scholarly journals A Flow Cytometry-Based Whole Blood Natural Killer Cell Cytotoxicity Assay Using Overnight Cytokine Activation

2020 ◽  
Vol 11 ◽  
Author(s):  
Jinho Kim ◽  
Minh-Trang Thi Phan ◽  
SoonHo Kweon ◽  
HongBi Yu ◽  
Jeehun Park ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Natural Killer Cell ◽  
Natural Killer ◽  
Whole Blood ◽  
Killer Cell ◽  
Cytotoxicity Assay ◽  
Cell Cytotoxicity ◽  
Natural Killer Cell Cytotoxicity ◽  
Cytokine Activation

Natural Killer Cell Cytotoxicity: A Methods Analysis of Versus Flow Cytometry Chromium Release

2003 ◽  
Vol 5 (2) ◽  
pp. 142-152 ◽  
Author(s):  
Sandra Adams Motzer ◽  
Joyce Tsuji ◽  
Vicky Hertig ◽  
Sandra K. Johnston ◽  
James Scanlan
Keyword(s):  
Flow Cytometry ◽  
Natural Killer ◽  
Mononuclear Cells ◽  
Nk Cell ◽  
Killer Cell ◽  
Cytotoxicity Assay ◽  
Cell Cytotoxicity ◽  
Peripheral Blood Mononuclear ◽  
Natural Killer Cell Cytotoxicity ◽  
Nk Cell Cytotoxicity

The purpose of this study is to describe design considerations for the use of flow cytometry (FC) com-pared to51chromium (51Cr)-release assays utilizing cryopreserved peripheral blood mononuclear cells (PBMCs) to detect natural killer (NK) cell cytotoxicity. Subjects were 10 healthy women aged 18 to 39 years. Intra-assay variability between methods differed only at the lowest effector-target ratios evaluated. Interassay variability was wide but did not differ between methods. The relationship of lytic unit-10 between methods was strongly positive. Cytotoxicity detected by51Cr release was higher than that detected by FC for all 10 subjects. Cost was comparable. How-ever, had more assays been performed, technician time would have been greater with flow cytometry. More whole blood was needed to perform the flow cytometry cytotoxicity assay than51Cr-release cytotoxicity assay. The authors found no compelling reason to adopt NK cell cytotoxicity by flow cytometry over51Cr release.

Ex vivo Natural Killer Cell Cytotoxicity Assay

BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (16) ◽  
Author(s):  
Lee-Hwa Tai ◽  
Christiano Souza ◽  
Andrew Makrigiannis ◽  
Rebecca Auer
Keyword(s):  
Natural Killer Cell ◽  
Natural Killer ◽  
Ex Vivo ◽  
Killer Cell ◽  
Cytotoxicity Assay ◽  
Cell Cytotoxicity ◽  
Natural Killer Cell Cytotoxicity

Measuring natural killer cell cytotoxicity by flow cytometry

Pathology ◽  
2019 ◽  
Vol 51 (3) ◽  
pp. 286-291 ◽  
Author(s):  
Wai-Yu Wong ◽  
Helen Wong ◽  
S.P. Cheung ◽  
Eric Chan
Keyword(s):  
Flow Cytometry ◽  
Natural Killer Cell ◽  
Natural Killer ◽  
Killer Cell ◽  
Cell Cytotoxicity ◽  
Natural Killer Cell Cytotoxicity

A regulatory loop among CD276, miR-29c-3p, and Myc exists in cancer cells against natural killer cell cytotoxicity

Life Sciences ◽  
2021 ◽  
Vol 277 ◽  
pp. 119438
Author(s):  
Chin-Cheng Lee ◽  
Kuo-Hao Ho ◽  
Tzu-Wen Huang ◽  
Chwen-Ming Shih ◽  
Shao-Yuan Hsu ◽  
...  
Keyword(s):  
Natural Killer Cell ◽  
Cancer Cells ◽  
Natural Killer ◽  
Killer Cell ◽  
Cell Cytotoxicity ◽  
Natural Killer Cell Cytotoxicity ◽  
Regulatory Loop

Effects of MR imaging on murine natural killer cell cytotoxicity

1987 ◽  
Vol 148 (2) ◽  
pp. 415-417 ◽  
Author(s):  
N Prasad ◽  
E Lotzova ◽  
JI Thornby ◽  
JE Madewell ◽  
JJ Ford ◽  
...  
Keyword(s):  
Natural Killer Cell ◽  
Mr Imaging ◽  
Natural Killer ◽  
Killer Cell ◽  
Cell Cytotoxicity ◽  
Natural Killer Cell Cytotoxicity

scholarly journals Dietary gluten increases natural killer cell cytotoxicity and cytokine secretion

2014 ◽  
Vol 44 (10) ◽  
pp. 3056-3067 ◽  
Author(s):  
Jesper Larsen ◽  
Morten Dall ◽  
Julie Christine Antvorskov ◽  
Christian Weile ◽  
Kåre Engkilde ◽  
...  
Keyword(s):  
Natural Killer Cell ◽  
Natural Killer ◽  
Killer Cell ◽  
Cytokine Secretion ◽  
Cell Cytotoxicity ◽  
Natural Killer Cell Cytotoxicity
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