scholarly journals A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging

2021 ◽  
Vol 8 ◽  
Author(s):  
Yubing Han ◽  
Zhimin Zhang ◽  
Wenjie Liu ◽  
Yuanfa Yao ◽  
Yingke Xu ◽  
...  

Despite the urgent need to image living specimens for cutting-edge biological research, most existing fluorescent labeling methods suffer from either poor optical properties or complicated operations required to realize cell-permeability and specificity. In this study, we introduce a method to overcome these limits—taking advantage of the intrinsic affinity of bright and photostable fluorophores, no matter if they are supposed to be live-cell incompatible or not. Incubated with living cells and tissues in particular conditions (concentration and temperature), some Atto and BODIPY dyes show live-cell labeling capability for specific organelles without physical cell-penetration or chemical modifications. Notably, by using Atto 647N as a live-cell mitochondrial marker, we obtain 2.5-time enhancement of brightness and photostability compared with the most commonly used SiR dye in long-term imaging. Our strategy has expanded the scientist's toolbox for understanding the dynamics and interactions of subcellular structures in living specimens.

2020 ◽  
Author(s):  
Yubing Han ◽  
Zhimin Zhang ◽  
Wenjie Liu ◽  
Yuanfa Yao ◽  
Yuchen Chen ◽  
...  

AbstractDespite the urgent needs of imaging living specimens for cutting-edge biological research, most of the existing fluorescent labeling methods suffer from either poor optical properties or complicated operations to realize cell-permeability and specificity. Here, we introduce a method to overcome this tradeoff by incubating living cells and tissues with fluorescent dyes, no matter if they are cell-permeable or not, at particular conditions (concentration and temperature) without physical cell-penetration or chemical modifications. Based on this method, the mitochondrial labeling capability of Atto dyes, especially Atto 647N with extraordinary optical properties, together with interesting interactions between organelles is revealed. These results indicate the great potential of using dyes, which are normally considered “live-cell incompatible”, to capture the morphology and dynamics of subcellular structures in living specimens.


2017 ◽  
Vol 23 (50) ◽  
pp. 12114-12119 ◽  
Author(s):  
Alexey N. Butkevich ◽  
Vladimir N. Belov ◽  
Kirill Kolmakov ◽  
Viktor V. Sokolov ◽  
Heydar Shojaei ◽  
...  

2014 ◽  
Vol 53 (8) ◽  
pp. 2245-2249 ◽  
Author(s):  
Ivana Nikić ◽  
Tilman Plass ◽  
Oliver Schraidt ◽  
Jędrzej Szymański ◽  
John A. G. Briggs ◽  
...  

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Gerti Beliu ◽  
Markus Sauer

AbstractNew peptide vehicles enable the efficient live-cell labeling of intracellular organelles with cell-impermeable fluorescent probes by simple coincubation, paving the way for refined multicolor super-resolution fluorescence imaging.


2015 ◽  
Vol 21 (35) ◽  
pp. n/a-n/a
Author(s):  
Jan-Erik Hoffmann ◽  
Tilman Plass ◽  
Ivana Nikić ◽  
Iker Valle Aramburu ◽  
Christine Koehler ◽  
...  

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0141918 ◽  
Author(s):  
Zhongqiu Ni ◽  
Lanxia Zhou ◽  
Xu Li ◽  
Jing Zhang ◽  
Shouliang Dong

2020 ◽  
Vol 2 (12) ◽  
pp. 5803-5809
Author(s):  
Lei Dong ◽  
Dehong Hu ◽  
Yanding Wang ◽  
Zonghai Sheng ◽  
Mei Hong ◽  
...  

The smallest-pore SAPO-20 zeolite confined pyrolysis of organics afforded ultrasmall uniform carbon dots with excellent performance in bioimaging.


2004 ◽  
Vol 16 (12) ◽  
pp. 961-966 ◽  
Author(s):  
A. M. Derfus ◽  
W. C. W. Chan ◽  
S. N. Bhatia

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