scholarly journals Transcriptomic Profiles of Senegalese Sole Infected With Nervous Necrosis Virus Reassortants Presenting Different Degree of Virulence

2018 ◽  
Vol 9 ◽  
Author(s):  
Alejandro M. Labella ◽  
Esther Garcia-Rosado ◽  
Isabel Bandín ◽  
Carlos P. Dopazo ◽  
Dolores Castro ◽  
...  
Keyword(s):  
Nervous Necrosis Virus ◽  
Senegalese Sole ◽  
Necrosis Virus

scholarly journals Immune Response of Senegalese Sole against Betanodavirus Mutants with Modified Virulence

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1388
Author(s):  
Juan Gémez-Mata ◽  
Sandra Souto ◽  
Isabel Bandín ◽  
María del Carmen Alonso ◽  
Juan José Borrego ◽  
...  
Keyword(s):  
Immune Response ◽  
Nervous Necrosis Virus ◽  
Senegalese Sole ◽  
Coding Region ◽  
Necrosis Virus ◽  
A Genome ◽  
Positive Sense ◽  
The Complement System ◽  
Reference Strains ◽  
Viral Encephalopathy And Retinopathy

Nervous necrosis virus (NNV), genus Betanodavirus, the etiological agent of the viral encephalopathy and retinopathy (VER), presents a genome with two positive-sense single-stranded RNA segments. Striped jack nervous necrosis virus (SJNNV) and red-spotted grouper nervous necrosis virus (RGNNV), together with reassortants RGNNV/SJNNV, are the betanodaviruses predominantly isolated in Southern Europe. An RGNNV/SJNNV reassortant isolated from Senegalese sole (wt160) causes high mortalities in this fish species. This virus presents differences in the sequence of the 3’ non-coding region (NCR) of both segments compared to RGNNV and SJNNV reference strains. Previously, it has been reported that the reversion of two of these differences (nucleotides 1408 and 1412) in the RNA2 3’NCR to the SJNNV-type (recombinant r1408-1412) resulted in a decrease in sole mortality. In the present study, we have applied an OpenArray® to analyse the involvement of sole immune response in the virulence of several recombinants: the r1408-1412 and two recombinants, developed in the present study, harbouring mutations at positions 3073 and 3093 of RNA1 3’NCR to revert them to RGNNV-type. According to the correlation values and to the number of expressed genes, the infection with the RNA2-mutant provoked the most different immune response compared to the immune response triggered after the infection with the rest of the viruses, and the exclusive and high upregulation of genes related to the complement system. The infection with the RNA1-mutants also provoked a decrease in mortality and their replication was delayed at least 24 h compared to the wt160 replication, which could provoke the lag observed in the immune response. Furthermore, the infection with the RNA1-mutants provoked the exclusive expression of pkr and the downregulation of il17rc.

Characterization of red-spotted grouper nervous necrosis virus isolated from ovarian fluids of asymptomatic wild Asian seabass, Lates calcarifer

Aquaculture ◽  
2021 ◽  
pp. 736846
Author(s):  
Venkata Satyanarayana Nallala ◽  
M. Makesh ◽  
K. Radhika ◽  
T. Sathish Kumar ◽  
P. Raja ◽  
...  
Keyword(s):  
Nervous Necrosis Virus ◽  
Necrosis Virus ◽  
Lates Calcarifer ◽  
Asian Seabass

Grouper MAVS functions as a crucial antiviral molecule against nervous necrosis virus infection

2018 ◽  
Vol 72 ◽  
pp. 14-22 ◽  
Author(s):  
Youhua Huang ◽  
Jingcheng Zhang ◽  
Zhengliang Ouyang ◽  
Jiaxin Liu ◽  
Ya Zhang ◽  
...  
Keyword(s):  
Virus Infection ◽  
Nervous Necrosis Virus ◽  
Necrosis Virus

In vitro neutralization of nervous necrosis virus by a nanobody binding to the protrusion domain of capsid protein

Aquaculture ◽  
2021 ◽  
pp. 737654
Author(s):  
Song Zhu ◽  
Bo Miao ◽  
Yu-Zhou Zhang ◽  
Wei-Wei Zeng ◽  
De-Shou Wang ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Nervous Necrosis Virus ◽  
Necrosis Virus

scholarly journals Dietary Administration of Novel Multistrain Probiotics from Healthy Grouper Intestines Promotes the Intestinal Immune Response against NNV Infection

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1053
Author(s):  
Joan Tang Xiao Joe ◽  
Henry Tan Shi Sung ◽  
Jen-Leih Wu ◽  
Yu-Shen Lai ◽  
Ming-Wei Lu
Keyword(s):  
Challenge Test ◽  
Antiviral Effect ◽  
Viral Disease ◽  
Growth Conditions ◽  
Asia Pacific ◽  
Nervous Necrosis Virus ◽  
Potential Candidate ◽  
Necrosis Virus ◽  
And Cluster Analysis ◽  
Functional Pathway

Epinephelus lanceolatus (giant grouper) is a high-value cultured species in the Asia-Pacific region. However, nervous necrosis virus (NNV) is an infectious viral disease that affects over 120 species of marine cultured species and causes high mortality, ranging from 90–100% in the grouper industry. Probiotics isolated from the intestines of healthy individuals have provided insight into novel approaches involved in the defense against viral pathogens. In this study, we isolated three strains of bacteria as candidate probiotics from healthy grouper intestines and a 28-day feeding trial was performed. At day 21, the nervous necrosis virus (NNV) challenge test was conducted for 7 days to evaluate the antiviral effect of candidate probiotics. The results showed that candidate probiotics could improve growth conditions, such as weight gain (WG) and specific growth rate (SGR), and increase the utilization of feed. Furthermore, the candidate probiotic mixture had the ability to protect against NNV, which could decrease the mortality rate by 100% in giant grouper after NNV challenge. Subsequently, we analyzed the mechanism of the candidate probiotic mixture’s defense against NNV. A volcano plot revealed 203 (control vs. NNV), 126 (NNV vs. probiotics–NNV), and 5 (control vs. probiotics–NNV) differentially expressed transcripts in intestinal tissue. Moreover, principal components analysis (PCA) and cluster analysis heatmap showed large differences among the three groups. Functional pathway analysis showed that the candidate probiotic mixture could induce the innate and adaptive immunity of the host to defend against virus pathogens. Therefore, we hope that potential candidate probiotics could be successfully applied to the industry to achieve sustainable aquaculture.

Sign in / Sign up

Export Citation Format

Share Document