Isolation and Characterization of the Novel Lipophilic Protein, Pb CP-12.7, from the Shell of the Pink Shrimp, Pandalus borealis

The increasing emergence of antimicrobial resistance has become a global issue. Therefore, many researchers have attempted to develop alternative antibiotics. One promising alternative is bacteriophage. In this study, we focused on a jumbo-phage infecting Salmonella isolated from exotic pet markets. Using a Salmonella strain isolated from reptiles as a host, we isolated and characterized the novel jumbo-bacteriophage pSal-SNUABM-04. This phage was investigated in terms of its morphology, host infectivity, growth and lysis kinetics, and genome. The phage was classified as Myoviridae based on its morphological traits and showed a comparatively wide host range. The lysis efficacy test showed that the phage can inhibit bacterial growth in the planktonic state. Genetic analysis revealed that the phage possesses a 239,626-base pair genome with 280 putative open reading frames, 76 of which have a predicted function and 195 of which have none. By genome comparison with other jumbo phages, the phage was designated as a novel member of Machinavirus composed of Erwnina phages.

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Isolation and characterization of authentic Phe-Met-Arg-Phe-NH2 and the novel Phe-Thr-Arg-Phe-NH2 peptide from Nereis diversicolor

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1991.tb16060.x ◽

1991 ◽

Vol 198 (3) ◽

pp. 627-633 ◽

Cited By ~ 20

Author(s):

Blandine BARATTE ◽

Helene GRAS-MASSE ◽

Guy RICART ◽

Philippe BULET ◽

Nicole DHAINAUT-COURTOIS

Keyword(s):

The Novel ◽

Nereis Diversicolor ◽

Isolation And Characterization

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Isolation and Characterization of a New Subtype of Borna Disease Virus

Journal of Virology ◽

10.1128/jvi.74.12.5655-5658.2000 ◽

2000 ◽

Vol 74 (12) ◽

pp. 5655-5658 ◽

Cited By ~ 68

Author(s):

Norbert Nowotny ◽

Jolanta Kolodziejek ◽

Christian O. Jehle ◽

Angelika Suchy ◽

Peter Staeheli ◽

...

Keyword(s):

Disease Virus ◽

Single Type ◽

The Novel ◽

Borna Disease Virus ◽

Diagnostic Assays ◽

Isolation And Characterization ◽

First Time ◽

Reference Strains ◽

Borna Disease

ABSTRACT Borna disease virus (BDV), the causative agent of severe meningoencephalitis in a wide variety of animal species, has been considered to be genetically invariable and to form a single type within the genus Bornavirus of the familyBornaviridae. BDV infections are of particular interest, because for the first time a virus infection appears to be linked to human psychiatric disorders. We now describe a new subtype of BDV isolated from a horse which was euthanatized due to severe, incurable neurological disease. The nucleotide sequence of this new strain, named No/98, differs from the reference strains by more than 15%, and the subtype is difficult to detect by standard reverse transcriptase PCR protocols. The nucleotide exchanges of the novel BDV isolate have surprisingly little effect on the primary structures of most viral proteins, with the notable exception of the X protein (p10), which is only 81% identical to its counterpart in reference strains. Our data indicate that the genome of BDV is far more variable than previously assumed and that naturally occurring subtypes may escape detection by currently used diagnostic assays.

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Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus

Frontiers in Microbiology ◽

10.3389/fmicb.2021.679245 ◽

2021 ◽

Vol 12 ◽

Author(s):

Galina Slobodkina ◽

Maxime Allioux ◽

Alexander Merkel ◽

Marie-Anne Cambon-Bonavita ◽

Karine Alain ◽

...

Keyword(s):

Deep Sea ◽

Hydrothermal Vent ◽

Single Copy ◽

Amino Acid Identity ◽

Hyperthermophilic Archaea ◽

The Novel ◽

Flagellar Motility ◽

Isolation And Characterization ◽

Mid Atlantic Ridge

Hyperthermophilic archaea of the genus Archaeoglobus are the subject of many fundamental and biotechnological researches. Despite their significance, the class Archaeoglobi is currently represented by only eight species obtained as axenic cultures and taxonomically characterized. Here, we report the isolation and characterization of a new species of Archaeoglobus from a deep-sea hydrothermal vent (Mid-Atlantic Ridge, TAG) for which the name Archaeoglobus neptunius sp. nov. is proposed. The type strain is SE56T (=DSM 110954T = VKM B-3474T). The cells of the novel isolate are motile irregular cocci growing at 50–85°C, pH 5.5–7.5, and NaCl concentrations of 1.5–4.5% (w/v). Strain SE56T grows lithoautotrophically with H2 as an electron donor, sulfite or thiosulfate as an electron acceptor, and CO2/HCO3− as a carbon source. It is also capable of chemoorganotrophic growth by reduction of sulfate, sulfite, or thiosulfate. The genome of the new isolate consists of a 2,115,826 bp chromosome with an overall G + C content of 46.0 mol%. The whole-genome annotation confirms the key metabolic features of the novel isolate demonstrated experimentally. Genome contains a complete set of genes involved in CO2 fixation via reductive acetyl-CoA pathway, gluconeogenesis, hydrogen and fatty acids oxidation, sulfate reduction, and flagellar motility. The phylogenomic reconstruction based on 122 conserved single-copy archaeal proteins supported by average nucleotide identity (ANI), average amino acid identity (AAI), and alignment fraction (AF) values, indicates a polyphyletic origin of the species currently included into the genus Archaeoglobus, warranting its reclassification.

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Isolation and characterization of the novel Virgibacillus-infecting bacteriophage Mimir87

Archives of Virology ◽

10.1007/s00705-019-04516-2 ◽

2019 ◽

Vol 165 (3) ◽

pp. 737-741 ◽

Cited By ~ 2

Author(s):

Nikita Zrelovs ◽

Elina Cernooka ◽

Andris Dislers ◽

Andris Kazaks

Keyword(s):

The Novel ◽

Isolation And Characterization

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Novel Antiplasmodial Agents from Christia vespertilionis

Natural Product Communications ◽

10.1177/1934578x1300801123 ◽

2013 ◽

Vol 8 (11) ◽

pp. 1934578X1300801 ◽

Cited By ~ 3

Author(s):

Harish C. Upadhyay ◽

Brijesh S. Sisodia ◽

Harveer S. Cheema ◽

Jyoti Agrawal ◽

Anirban Pal ◽

...

Keyword(s):

Antimalarial Activity ◽

Complete Suppression ◽

The Novel ◽

Aqueous Methanol ◽

Isolation And Characterization ◽

First Time ◽

Detailed Chemical

The roots, leaves and stems of Christia vespertilionis were separately and successively extracted with methanol and aqueous-methanol (1:4, v/v) and were evaluated in vitro for their antiplasmodial potential against Plasmodium falciparum NF-54. The aqueous-methanolic stem (AS) extract was the most active (IC50 7.5 μg/mL) followed by the methanolic leaf (ML) extract (IC50 32.0 μg/mL). The in vivo antimalarial activity of the combined plant extract of C. vespertilionis was also assessed in P. berghei infected mice, which showed 87.8% suppression of parasitaemia as compared with complete suppression by chloroquine on day 8. Finally, detailed chemical investigation of C. vespertilionis resulted in the isolation and characterization of fifteen compounds (1–15), of which two (1 and 4) are being reported for the first time from nature. The novel compound 1 possesses potent antiplasmodial activity (IC50 = 9.0 μg/mL).

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Acidic C−H Activation of Methyltrioxorhenium(VII): Isolation and Characterization of Two Compounds Having the Novel Group C5H5N−CH2−ReO2Coordinated to Tin(IV)

Organometallics ◽

10.1021/om000683r ◽

2000 ◽

Vol 19 (25) ◽

pp. 5257-5259 ◽

Cited By ~ 10

Author(s):

Cungen Zhang ◽

Ilia A. Guzei ◽

James H. Espenson

Keyword(s):

The Novel ◽

Isolation And Characterization

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Isolation and Characterization of Isofraxidin 7-O-(6′-O-p-Coumaroyl)-β-glucopyranoside from Artemisia capillaris Thunberg: A Novel, Nontoxic Hyperpigmentation Agent That Is Effective In Vivo

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/1401279 ◽

2017 ◽

Vol 2017 ◽

pp. 1-12 ◽

Cited By ~ 7

Author(s):

Soon-Ho Yim ◽

Nadia Tabassum ◽

Woong-Hee Kim ◽

Haaglim Cho ◽

Ji-Hyung Lee ◽

...

Keyword(s):

Skin Pigmentation ◽

Parent Compound ◽

Tyrosinase Activity ◽

The Novel ◽

Developmental Defects ◽

Artemisia Capillaris ◽

Isolation And Characterization ◽

Vertebrate Model

Abnormalities in skin pigmentation can produce disorders such as albinism or melasma. There is a research need to discover novel compounds that safely and effectively regulate pigmentation. To identify novel modulators of pigmentation, we attempted to purify compounds from a bioactive fraction of the Korean medicinal plant Artemisia capillaris Thunberg. The novel compound isofraxidin 7-O-(6′-O-p-coumaroyl)-β-glucopyranoside (compound 1) was isolated and its pigmentation activity was characterized in mammalian melanocytes. Compound 1 stimulated melanin accumulation and increased tyrosinase activity, which regulates melanin synthesis. Moreover, compound 1 increased the expression of tyrosinase and the key melanogenesis regulator microphthalmia-associated transcription factor (MITF) in melanocytes. Compared to the parent compound, isofraxidin, compound 1 produced greater effects on these pigmentation parameters. To validate compound 1 as a novel hyperpigmentation agent in vivo, we utilized the zebrafish vertebrate model. Zebrafish treated with compound 1 showed higher melanogenesis and increased tyrosinase activity. Compound 1 treated embryos had no developmental defects and displayed normal cardiac function, indicating that this compound enhanced pigmentation without producing toxicity. In summary, our results describe the characterization of novel natural product compound 1 and its bioactivity as a pigmentation enhancer, demonstrating its potential as a therapeutic to treat hypopigmentation disorders.

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Isolation And Characterization Of 45 SNP Markers In Triplophysa Tenuis

10.21203/rs.3.rs-551660/v1 ◽

2021 ◽

Author(s):

Ya Liu ◽

Yeyu Chen ◽

Jiansheng Lai ◽

Hongyu Ke ◽

Zhongmeng Zhao ◽

...

Keyword(s):

Polymorphic Information Content ◽

Snp Markers ◽

Gansu Province ◽

The Novel ◽

Genetic Management ◽

Polymorphic Snps ◽

Isolation And Characterization ◽

Population Conservation ◽

Hardy Weinberg Equilibrium

Abstract Triplophysa tenuis is an endemic species to China, which mainly distributed in Xinjiang and Gansu province. Effective conservation and management of this species is limited by insufficient molecular markers. In the present study, we reported the isolation and characterization of 45 SNP markers in T. tenuis. The minor allele frequency ranged from 0.046 to 0.500, and the observed and expected heterozygosities ranged from 0.061 to 0.667 and 0.088 to 0.508, respectively. Polymorphic information content ranged from 0.083 to 0.375. Among these SNPs, three loci showed significant departures from the Hardy–Weinberg equilibrium. The novel polymorphic SNPs will be helpful for the future study on genetic management and population conservation for this species.

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Tiger team: a panel of human neutralizing mAbs targeting SARS-CoV-2 spike at multiple epitopes

10.1101/2020.05.20.106609 ◽

2020 ◽

Author(s):

Tal Noy-Porat ◽

Efi Makdasi ◽

Ron Alcalay ◽

Adva Mechaly ◽

Yinon Levy ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Neutralizing Antibodies ◽

Phage Display Library ◽

Therapeutic Drug ◽

The Novel ◽

Receptor Binding Domain ◽

Human Coronavirus ◽

Isolation And Characterization ◽

Emerging Virus

AbstractThe novel highly transmissible human coronavirus SARS-CoV-2 is the causative agent of the COVID-19 pandemic. Thus far, there is no approved therapeutic drug, specifically targeting this emerging virus. Here we report the isolation and characterization of a panel of human neutralizing monoclonal antibodies targeting the SARS-CoV-2 receptor binding domain (RBD). These antibodies were selected from a phage display library constructed using peripheral circulatory lymphocytes collected from patients at the acute phase of the disease. These neutralizing antibodies are shown to recognize distinct epitopes on the viral spike RBD, therefore they represent a promising basis for the design of efficient combined post-exposure therapy for SARS-CoV-2 infection.

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