In vitro shoot proliferation and in vitro and ex vitro root formation of Pyrus elaeagrifolia Pallas

The growth and development of lingonberry (Vaccinium vitis-idaea L.) plants propagated either by conventional softwood cuttings or by in vitro shoot proliferation from nodal explants and by shoot regeneration from excised leaves of micropropagated shoots, were studied in cultivars `Regal', `Splendor', and `Erntedank'. Significant differences were observed between the treatments. After 3 years of growth, the in vitro-derived plants produced more stems, leaves, and rhizomes than the conventional cuttings which rarely produced rhizomes. In vitro culture on nutrient medium apparently induces the juvenile branching characteristics that favor rhizome production. This increase in vegetative growth and rhizome yield of in vitro-derived plants over stem cuttings varied among genotypes.

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Pyroligneous Acid Improves In Vitro Rooting of Japanese Pear Cultivars

HortScience ◽

10.21273/hortsci.37.1.194 ◽

2002 ◽

Vol 37 (1) ◽

pp. 194-195 ◽

Cited By ~ 5

Author(s):

Masanori Kadota ◽

Takashi Hirano ◽

Kiyotoshi Imizu ◽

Yoshiji Niimi

Keyword(s):

Root Formation ◽

Shoot Proliferation ◽

Shoot Multiplication ◽

In Vitro Rooting ◽

Japanese Pear ◽

Pyrus Pyrifolia ◽

Shoot Cultures ◽

Pyroligneous Acid ◽

In Vitro Shoot Proliferation

Effects of PA on in vitro shoot proliferation and root formation were investigated using shoot cultures of three Japanese pear (Pyrus pyrifolia Nakai) cultivars. PA inhibited shoot multiplication and promoted initiation and development of roots in the cultured shoots of three cultivars, resulting in increasing the proportion of rooted shoots. Chemical name used: pyroligneous acid (PA).

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Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

Revista Colombiana de Ciencias Hortícolas ◽

10.17584/rcch.2018v12i2.7226 ◽

2018 ◽

Vol 12 (2) ◽

pp. 405-415

Author(s):

Paulo Mauricio Centenaro Bueno ◽

Luiz Antonio Biasi ◽

Mauro Brasil Dias Tofanelli

Keyword(s):

Culture Medium ◽

Shoot Proliferation ◽

Ex Vitro Rooting ◽

Ms Medium ◽

Ex Vitro ◽

Indolebutyric Acid ◽

Simple Protocol ◽

Growth Room ◽

In Vitro Shoot Proliferation

This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.

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Growth and Development of Lingonberry Cultivars as Affected by In Vitro and Ex Vitro Culture Methods and Source Propagule

HortScience ◽

10.21273/hortsci.39.4.891a ◽

2004 ◽

Vol 39 (4) ◽

pp. 891A-891 ◽

Cited By ~ 2

Author(s):

Samir Debnath*

Keyword(s):

Growth And Development ◽

Shoot Proliferation ◽

Lower Number ◽

Morphological Development ◽

Multiplication Rate ◽

Stem Cuttings ◽

Ex Vitro ◽

Culture Methods ◽

In Vitro Shoot Proliferation

The morphological development of lingonberry (Vaccinium vitis-idaea L.) plants propagated either by conventional softwood cuttings or by in vitro shoot proliferation from nodal explants or by shoot regeneration from excised leaves of micropropagated shoots, was studied in cultivars `Regal', `Splendor', and `Erntedank'. Significant differences were observed between the treatments. In vitro-derived plants produced more shoots branches and rhizomes in contrast to conventional cuttings which rarely produced rhizomes. Plants propagated from cuttings had a lower number but vigorous shoots and thicker rhizomes than in vitro-derived plants. Source propagule had significant effect on multiplication rate. Another experiment evaluated the effect of indole-3-butyric acid (IBA) application to softwood cuttings on subsequent rooting, shoot development, and rhizome production. Treating cuttings with IBA did not significantly improve rhizome formation and elongation. In vitro culture on nutrient medium apparently induces the juvenile branching characteristics that favored rhizome production. The advantage of rhizome production of in vitro-derived plants over stem cuttings varied among genotypes.

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Factors Affecting Ex vitro Rooting in Micropropagated Shoots from Nodal Explants of Terminalia arjuna

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2020/v39i4131123 ◽

2020 ◽

pp. 92-97

Author(s):

Meena Choudhary ◽

Inder Dev Arya ◽

Sarita Arya

Keyword(s):

Large Scale ◽

Incubation Temperature ◽

Shoot Proliferation ◽

Ex Vitro Rooting ◽

Terminalia Arjuna ◽

Ex Vitro ◽

Important Species ◽

Factors Affecting ◽

In Vitro Shoot Proliferation

The present work was done with the aim to study the effect of rooting mixture and incubation temperature on Ex vitro rooting of Terminalia arjuna, an important multipurpose tree. The nodal explant collected from Ummaid garden Jodhpur was subjected for In vitro shoot proliferation on BAP supplemented modified MS medium. These shoots were In vitro multiplied on BAP (half concentration of BAP used in In vitro shoot proliferation) with low concentration of NAA supplemented medium. The individual shoots from In vitro multiplied shoots were pulse treated with IBA for 10 min. and transferred in different rooting mixture and incubation temperature for Ex vitro rooting. Analysis of data revealed that maximum 62.22% rooting was observed when the plantlet pulse treated with 984.25 µM IBA for 10 min were transferred on bottle containing vermiculite as rooting mixture and incubated at the temperature of 26°C.The optimization of Ex vitro rooting mixture and temperature conditions will be helpful in propagation of this important species rapidly in large scale.

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Micropropagation of Vitis vinifera L. cv. ‘Malagouzia’ and ‘Xinomavro’

Open Life Sciences ◽

10.2478/s11535-010-0073-6 ◽

2010 ◽

Vol 5 (6) ◽

pp. 839-852 ◽

Cited By ~ 3

Author(s):

Fotini Skiada ◽

Katerina Grigoriadou ◽

Eleftherios Eleftheriou

Keyword(s):

Vitis Vinifera ◽

Growth Regulators ◽

Root Formation ◽

Shoot Proliferation ◽

Shoot Development ◽

Vitis Vinifera L ◽

Higher Temperature ◽

Basal Media ◽

In Vitro Shoot Proliferation

AbstractThe effects of six basal media on in vitro shoot proliferation of the greek grapevines Vitis vinifera L. cv. ‘Malagouzia’ and ‘Xinomavro’ were investigated. Galzy and Zlenco proved to be the most effective for ‘Malagouzia’ and ‘Xinomavro’, respectively. If only BA was present in the medium, shoot development was poor and the plantlets were chlorotic. When the medium was supplemented with BA and NAA, growth was enhanced. The best ratio (in μM) of growth regulators was 0.5/0.3 for ‘Malagouzia’, and 0.1/0.03 for ‘Xinomavro’, which resulted in the highest number of microshoots per explant and greatest proliferation rate. The development of ‘Malagouzia’ and ‘Xinomavro’ explants at 21±2 and 26±2°C was also investigated, revealing the higher temperature to be more effective. Regarding rooting, 0.5 μM IBA improved root formation at 26°C for ‘Malagouzia’ and 0.5 μM IBA at 21°C for ‘Xinomavro’. Moreover, 0.5 μM IBA resulted in a higher rooting percentage (>95%) and proved to be more beneficial for the overall morphological appearance of the plantlets of ‘Malagouzia’. After acclimatization, survival of microshoots cultivated in media with IBA was higher than those in NAA.

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