Dissolving Polymer Microneedles for Transdermal Delivery of Insulin

It’s of great significance to develop insulin-loaded dissolving microneedles (MNs) which are fabricated with various methods and materials for transdermal delivery of insulin to effectively and efficiently treat diabetes. In this work, we present a kind of FITC-insulin tip-loaded dissolving MNs fabricated with the mixture of polyvinyl alcohol (PVA) and sucrose using homemade PDMS MNs mold under vacuum conditions. The uniform appearance of MN arrays contributes to controlling the drug dosage well as required. Sufficient mechanical strength for penetrating tough stratum corneum can be obtained by vacuum frozen-drying for at least 6 h after peeling MNs off the mold. About 90% of the FITC-insulin is localized in the conical MN tips and can be released into the skin within 2 min after insertion. The in vivo insulin absorption study and hypoglycemic effect in diabetic mice demonstrate that the proposed insulin-loaded MNs can efficiently deliver the insulin to the systemic circulation and exhibit a similar effect to hypodermic injection on hypoglycemic administration. Together these results suggested that the efficient MN fabrication process proposed in this work shows great potential for mass production and practical application of drug-loaded dissolving MNs in the future.

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Novel dissolving microneedles for enhanced transdermal delivery of levonorgestrel: In vitro and in vivo characterization

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2017.10.035 ◽

2017 ◽

Vol 534 (1-2) ◽

pp. 378-386 ◽

Cited By ~ 28

Author(s):

Gangtao Yao ◽

Guilan Quan ◽

Shiqi Lin ◽

Tingting Peng ◽

Qingqing Wang ◽

...

Keyword(s):

Transdermal Delivery ◽

Dissolving Microneedles ◽

In Vivo Characterization

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Mass Production and Characterization of Dissolving Microneedle Arrayed Patches for a Transdermal Delivery

10.26226/morressier.57ea3d6ad462b8028d88e1c4 ◽

2016 ◽

Author(s):

Hongkee Kim

Keyword(s):

Mass Production ◽

Transdermal Delivery

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Increased Circulatory Extrarenal 1α,25-Dihydroxyvitamin D3 in Bilaterally Nephrectomized Rats

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530320666200530222426 ◽

2020 ◽

Vol 20 (9) ◽

pp. 1523-1530

Author(s):

Murat Dabak ◽

Durrin O. Dabak ◽

Tuncay Kuloglu ◽

Ersoy Baydar ◽

Hakan Bulut ◽

...

Keyword(s):

Immune Cells ◽

Low Dose ◽

Systemic Circulation ◽

Bilateral Nephrectomy ◽

Dihydroxyvitamin D3 ◽

Hydroxyvitamin D ◽

Inflammatory Conditions ◽

Calcium Phosphorus ◽

25 Hydroxyvitamin D

Background: Extrarenal 1α,25-dihydroxyvitamin D3 (1,25-D) locally produced by immune cells plays crucial roles in the regulation of the immune system. However, in vivo status of extrarenal 1,25-D and 25-hydroxyvitamin D (25-D) in acute inflammatory conditions are unknown. Objective: The aim of this study was to determine the extrarenal 1,25-D level in circulation in bilaterally nephrectomized rats, induced by low-dose lipopolysaccharide (LPS). Methods: Renal 1,25-D synthesis was terminated through bilateral nephrectomy in rats. The rats received intraperitoneal LPS (50 μg/kg BW) three times and the experiment was ended 24 hours after nephrectomy. Serum 1,25-D, 25-D, calcium, phosphorus, intact parathyroid hormone, and calcitonin levels were measured and immunohistochemistry was applied to detect the sources of extrarenal 1,25- D synthesis. Results: Circulatory 1,25-D concentration remarkably increased in both LPS-treated and non-treated bilaterally nephrectomized rats. Elevated circulatory 1,25-D did not have hypercalcemic endocrinal effects. The increased 1,25-D level also resulted in a concurrent rapid and dramatic depletion of circulatory 25-D. Conclusions: Extrarenal 1,25-D could enter into the systemic circulation and, therefore, might have systemic effects besides its autocrine and paracrine functions.

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In Vivo Digestion of Egg Products Enriched with DHA: Effect of the Food Matrix on DHA Bioavailability

Foods ◽

10.3390/foods10010006 ◽

2020 ◽

Vol 10 (1) ◽

pp. 6

Author(s):

Carlos Pineda-Vadillo ◽

Françoise Nau ◽

Catherine Guérin-Dubiard ◽

Claire Bourlieu ◽

Francesco Capozzi ◽

...

Keyword(s):

Docosahexaenoic Acid ◽

Venous Catheter ◽

Systemic Circulation ◽

Food Matrix ◽

Postprandial Period ◽

Egg Products ◽

Efficient Vector

The aim of the present study was to determine to what extent the food matrix could affect the release of docosahexaenoic acid (DHA) during digestion and its incorporation into systemic circulation. In this aim, three DHA-enriched egg products having the same composition but different structure were developed: omelet, hard-boiled egg, and mousse. Then, nine pigs fitted with T-shape cannulas at duodenal level and a jugular venous catheter were fed with the DHA-enriched egg products, and duodenal effluents and plasma were collected throughout the postprandial period. Results highlighted an undeniable effect of the food matrix on digestion parameters and DHA bioavailability. The transit of DHA and protein through the duodenum was faster after the ingestion of the mousse than after the ingestion of the omelet and hard-boiled egg. While most of the DHA and protein ingested under the form of mousse had already passed through the duodenum 4.5 h after its ingestion, significantly higher quantities were still present in the case of the omelet and hard-boiled egg. In terms of bioavailability, the omelet was the most efficient vector for delivering DHA into systemic circulation. It supplied 56% and 120% more DHA than the hard-boiled egg and the mousse, respectively.

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In vitro and in vivo evaluation of Bola-surfactant containing niosomes for transdermal delivery

Biomedical Microdevices ◽

10.1007/s10544-007-9046-6 ◽

2007 ◽

Vol 9 (4) ◽

pp. 421-433 ◽

Cited By ~ 47

Author(s):

Donatella Paolino ◽

Rita Muzzalupo ◽

Antonio Ricciardi ◽

Christian Celia ◽

Nevio Picci ◽

...

Keyword(s):

Transdermal Delivery ◽

In Vivo Evaluation ◽

Bola Surfactant

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Formulation, characterization and in vitro–in vivo evaluation of flurbiprofen-loaded nanostructured lipid carriers for transdermal delivery

Drug Development and Industrial Pharmacy ◽

10.3109/03639045.2012.686509 ◽

2012 ◽

Vol 39 (4) ◽

pp. 569-578 ◽

Cited By ~ 39

Author(s):

Jitendra Kawadkar ◽

Ashwinkumar Pathak ◽

Raj Kishore ◽

Meenakshi Kanwar Chauhan

Keyword(s):

Transdermal Delivery ◽

Nanostructured Lipid Carriers ◽

In Vivo Evaluation

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Ophthalmic Drug Dosage Forms: Characterisation and Research Methods

The Scientific World JOURNAL ◽

10.1155/2014/861904 ◽

2014 ◽

Vol 2014 ◽

pp. 1-14 ◽

Cited By ~ 87

Author(s):

Przemysław Baranowski ◽

Bożena Karolewicz ◽

Maciej Gajda ◽

Janusz Pluta

Keyword(s):

Defense Mechanisms ◽

Drug Application ◽

Drug Dosage ◽

Eye Drops ◽

Eye Tissues ◽

Ophthalmic Drug ◽

Application Frequency

This paper describes hitherto developed drug forms for topical ocular administration, that is, eye drops, ointments,in situgels, inserts, multicompartment drug delivery systems, and ophthalmic drug forms with bioadhesive properties. Heretofore, many studies have demonstrated that new and more complex ophthalmic drug forms exhibit advantage over traditional ones and are able to increase the bioavailability of the active substance by, among others, reducing the susceptibility of drug forms to defense mechanisms of the human eye, extending contact time of drug with the cornea, increasing the penetration through the complex anatomical structure of the eye, and providing controlled release of drugs into the eye tissues, which allows reducing the drug application frequency. The rest of the paper describes recommendedin vitroandin vivostudies to be performed for various ophthalmic drugs forms in order to assess whether the form is acceptable from the perspective of desired properties and patient’s compliance.

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Transdermal Delivery of Scopolamine by Natural Submicron Injectors: In-Vivo Study in Pig

PLoS ONE ◽

10.1371/journal.pone.0031922 ◽

2012 ◽

Vol 7 (2) ◽

pp. e31922 ◽

Cited By ~ 11

Author(s):

Esther Shaoul ◽

Ari Ayalon ◽

Yossi Tal ◽

Tamar Lotan

Keyword(s):

Transdermal Delivery ◽

In Vivo Study

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SECRETION OF PROGESTERONE DURING GESTATION IN THE RAT

Journal of Endocrinology ◽

10.1677/joe.0.0790179 ◽

1978 ◽

Vol 79 (2) ◽

pp. 179-190 ◽

Cited By ~ 37

Author(s):

MRINAL K. SANYAL

Keyword(s):

Abdominal Aorta ◽

Primary Source ◽

Solvent System ◽

Peripheral Circulation ◽

Systemic Circulation ◽

Maternal Circulation ◽

Secondary Importance ◽

System Hexane ◽

Venous Plasma

The concentrations of progesterone and 5α-pregnane-3,20-dione in ovarian and uterine venous plasma and in the systemic circulation were measured during gestation in the rat. The steroids were quantified by radioimmunoassay after separation on silicic acid microcolumns with the solvent system hexane: ethyl acetate (5: 2, v/v). The concentration of progesterone in the systemic circulation was highest on days 3–4 and 13–17 of pregnancy; throughout gestation, the concentration of 5α-pregnane-3,20-dione was low in relation to that of progesterone and showed no marked changes as gestation proceeded. The level of progesterone in ovarian venous effluent was 10–20 times higher than that in the uterine vein and 20–50 times greater than that in the systemic circulation. The rate of secretion of progesterone by the ovary was highest during days 13–17 of gestation and ovariectomy during this period markedly reduced the levels of progesterone in the peripheral circulation. The concentration of progesterone in the uterine venous effluent was raised compared with the concentration in plasma from the abdominal aorta, especially on days 7 and 9 of pregnancy. These results suggest that, in vivo, the rat placenta synthesizes small amounts of progesterone and secretes it into the maternal circulation. The ovary is the primary source of progesterone during pregnancy and the placental contribution is of secondary importance. Although 4-ene-5α-reductase enzyme(s) is present in the ovary and placenta, significant quantities of the reduced progestin 5α-pregnane-3,20-dione are not secreted into the systemic circulation during gestation in the rat.

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