Isolation and Characterization of the Novel Phages vB_VpS_BA3 and vB_VpS_CA8 for Lysing Vibrio parahaemolyticus

The increasing emergence of antimicrobial resistance has become a global issue. Therefore, many researchers have attempted to develop alternative antibiotics. One promising alternative is bacteriophage. In this study, we focused on a jumbo-phage infecting Salmonella isolated from exotic pet markets. Using a Salmonella strain isolated from reptiles as a host, we isolated and characterized the novel jumbo-bacteriophage pSal-SNUABM-04. This phage was investigated in terms of its morphology, host infectivity, growth and lysis kinetics, and genome. The phage was classified as Myoviridae based on its morphological traits and showed a comparatively wide host range. The lysis efficacy test showed that the phage can inhibit bacterial growth in the planktonic state. Genetic analysis revealed that the phage possesses a 239,626-base pair genome with 280 putative open reading frames, 76 of which have a predicted function and 195 of which have none. By genome comparison with other jumbo phages, the phage was designated as a novel member of Machinavirus composed of Erwnina phages.

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Isolation and Characterization of Bacteriophages from Inland Saline Aquaculture Environments to Control Vibrio parahaemolyticus Contamination in Shrimp

Indian Journal of Microbiology ◽

10.1007/s12088-021-00934-6 ◽

2021 ◽

Author(s):

Shiwam Dubey ◽

Ajeet Singh ◽

B. T. Naveen Kumar ◽

Niraj Kumar Singh ◽

Anuj Tyagi

Keyword(s):

Vibrio Parahaemolyticus ◽

Isolation And Characterization

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Isolation and Characterization of the Novel Lipophilic Protein, Pb CP-12.7, from the Shell of the Pink Shrimp, Pandalus borealis

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.65.1038 ◽

2001 ◽

Vol 65 (5) ◽

pp. 1038-1044 ◽

Cited By ~ 4

Author(s):

Kouichi SUZUKI ◽

Mariko OKAMORI ◽

Hirotaka KATSUZAKI ◽

Takashi KOMIYA ◽

Kunio IMAI

Keyword(s):

The Novel ◽

Pink Shrimp ◽

Pandalus Borealis ◽

Isolation And Characterization

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Isolation and characterization of authentic Phe-Met-Arg-Phe-NH2 and the novel Phe-Thr-Arg-Phe-NH2 peptide from Nereis diversicolor

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1991.tb16060.x ◽

1991 ◽

Vol 198 (3) ◽

pp. 627-633 ◽

Cited By ~ 20

Author(s):

Blandine BARATTE ◽

Helene GRAS-MASSE ◽

Guy RICART ◽

Philippe BULET ◽

Nicole DHAINAUT-COURTOIS

Keyword(s):

The Novel ◽

Nereis Diversicolor ◽

Isolation And Characterization

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Isolation and Characterization of a New Subtype of Borna Disease Virus

Journal of Virology ◽

10.1128/jvi.74.12.5655-5658.2000 ◽

2000 ◽

Vol 74 (12) ◽

pp. 5655-5658 ◽

Cited By ~ 68

Author(s):

Norbert Nowotny ◽

Jolanta Kolodziejek ◽

Christian O. Jehle ◽

Angelika Suchy ◽

Peter Staeheli ◽

...

Keyword(s):

Disease Virus ◽

Single Type ◽

The Novel ◽

Borna Disease Virus ◽

Diagnostic Assays ◽

Isolation And Characterization ◽

First Time ◽

Reference Strains ◽

Borna Disease

ABSTRACT Borna disease virus (BDV), the causative agent of severe meningoencephalitis in a wide variety of animal species, has been considered to be genetically invariable and to form a single type within the genus Bornavirus of the familyBornaviridae. BDV infections are of particular interest, because for the first time a virus infection appears to be linked to human psychiatric disorders. We now describe a new subtype of BDV isolated from a horse which was euthanatized due to severe, incurable neurological disease. The nucleotide sequence of this new strain, named No/98, differs from the reference strains by more than 15%, and the subtype is difficult to detect by standard reverse transcriptase PCR protocols. The nucleotide exchanges of the novel BDV isolate have surprisingly little effect on the primary structures of most viral proteins, with the notable exception of the X protein (p10), which is only 81% identical to its counterpart in reference strains. Our data indicate that the genome of BDV is far more variable than previously assumed and that naturally occurring subtypes may escape detection by currently used diagnostic assays.

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Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus

Frontiers in Microbiology ◽

10.3389/fmicb.2021.679245 ◽

2021 ◽

Vol 12 ◽

Author(s):

Galina Slobodkina ◽

Maxime Allioux ◽

Alexander Merkel ◽

Marie-Anne Cambon-Bonavita ◽

Karine Alain ◽

...

Keyword(s):

Deep Sea ◽

Hydrothermal Vent ◽

Single Copy ◽

Amino Acid Identity ◽

Hyperthermophilic Archaea ◽

The Novel ◽

Flagellar Motility ◽

Isolation And Characterization ◽

Mid Atlantic Ridge

Hyperthermophilic archaea of the genus Archaeoglobus are the subject of many fundamental and biotechnological researches. Despite their significance, the class Archaeoglobi is currently represented by only eight species obtained as axenic cultures and taxonomically characterized. Here, we report the isolation and characterization of a new species of Archaeoglobus from a deep-sea hydrothermal vent (Mid-Atlantic Ridge, TAG) for which the name Archaeoglobus neptunius sp. nov. is proposed. The type strain is SE56T (=DSM 110954T = VKM B-3474T). The cells of the novel isolate are motile irregular cocci growing at 50–85°C, pH 5.5–7.5, and NaCl concentrations of 1.5–4.5% (w/v). Strain SE56T grows lithoautotrophically with H2 as an electron donor, sulfite or thiosulfate as an electron acceptor, and CO2/HCO3− as a carbon source. It is also capable of chemoorganotrophic growth by reduction of sulfate, sulfite, or thiosulfate. The genome of the new isolate consists of a 2,115,826 bp chromosome with an overall G + C content of 46.0 mol%. The whole-genome annotation confirms the key metabolic features of the novel isolate demonstrated experimentally. Genome contains a complete set of genes involved in CO2 fixation via reductive acetyl-CoA pathway, gluconeogenesis, hydrogen and fatty acids oxidation, sulfate reduction, and flagellar motility. The phylogenomic reconstruction based on 122 conserved single-copy archaeal proteins supported by average nucleotide identity (ANI), average amino acid identity (AAI), and alignment fraction (AF) values, indicates a polyphyletic origin of the species currently included into the genus Archaeoglobus, warranting its reclassification.

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Isolation and Characterization of Vibrio parahaemolyticus in the hepatopancreas of cultured white pacific shrimp - Litopenaeus vannamei

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i1.1897 ◽

2020 ◽

Vol 11 (1) ◽

pp. 797-805

Author(s):

Kolli Guna Ranjan ◽

Girija Sankar G ◽

Satyanarayana Raju DVV

Keyword(s):

Vibrio Parahaemolyticus ◽

Bacterial Flora ◽

Distance Matrix ◽

Common Species ◽

Genetic Identification ◽

Molecular Technique ◽

Microbial Culture ◽

Biochemical Tests ◽

Isolation And Characterization

Vibrio parahaemolyticus is the most common species among crustaceans, often causing various diseases and significant losses in aquaculture. Acute hepatopancreatic necrosis disease (AHPND) is a newly emerging shrimp disease that has severely damaged the global shrimp industry. This species of bacteria is associated with gastrointestinal illness in humans and has been implicated in foodborne disease. The present study carried out, isolation and characterization of pathogenic bacterial flora isolated from the infected hepatopancreas of vannamei, obtained from various aquafarms in Andhra Pradesh, India, on 11th June 2018. The collected samples were plated on TCBS- (Thiosulfate-Citrate-Bile salt-Sucrose) agar medium and Hi -Chrome vibrio, as described in Bergey's manual of systematic bacteriology. Isolated colonies were subjected to the following tests- microscopic examination, growth at different temperatures, growth at different NaCl concentrations, and biochemical tests. Further purity, maintenance, and propagation of purified cultures were done. The microbial culture was identified using 16s rRNA molecular technique. Phylogenetic Evolutionary analyses and distance matrix were conducted in MEGA7.In the present study, different samples were screened, a total of three green colonies (V44, V45, V46) were isolated, identified by biochemical tests and genetic identification as Vibrio parahaemolyticus. A systematic methodology has been developed to isolate and characterize Vibrio sp. from diseased shrimp and identify them by genetic analysis

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