior to the termination of the experiment, horseradish peroxidase (HRP: 220 U/mg × 55 mg) was perfused to assess the enhanced permeability of the cell membranes. The tissue slices were incubated in Graham-Karnovsky medium for one hour followed by postfixation with buffered 1% OsO4.


2021 ◽  
Vol 125 (38) ◽  
pp. 10736-10747
Author(s):  
Lingzhi Gu ◽  
Tong Wei ◽  
Mi Zhou ◽  
Hong Yang ◽  
Yang Zhou

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1185
Author(s):  
Nadia Gruber ◽  
Liliana Orelli ◽  
Cristina Minnelli ◽  
Luca Mangano ◽  
Emiliano Laudadio ◽  
...  

The potential of nitrones (N-oxides) as therapeutic antioxidants is due to their ability to counteract oxidative stress, mainly attributed to their action as radical scavengers toward C- and O-centered radicals. Among them, nitrones from the amidinoquinoxaline series resulted in interesting derivatives, due to the ease with which it is possible to introduce proper substituents within their structure in order to modulate their lipophilicity. The goal is to obtain lipophilic antioxidants that are able to interact with cell membranes and, at the same time, enough hydrophilic to neutralize those radicals present in a water compartment. In this work, the antioxidant efficacy of a series of amidinoquinoxaline nitrones has been evaluated regarding the oxidation of 2-deoxyribose and lipid peroxidation. The results have been rationalized on the basis of the different possible mechanisms involved, depending on some of their properties, such as lipophilicity, the ability to scavenge free radicals, and to undergo single electron transfer (SET) reactions.


2017 ◽  
Vol 44 (2) ◽  
pp. 618-633 ◽  
Author(s):  
Zhangyang Qi ◽  
Meihao Wu ◽  
Yun Fu ◽  
Tengfei Huang ◽  
Tingting Wang ◽  
...  

Background/Aims: The generation of reactive oxygen species (ROS) caused by amyloid-β (Aβ) is considered to be one of mechanisms underlying the development of Alzheimer’s disease. Curcumin can attenuate Aβ-induced neurotoxicity through ROS scavenging, but the protective effect of intracellular curcumin on neurocyte membranes against extracellular Aβ may be compromised. To address this issue, we synthesized a palmitic acid curcumin ester (P-curcumin) which can be cultivated on the cell membrane and investigated the neuroprotective effect of P-curcumin and its interaction with Aβ. Methods: P-curcumin was prepared through chemical synthesis. Its structure was determined via nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS). An MTT assay was used to assess Aβ cytotoxicity and the protective effect of P-curcumin on SH-SY5Y cells. The effect of P-curcumin on Aβ-induced ROS production in vitro and in vivo were assessed based on changes in dichlorofluorescein (DCF) fluorescence. A spectrophotometric method was employed to detect lipid peroxidation. To mimic the interaction of P-curcumin on cell membranes with Aβ, liposomes were prepared by thin film method. Finally, the interactions between free P-curcumin and P-curcumin cultivated on liposomes and Aβ were determined via spectrophotometry. Results: A novel derivative, palmitic acid curcumin ester was prepared and characterized. This curcumin, cultivated on the membranes of neurocytes, may prevent Aβ-mediated ROS production and may inhibit the direct interaction between Aβ and the cellular membrane. Furthermore, P-curcumin could scavenge Aβ-mediated ROS as curcumin in vitro and in vivo, and had the potential to prevent lipid peroxidation. Morphological analyses showed that P-curcumin was better than curcumin at protecting cell shape. To examine P-curcumin’s ability to attenuate direct interaction between Aβ and cell membranes, the binding affinity of Aβ to curcumin and P-curcumin was determined. The association constants for free P-curcumin and curcumin were 7.66 × 104 M-1 and 7.61 × 105 M-1, respectively. In the liposome-trapped state, the association constants were 3.71 × 105 M-1 for P-curcumin and 1.44× 106 M-1 for curcumin. With this data, the thermodynamic constants of P-curcumin association with soluble Aβ (ΔH, ΔS, and ΔG) were also determined. Conclusion: Cultivated curcumin weakened the direct interaction between Aβ and cell membranes and showed greater neuroprotective effects against Aβ insult than free curcumin.


1981 ◽  
Vol 92 (5) ◽  
pp. 1506-1508 ◽  
Author(s):  
M. L. Libe ◽  
E. D. Bogdanova ◽  
A. E. Rozenberg ◽  
L. L. Prilipko ◽  
V. E. Kagan ◽  
...  

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