Corrigendum: Value of Pyruvate Carboxylase in Thyroid Fine-Needle Aspiration Wash-Out Fluid for Predicting Papillary Thyroid Cancer Lymph Node Metastasis

The incidence of papillary thyroid carcinoma (PTC) is increasing. Lymph node metastatic status of PTC is a major factor for decision marking of surgery and surgical extend, however, no reliable tool exists for prediction of PTC nodal metastasis, for example, ultrasound cannot qualitatively diagnose and effectively detect central lymph node metastasis (CLNM). Therefore, the development of a new diagnostic biomarker is crucial for CLNM. Metabolic dysregulation is an important factor associated with malignancy and metastasis of tumors. Pyruvate carboxylase (PC) is a major anaplerotic enzyme that catalyzes the carboxylation of pyruvate to form oxaloacetate, which has been suggested to be involved in the tumorigenesis of several cancers, including PTC. This study aimed to explore the role of PC expression in thyroid fine-needle aspiration (FNA) wash-out fluid for predicting CLNM in PTC, and to explore how PC is involved in PTC development. The expression levels of PC in PTC tissues and normal thyroid tissues were first compared based on bioinformatics analysis of public databases, including the Gene Expression Profiling (GEPIA), Oncomine and Gene Expression Omnibus (GEO) databases. Then, the PC mRNA and protein expression levels were measured by RT-PCR and Immunohistochemistry (IHC) in surgical tissues from a total of 42 patients with surgically confirmed PTC, and compared in patients with and without CLNM. Further, to assess PC expression in diagnostic biopsies, a total of 71 thyroid nodule patients with ultrasound-guided FNA wash-out fluid samples and cytological diagnosis were prospectively enrolled in the study. Then, we analyzed the mechanism of PC-mediated PTC progression in vitro. This study showed that PC expression was higher in PTC tissues and thyroid FNA wash-out fluid samples from patients with CLNM than those from patients without CLNM, and that PC-induced PTC metastasis may occur through the TGF-β/Smad-regulated epithelial–mesenchymal transition (EMT) pathway.

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Thyroglobulin Measurement in the Fine-Needle Aspiration Washout for Diagnosing Cervical Lymph Node Metastasis in the Patients with Differentiated Papillary Thyroid Cancer

Journal of the Korean Radiological Society ◽

10.3348/jkrs.2006.55.5.437 ◽

2006 ◽

Vol 55 (5) ◽

pp. 437

Author(s):

Byung Moon Kim ◽

Eun Kyung Kim ◽

Min Jung Kim

Keyword(s):

Thyroid Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Fine Needle Aspiration ◽

Cervical Lymph Node ◽

Cervical Lymph Node Metastasis ◽

Needle Aspiration ◽

Papillary Thyroid ◽

Fine Needle ◽

Node Metastasis

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Value of pyruvate carboxylase in thyroid fine-needle aspiration wash-out fluid for predicting PTC lymph node metastasis

10.21203/rs.3.rs-84129/v1 ◽

2020 ◽

Author(s):

Chang Liu ◽

Lu Zhang ◽

Yang Liu ◽

Qingqing Zhao ◽

Yu Pan ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Fine Needle Aspiration ◽

Needle Aspiration ◽

Nodal Metastasis ◽

Papillary Thyroid ◽

Fine Needle ◽

Node Metastasis ◽

Thyroid Fine Needle Aspiration ◽

Thyroid Fna

Abstract BackgroundThe incidence of papillary thyroid carcinoma (PTC) is increasing yearly. Knowing whether there is lymph node metastasis is of great value in choosing surgery and surgical methods, but ultrasound cannot qualitatively diagnose and effectively detect lymph node metastases in the central area. Metabolic dysregulation is an important factor associated with malignancy and metastasis of tumors. Pyruvate carboxylase (PC) is a major anaplerotic enzyme that catalyzes the carboxylation of pyruvate to form oxaloacetate (OAA) and is involved in tumorigenesis in several cancers. This study aims to explore the relationship between PC mRNA expression in thyroid fine-needle aspiration (FNA) wash-out fluid and lymph node metastasis of papillary thyroid carcinoma (PTC).MethodsThe expression levels of PC in PTCs and normal thyroid tissues were first compared based on bioinformatics analysis of public databases, including the Gene Expression Profiling (GEPIA), Oncomine and Gene Expression Omnibus (GEO) databases. Then, the PC mRNA levels were measured by RT-PCR in surgical tissues in a total of 42 patients with surgically confirmed PTC and compared to those of patients with and without central lymph nodal metastasis (CLNM). Further, to identify PC expression in diagnostic biopsies, a total of 71 thyroid nodule patients with ultrasound-guided fine-needle aspiration wash-out fluid samples and cytological diagnosis were prospectively enrolled in the study. ResultsThrough data mining, we found that PC is overexpressed in PTC and PTC with lymph node metastasis. In 42 surgically diagnosed PTC patient tumor tissues, PC was significantly higher in patients with CLNM (n=15) than in those without (n=27) nodal metastasis (median: 6.490 vs. 2.430, p = 0.014). Furthermore, in thyroid FNA wash-out fluid, PC was significantly higher in patients with cytologically diagnosed PTC (n=52) than in those with benign nodules (n =19) (median: 2.456 vs. 0.495, p = 0.005). Thirty-four of the 52 patients with PTC received a total thyroidectomy, and PC was also significantly higher in patients with CLNM (n=17) than in those without nodal metastasis (n=17) (median: 3.665 vs. 1.621, p = 0.013). The area under the ROC curve for PC predicting PTC with lymph node metastasis in FNA wash out samples was 0.751 (p = 0.013), and the sensitivity and specificity were 70.6% and 76.5%, respectively.ConclusionsThe findings of this study suggest that PC may predict malignancy in thyroid nodules and lymph nodal metastasis with thyroid FNA wash-out fluid in PTC patients.

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