scholarly journals Design and Evaluation of a Novel Multiplex Real-Time PCR Melting Curve Assay for the Simultaneous Detection of Nine Sexually Transmitted Disease Pathogens in Genitourinary Secretions

2019 ◽  
Vol 9 ◽  
Author(s):  
Xiao-Mei Hu ◽  
Jiang-Xia Xu ◽  
Li-Xia Jiang ◽  
Lian-Rui Deng ◽  
Zhen-Mei Gu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sexually Transmitted Disease ◽  
Transmitted Disease ◽  
Simultaneous Detection ◽  
Melting Curve ◽  
Sexually Transmitted

scholarly journals Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Eliane Saori Otaguiri ◽  
Ana Elisa Belotto Morguette ◽  
Alexandre Tadachi Morey ◽  
Eliandro Reis Tavares ◽  
Gilselena Kerbauy ◽  
...  
Keyword(s):  
Real Time ◽  
Streptococcus Agalactiae ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Melting Curve ◽  
Pcr Assay ◽  
Genes Encoding

scholarly journals Development of Simple Multiplex Real-Time PCR Assays for Foodborne Pathogens Detection and Identification On Lightcycler

2017 ◽  
Vol 40 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Avo Karus ◽  
Fabrizio Ceciliani ◽  
Armand Sanches Bonastre ◽  
Virge Karus
Keyword(s):  
Real Time ◽  
Foodborne Pathogens ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Melting Curve ◽  
Food Contamination ◽  
Shigella Boydii ◽  
Food Borne Pathogens ◽  
Detection And Identification ◽  
Food Borne

Abstract Most acute intestinal diseases are caused by food-borne pathogens. A fast and simple real-time PCR-based procedure for simultaneous detection of food contamination by any of the five food-borne pathogens: Campylobacter jejuni, Mycobacterium bovis, Enterobacter sakazaki, Shigella boydii, Clostridium perfrigens using multiplex EvaGreen real-time PCR for LightCycler was developed and evaluated. Real-time qPCR showed excellent sensitivity. Tm calling and Melting Curve Genotyping (MCG) were used for analysis of PCR product melting curves. The Melting Curve Genotyping option showed good performance for discrimination of positive samples containing DNA of single pathogen or pathogen mixtures from negative samples.

Simultaneous Detection and Genotype Determination of HSV 1 and 2 by Real-time PCR Using Melting Curve Analysis and a Unique Pair of Primers

2017 ◽  
Vol 25 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Mahdi Paryan ◽  
Samira Mohammadi-Yeganeh ◽  
Houri Rezvan ◽  
Vahid Kia ◽  
Ardalan Mansouri ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Unique Pair ◽  
Hsv 1
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