scholarly journals Systemic Administration of Fibroblast Growth Factor 21 Improves the Recovery of Spinal Cord Injury (SCI) in Rats and Attenuates SCI-Induced Autophagy

2021 ◽  
Vol 11 ◽  
Author(s):  
Sipin Zhu ◽  
Yibo Ying ◽  
Lin Ye ◽  
Weiyang Ying ◽  
Jiahui Ye ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Functional Recovery ◽  
Nerve Cells ◽  
Systemic Administration ◽  
Fibroblast Growth Factor 21 ◽  
Fibroblast Growth ◽  
Cord Injury

Protecting the death of nerve cells is an essential tactic for spinal cord injury (SCI) repair. Recent studies show that nerve growth factors can reduce the death of nerve cells and promote the healing of nerve injury. To investigate the conducive effect of fibroblast growth factor 21 (FGF21) on SCI repair. FGF21 proteins were systemically delivered into rat model of SCI via tail vein injection. We found that administration of FGF21 significantly promoted the functional recovery of SCI as assessed by BBB scale and inclined plane test, and attenuated cell death in the injured area by histopathological examination with Nissl staining. This was accompanied with increased expression of NeuN, GAP43 and NF200, and deceased expression of GFAP. Interestingly, FGF21 was found to attenuate the elevated expression level of the autophagy marker LC3-II (microtubules associated protein 1 light chain 3-II) induced by SCI in a dose-dependent manner. These data show that FGF21 promotes the functional recovery of SCI via restraining injury-induced cell autophagy, suggesting that systemic administration of FGF21 could have a therapeutic potential for SCI repair.

Basic Fibroblast Growth Factor (bFGF) Enhances Functional Recovery Following Severe Spinal Cord Injury to the Rat

2000 ◽  
Vol 164 (2) ◽  
pp. 280-291 ◽  
Author(s):  
A.G. Rabchevsky ◽  
I. Fugaccia ◽  
A.F. Turner ◽  
D.A. Blades ◽  
M.P. Mattson ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Functional Recovery ◽  
Fibroblast Growth ◽  
Cord Injury

Basic Fibroblast Growth Factor (bFGF) Enhances Tissue Sparing and Functional Recovery Following Moderate Spinal Cord Injury

1999 ◽  
Vol 16 (9) ◽  
pp. 817-830 ◽  
Author(s):  
ALEXANDER G. RABCHEVSKY ◽  
ISABELLA FUGACCIA ◽  
ANITA FLETCHER-TURNER ◽  
DEBORAH A. BLADES ◽  
MARK P. MATTSON ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Functional Recovery ◽  
Fibroblast Growth ◽  
Cord Injury ◽  
Tissue Sparing

Increased Basic Fibroblast Growth Factor Expression Following Contusive Spinal Cord Injury

1996 ◽  
Vol 141 (1) ◽  
pp. 154-164 ◽  
Author(s):  
Italo Mocchetti ◽  
Stuart J. Rabin ◽  
Anna M. Colangelo ◽  
Scott R. Whittemore ◽  
Jean R. Wrathall
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Cord Injury ◽  
Factor Expression ◽  
Growth Factor Expression ◽  
Contusive Spinal Cord Injury

scholarly journals Gelatine nanostructured lipid carrier encapsulated FGF15 inhibits autophagy and improves recovery in spinal cord injury

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Yibo Ying ◽  
Guangheng Xiang ◽  
Min Chen ◽  
Jiahui Ye ◽  
Qiuji Wu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Immunofluorescent Staining ◽  
Therapeutic Effects ◽  
Nanostructured Lipid Carrier ◽  
Fibroblast Growth ◽  
Cord Injury ◽  
Associated Proteins

AbstractGelatine nanostructured lipid carriers (GNLs) have attracted increasing attention due to their biodegradable status and capacity to capture various biologically active compounds. Many studies demonstrated that fibroblast growth factor therapies after spinal cord injury (SCI) can be used in the future for the recovery of neurons. In this study, the therapeutic effects of GNL-encapsulated fibroblast growth factor 15 (FGF15) and FGF15 were compared in SCI. The FGF15-GNLs had 88.17 ± 1.22% encapsulation efficiency and 4.82 ± 0.12% loading capacity. The effects of FGF15-GNLs and FGF15 were assessed based on the Basso–Beattie–Bresnahan (BBB) locomotion scale, inclined plane test and footprint analysis. Immunofluorescent staining was used to identify the expression of autophagy-associated proteins, GFAP (glial fibrillary acidic protein) and neurofilament 200 (NF200). FGF15-GNLs use enhanced the repair after SCI compared to the effect of FGF15. The suppression of autophagy-associated proteins LC3-II and beclin-1, and p62 enhancement by FGF15-GNLs treatment were more pronounced. Thus, the effects of FGF15-GNLs on the recovery after SCI are related to the inhibition of autophagy and glial scar, and promotion of nerve regeneration in SCI.

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