An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
Keyword(s):
One Step
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We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.
2015 ◽
Vol 37
(2)
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pp. 221-229
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2013 ◽
Vol 25
(3)
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pp. 402-406
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2015 ◽
Vol 160
(10)
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pp. 2471-2477
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Keyword(s):
2016 ◽
Vol 237
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pp. 72-78
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2018 ◽
Vol 118
(12)
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pp. 772-776
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2004 ◽
Vol 16
(3)
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pp. 244-248
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