scholarly journals Clinical Significance and Potential Role of LSM4 Overexpression in Hepatocellular Carcinoma: An Integrated Analysis Based on Multiple Databases

2022 ◽  
Vol 12 ◽  
Author(s):  
Liang Chen ◽  
Yun-hua Lin ◽  
Guo-qing Liu ◽  
Jing-en Huang ◽  
Wei Wei ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Cycle ◽  
Survival Analysis ◽  
Receiver Operating Characteristic ◽  
Clinical Significance ◽  
Focal Adhesion ◽  
Operating Characteristic ◽  
Curve Analysis ◽  
Diagnostic Significance ◽  
Receiver Operating

Background: Hepatocellular carcinoma (HCC) is a solid tumor with high recurrence rate and high mortality. It is crucial to discover available biomarkers to achieve early diagnosis and improve the prognosis. The effect of LSM4 in HCC still remains unrevealed. Our study is dedicated to exploring the expression of LSM4 in HCC, demonstrating its clinical significance and potential molecular mechanisms.Methods: Clinical information and LSM4 expression values of HCC were obtained from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. Survival analysis and receiver operating characteristic (ROC) curve analysis were applied to evaluate the prognostic and diagnostic significance of LSM4. Calculating pooled standardized mean difference (SMD) and performing summary receiver operating characteristic (sROC) curve analysis to further determine its expression status and diagnostic significance. LSM4-related co-expressed genes (CEGs) were obtained and explored their clinical significance in HCC. LSM4-associated pathways were identified through Gene set enrichment analysis (GSEA).Results: Up-regulated LSM4 was detected in HCC tissues (SMD = 1.56, 95% CI: 1.29–1.84) and overexpressed LSM4 had excellent distinguishing ability (AUC = 0.91, 95% CI: 0.88–0.93). LSM4 was associated with clinical stage, tumor grade, and lymph node metastasis status (p < 0.05). Survival analysis showed that high LSM4 expression was related to poor overall survival (OS) of HCC patients. Cox regression analysis suggested that high LSM4 expression may be an independent risk factor for HCC. We obtained nine up-regulated CEGs of LSM4 in HCC tissues, and six CEGs had good prognostic and diagnostic significance. GSEA analysis showed that up-regulated LSM4 was closely related to the cell cycle, cell replication, focal adhesion, and several metabolism-associated pathways, including fatty acid metabolism.Conclusion: Overexpressed LSM4 may serve as a promising diagnostic and prognostic biomarker of HCC. Besides, LSM4 may play a synergistic effect with CEGs in promoting the growth and metastasis of HCC cells via regulating crucial pathways such as cell cycle, focal adhesion, and metabolism-associated pathways.

A validated novel preoperative index to predict the extent of intraperitoneal contamination in patients with acute abdominal pathology: A cohort study

2019 ◽  
Vol 30 (7-8) ◽  
pp. 221-228
Author(s):  
Shahab Hajibandeh ◽  
Shahin Hajibandeh ◽  
Nicholas Hobbs ◽  
Jigar Shah ◽  
Matthew Harris ◽  
...  
Keyword(s):  
Receiver Operating Characteristic Curve ◽  
Receiver Operating Characteristic ◽  
Operating Characteristic ◽  
Validation Cohort ◽  
Characteristic Curve ◽  
Curve Analysis ◽  
Emergency Laparotomy ◽  
Contamination Index ◽  
Operating Characteristic Curve ◽  
Receiver Operating

Aims To investigate whether an intraperitoneal contamination index (ICI) derived from combined preoperative levels of C-reactive protein, lactate, neutrophils, lymphocytes and albumin could predict the extent of intraperitoneal contamination in patients with acute abdominal pathology. Methods Patients aged over 18 who underwent emergency laparotomy for acute abdominal pathology between January 2014 and October 2018 were randomly divided into primary and validation cohorts. The proposed intraperitoneal contamination index was calculated for each patient in each cohort. Receiver operating characteristic curve analysis was performed to determine discrimination of the index and cut-off values of preoperative intraperitoneal contamination index that could predict the extent of intraperitoneal contamination. Results Overall, 468 patients were included in this study; 234 in the primary cohort and 234 in the validation cohort. The analyses identified intraperitoneal contamination index of 24.77 and 24.32 as cut-off values for purulent contamination in the primary cohort (area under the curve (AUC): 0.73, P < 0.0001; sensitivity: 84%, specificity: 60%) and validation cohort (AUC: 0.83, P < 0.0001; sensitivity: 91%, specificity: 69%), respectively. Receiver operating characteristic curve analysis also identified intraperitoneal contamination index of 33.70 and 33.41 as cut-off values for feculent contamination in the primary cohort (AUC: 0.78, P < 0.0001; sensitivity: 87%, specificity: 64%) and validation cohort (AUC: 0.79, P < 0.0001; sensitivity: 86%, specificity: 73%), respectively. Conclusions As a predictive measure which is derived purely from biomarkers, intraperitoneal contamination index may be accurate enough to predict the extent of intraperitoneal contamination in patients with acute abdominal pathology and to facilitate decision-making together with clinical and radiological findings.

scholarly journals Adjustment for the measurement error in evaluating biomarker performances at baseline for future survival outcomes: Time-dependent receiver operating characteristic curve within a joint modelling framework

2020 ◽  
pp. 263208432097225
Author(s):  
Ruwanthi Kolamunnage-Dona ◽  
Adina Najwa Kamarudin
Keyword(s):  
Measurement Error ◽  
Receiver Operating Characteristic Curve ◽  
Receiver Operating Characteristic ◽  
Operating Characteristic ◽  
Characteristic Curve ◽  
Curve Analysis ◽  
Time Dependent ◽  
Survival Outcomes ◽  
Operating Characteristic Curve ◽  
Receiver Operating

The performance of a biomarker is defined by how well the biomarker is capable to distinguish between healthy and diseased individuals. This assessment is usually based on the baseline value of the biomarker; the value at the earliest time point of the patient follow-up, and quantified by ROC (receiver operating characteristic) curve analysis. However, the observed baseline value is often subjected to measurement error due to imperfect laboratory conditions and limited machine precision. Failing to adjust for measurement error may underestimate the true performance of the biomarker, and in a direct comparison, useful biomarkers could be overlooked. We develop a novel approach to account for measurement error when calculating the performance of the baseline biomarker value for future survival outcomes. We adopt a joint longitudinal and survival data modelling formulation and use the available longitudinally repeated values of the biomarker to make adjustment of the measurement error in time-dependent ROC curve analysis. Our simulation study shows that the proposed measurement error-adjusted estimator is more efficient for evaluating the performance of the biomarker than estimators ignoring the measurement error. The proposed method is illustrated using Mayo Clinic primary biliary cirrhosis (PBC) study.

P0742ASSOCIATION BETWEEN EOSINOPHILIA AND RENAL PROGNOSIS IN PATIENTS WITH CHOLESTEROL CRYSTAL EMBOLISM

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Yasuhiro Mochida ◽  
Takayasu Ohtake ◽  
Marie Morota ◽  
Kunihiro Ishioka ◽  
Hidekazu Moriya ◽  
...  
Keyword(s):  
Receiver Operating Characteristic ◽  
Operating Characteristic ◽  
Curve Analysis ◽  
Rank Test ◽  
Cholesterol Crystal ◽  
Renal Survival ◽  
Log Rank Test ◽  
Cholesterol Crystal Embolism ◽  
Renal Prognosis ◽  
Receiver Operating

Abstract Background and Aims Approximately, 20%-70% of patients with cholesterol crystal embolism (CCE) have eosinophilia. However, it remains unknown how eosinophilia influences on renal prognosis in patients with CCE. In this study, we investigated an association between eosinophil count (Eo) and renal prognosis in CCE patients on steroid therapy. Method The present study is a single-center retrospective cohort study in patients with pathological proven CCE and Chronic kidney disease from April 2007 to May 2018. This study included the patients who are not treated with maintenance dialysis nor steroid, and moreover followed until November 2019. We analyzed the validity of eosinophil counts using receiver operating characteristic (ROC) curve analysis. In the statistical analysis, renal survival was calculated with the Kaplan– Meier method, and comparisons between higher and low Eo groups were made with the log-rank test. Results Thirty-two patients with pathological diagnosed CCE were enrolled and followed-up for 11.0 (4.7-43.6) months. There were significant differences in the white blood cell (p=0.03), hemoglobin (p=0.007), serum creatinine levels (p=0.03), phosphate (p=0.045), Calcium×Phosphate (p=0.03), and Eo (p=0.016) between the renal survival and renal death groups. Using the receiver operating characteristic curve analysis with Youden index, Eo of 810/µL showed the sensitivity and specificity 71% and 88% for detecting renal death, respectively (area under the carve; 0.789). Comparing the outcomes in patients having Eo ≥ and &lt;810/µL by using the log-rank test, there are significantly higher renal death rate in CCE patients with Eo ≥810/µL (p=0.004). Conclusion Higher eosinophilia was a prognostic risk factor for renal death in the patients with CCE.

scholarly journals Evaluating Predictors of Apple Scab with Receiver Operating Characteristic Curve Analysis

2005 ◽  
Vol 95 (6) ◽  
pp. 679-691 ◽  
Author(s):  
William W. Turechek ◽  
Wayne F. Wilcox
Keyword(s):  
Receiver Operating Characteristic Curve ◽  
Receiver Operating Characteristic ◽  
Operating Characteristic ◽  
Characteristic Curve ◽  
Apple Scab ◽  
Curve Analysis ◽  
Immature Fruit ◽  
Action Threshold ◽  
Operating Characteristic Curve ◽  
Receiver Operating

Apple scab (Venturia inaequalis) is a perennial threat to apple production in temperate climates throughout the world. In the eastern United States, apple scab is managed almost exclusively through the regular application of fungicides. Management of the primary phase of disease is focused on preventing infection by ascospores. Management of secondary cycles of infection is largely dependent on how well primary infections were controlled. In this study, we used receiver operating characteristic curve analysis to evaluate how well mid-season assessments of the incidence of apple scab on cluster leaves, clusters (i.e., the whorl of cluster leaves), or immature fruit can serve as predictors of apple scab on harvested fruit (harvest scab) and whether these mid-season assessments of scab could be used reliably to manage scab under various damage thresholds. Results showed that assessment of scab on immature fruit was superior at predicting harvest scab than were assessments made on clusters or cluster leaves at all damage thresholds evaluated. A management action threshold of 7% scab incidence on immature fruit was identified by Youden's index as the optimal action threshold to prevent harvest scab incidence from exceeding 5%. Action thresholds could be higher or lower than 7% when economic assumptions were factored in to the decision process. The utility of such a predictor is discussed.

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