scholarly journals Encapsulation of B. bassiana in Biopolymers: Improving Microbiology of Insect Pest Control

2021 ◽  
Vol 12 ◽  
Author(s):  
Ana Paula Felizatti ◽  
Roberta Maria Manzano ◽  
Inajá Marchizeli Wenzel Rodrigues ◽  
Maria Fátima das Graças Fernandes da Silva ◽  
João Batista Fernandes ◽  
...  
Keyword(s):  
Spray Drying ◽  
Pest Control ◽  
Gas Flow ◽  
Insect Pest ◽  
Aqueous Dispersion ◽  
Limiting Factors ◽  
Spray Dryer ◽  
Ionic Gelation ◽  
Biological Assays ◽  
Insect Pest Control

The fungus Beauveria bassiana is widely used for pest control; however, biostability and dispersion for broth pulverization are limiting factors for its application in the field. In this context, formulation techniques such as microencapsulation are viable alternatives. The aim of this work is to optimize B. bassiana formulations by spray dryer and evaluate its stability and biological activity against Spodoptera cosmioides compared to ionic gelatinization formulations. The fungus was biocompatible with all evaluated biopolymers (lignin, cellulose, starch, humic substances, and alginate). The encapsulation by spray drying was optimized by factorial design in an inlet and outlet air temperature of 120°C and 68°C, respectively; aspirator rate of 35 m3·h−1, feed flow rate of 12 mL·min−1; and drying gas flow at 35 L·h−1. The ionic gelation capsules were obtained using a 0.5% quantity of conidia in a 1% sodium alginate solution dropped into a 0.5 mol·L−1 CaCl2 solution using a peristaltic pump. Spray drying provided smaller microcapsules than those by ionic gelation. Both techniques produced more stable conidia when exposed to temperature and UV-radiation than non-formulated B. bassiana. The formulations prepared by spray drying showed gains at aqueous dispersion. Biological assays against Spodoptera cosmioides showed a mortality rate of up to 90%. These results demonstrate the suitability of encapsulating B. bassiana conidia stably in aqueous dispersion without loss of viability and virulence.

scholarly journals Cuticle degrading enzyme production by some isolates of the entomopathogenic fungus, Metarhizium anisopliae (Metsch.)

2014 ◽  
Vol 20 ◽  
pp. 25-32
Author(s):  
N Sapna Bai ◽  
OK Remadevi ◽  
TO Sasidharan ◽  
M Balachander ◽  
Priyadarsanan Dharmarajan
Keyword(s):  
Pest Control ◽  
Entomopathogenic Fungi ◽  
Enzyme Production ◽  
Insect Pest ◽  
Strong Relationship ◽  
Control Agent ◽  
Mechanical Pressure ◽  
Degrading Enzyme ◽  
Insect Pest Control ◽  
Degrading Enzymes

Context: Entomopathogenic fungi have been recognized as viable alternate options to chemicals in insect pest control. Unlike other potential biocontrol agents, fungi do not have to be ingested to infect their hosts but invade directly through the cuticle. Entry into the host involves both enzymic degradation of the cuticle barrier and mechanical pressure. Production of a range of cuticle degrading enzymes is an important event in the interaction of entomopathogenic fungi and host. Enzyme secretion is believed to be a key contributor for the virulence of a fungal isolate. Objectives: The potentiality of nine isolates of M. anisopliae were tested to produce to produce three important cuticle degrading enzymes, viz., chitinase, protease and lipase. Materials and Methods: Nine isolates of M. anisopliae were evaluated for chitinase, protease and lipase enzyme production by determining the enzyme index and activities. Results: Chitinase index of these isolates were ranged from 1.5 to 2.2 and chitinolytic activity from 0.525 to 1.560 U/ml. The isolates showed protease index in the range of 1.2 to 3.3 and the activity ranged from 0.020 to 0.114 U/ml. Lipase index ranged from 1.15 to 7.0 and the enzyme activity ranged from 0.153 to 0.500 U/ml. A strong relationship was observed between virulence of the isolates and cuticle degrading enzyme production as increased enzyme production was observed for virulent isolates. Conclusion: In the present study three isolates as (MIS2, MIS7 and MIS13) demonstrated cuticle degrading enzyme (CDE) that indicate higher virulence based on the bioassay conducted earlier by the authors as strongly substantiating the role of CDEs is considered the virulence of Metarhizium isolates. So, these isolates may be as ecofriendly insect-pest control agent in future. DOI: http://dx.doi.org/10.3329/jbs.v20i0.17648 J. bio-sci. 20: 25-32, 2012

Regulatory roles of microRNAs in insect pests: prospective targets for insect pest control

2021 ◽  
Vol 70 ◽  
pp. 158-166
Author(s):  
Qiang Zhang ◽  
Wei Dou ◽  
Clauvis Nji Tizi Taning ◽  
Guy Smagghe ◽  
Jin-Jun Wang
Keyword(s):  
Pest Control ◽  
Insect Pests ◽  
Insect Pest ◽  
Insect Pest Control

scholarly journals The Potential of Using Indigenous Pesticidal Plants for Insect Pest Control to Small Scale Farmers in Africa

2015 ◽  
Vol 06 (19) ◽  
pp. 3164-3174 ◽  
Author(s):  
Angela G. Mkindi ◽  
Kelvin M. Mtei ◽  
Karoli N. Njau ◽  
Patrick A. Ndakidemi
Keyword(s):  
Pest Control ◽  
Insect Pest ◽  
Small Scale ◽  
Insect Pest Control ◽  
Small Scale Farmers

scholarly journals Evaluation of New Systemic Insecticides for Elm Insect Pest Control

1996 ◽  
Vol 14 (1) ◽  
pp. 22-26 ◽  
Author(s):  
D. Casey Sclar ◽  
Whitney S. Cranshaw
Keyword(s):  
Pest Control ◽  
Woody Plants ◽  
Insect Pests ◽  
Insect Pest ◽  
Leaf Beetle ◽  
Pest Species ◽  
Shade Trees ◽  
Insect Pest Control ◽  
Systemic Activity ◽  
Systemic Insecticides

Abstract Use of systemic insecticides that can be injected either into the root system or trunk of woody plants provides several potential advantages, notably in control of drift during application. Recently, new classes of insecticides with systemic activity have been developed, which may supplant the organophosphate and carbamate systemic insecticides that have previously been available. To evaluate their potential to control insects affecting shade trees, studies were conducted using imidacloprid and abamectin on elm. Soil injections of imidacloprid appeared particularly effective, controlling all three of the target pest species in this study (elm leaf beetle, European elm scale, elm leaf aphid). Both imidacloprid and abamectin also were effective against at least some elm insects when injected into trunks. Persistence of irnidacloprid was unusually long, providing second season control of all elm insect pests, although root uptake following soil injections was slow.

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