scholarly journals Growth and Development of Lingonberry Cultivars as Affected by In Vitro and Ex Vitro Culture Methods and Source Propagule

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 891A-891 ◽  
Author(s):  
Samir Debnath*
Keyword(s):  
Growth And Development ◽  
Shoot Proliferation ◽  
Lower Number ◽  
Morphological Development ◽  
Multiplication Rate ◽  
Stem Cuttings ◽  
Ex Vitro ◽  
Culture Methods ◽  
In Vitro Shoot Proliferation

The morphological development of lingonberry (Vaccinium vitis-idaea L.) plants propagated either by conventional softwood cuttings or by in vitro shoot proliferation from nodal explants or by shoot regeneration from excised leaves of micropropagated shoots, was studied in cultivars `Regal', `Splendor', and `Erntedank'. Significant differences were observed between the treatments. In vitro-derived plants produced more shoots branches and rhizomes in contrast to conventional cuttings which rarely produced rhizomes. Plants propagated from cuttings had a lower number but vigorous shoots and thicker rhizomes than in vitro-derived plants. Source propagule had significant effect on multiplication rate. Another experiment evaluated the effect of indole-3-butyric acid (IBA) application to softwood cuttings on subsequent rooting, shoot development, and rhizome production. Treating cuttings with IBA did not significantly improve rhizome formation and elongation. In vitro culture on nutrient medium apparently induces the juvenile branching characteristics that favored rhizome production. The advantage of rhizome production of in vitro-derived plants over stem cuttings varied among genotypes.

scholarly journals Morphological Development of Lingonberry as Affected by In Vitro and Ex Vitro Propagation Methods and Source Propagule

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 760-763 ◽  
Author(s):  
Samir C. Debnath
Keyword(s):  
Vegetative Growth ◽  
Nutrient Medium ◽  
Growth And Development ◽  
Shoot Proliferation ◽  
Morphological Development ◽  
Stem Cuttings ◽  
Ex Vitro ◽  
Propagation Methods ◽  
In Vitro Shoot Proliferation

The growth and development of lingonberry (Vaccinium vitis-idaea L.) plants propagated either by conventional softwood cuttings or by in vitro shoot proliferation from nodal explants and by shoot regeneration from excised leaves of micropropagated shoots, were studied in cultivars `Regal', `Splendor', and `Erntedank'. Significant differences were observed between the treatments. After 3 years of growth, the in vitro-derived plants produced more stems, leaves, and rhizomes than the conventional cuttings which rarely produced rhizomes. In vitro culture on nutrient medium apparently induces the juvenile branching characteristics that favor rhizome production. This increase in vegetative growth and rhizome yield of in vitro-derived plants over stem cuttings varied among genotypes.

High in vitro shoot proliferation in the apple cultivar Jonagold induced by benzyladenine analogues

2002 ◽  
Vol 50 (2) ◽  
pp. 191-195 ◽  
Author(s):  
K. Magyar-Tábori ◽  
J. Dobránszki ◽  
E. Jámbor-Benczúr
Keyword(s):  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Length ◽  
Multiplication Rate ◽  
Apple Cultivar ◽  
High Concentration ◽  
Suppression Effect ◽  
High Concentrations ◽  
In Vitro Shoot Proliferation

The in vitro shoot multiplication of apple cv. Jonagold was tested on media containing benzyladenine, benzyladenine riboside or meta-topolin in different concentrations (from 0.0 to 5.0 mg l-1). The optimal concentration for the best multiplication varied according to the type of cytokinin. The highest multiplication rate (on average 6.9 and 5.9 new shoots per explant) was achieved using 5.0 mg l-1 meta-topolin or 2.0 mg l-1 benzyladenine riboside. The longest shoots were formed on media containing benzyladenine riboside at a concentration of 0.5 mg l-1. The length of newly developed shoots was strongly suppressed by high concentrations of different cytokinins, but the suppression effect of a high concentration of meta-topolin on shoot length was less than that of benzyladenine or benzyladenine riboside. In this study meta-topolin and benzyladenine riboside proved to be effective cytokinins to induce adequate shoot proliferation, while benzyladenine was the least active cytokinin

scholarly journals Effect of different levels of NAA on in vitro growth and development of shoots of Dendrobium orchid

1970 ◽  
Vol 34 (3) ◽  
pp. 411-416 ◽  
Author(s):  
MS Parvin ◽  
ME Haque ◽  
F Akhter ◽  
M Moniruzzaman ◽  
ABM Khaldun
Keyword(s):  
Growth And Development ◽  
Shoot Proliferation ◽  
Shoot Length ◽  
In Vitro Growth ◽  
Shoot Number ◽  
Number Of Leaves ◽  
Shoot Weight ◽  
Different Levels ◽  
In Vitro Shoot Proliferation

The present study was conducted to investigate the effect of growth regulator NAA on in vitro shoot proliferation, rooting, and plantlet establishment. Among the different concentrations of NAA, the best increase in shoot weight (0.25 g) and shoot number (8.83) were observed from 0.1 mg/I NAA. The highest shoot length (2.60 cm), number of leaves (4.83), number of roots (5.15), and root length (2.67 cm) were obtained with 0.2 mg/I NAA at 60 DAT. Key Words: Dendrobium orchid, NAA, MS media. DOI: 10.3329/bjar.v34i3.3966 Bangladesh J. Agril. Res. 34(3) : 411-416, September 2009

scholarly journals The Effect of TIBA and NPA on Shoot Regeneration of Cannabis sativa L. Epicotyl Explants

Agronomy ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 104
Author(s):  
Mariola Dreger ◽  
Milena Szalata
Keyword(s):  
Shoot Regeneration ◽  
Apical Dominance ◽  
Large Scale ◽  
Cannabis Sativa ◽  
Shoot Proliferation ◽  
Multiplication Rate ◽  
Culture Methods ◽  
Specific Chemical ◽  
Industrial Hemp

Industrial hemp (Cannabis sativa L., family Cannabaceae) is a multi-purpose crop, used in the production of food, nutraceuticals, cosmetics and medicines. Therefore, development of new varieties with specific chemical profiles is necessary. In vitro culture methods could be complementary to conventional breeding and a useful tool for large-scale propagation. Strong apical dominance is considered as one of the factors contributing to the recalcitrance of industrial hemp in shoot proliferation. In this study, we tested the polar transport inhibitors N-1-naphtylphtalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) to enhance shoot regeneration as the result of suppression of apical dominance and to develop in vitro protocols for Diana, Finola and Fedora 17 cultivars. Shoot tips derived from epicotyls were cultivated on Murashige and Skoog medium (MS) supplemented with meta-topolin (mT) and NPA, and also thidiazuron (TDZ) with a combination of TIBA and NPA. The results showed that the combination of TDZ with NPA (1–5 mg L−1) and TDZ with TIBA (0.5–2.5 mg L−1) increased the response of explants and the multiplication rate, but the effect was genotype-dependent and malformations were observed. To optimize the developed protocol, a two-step procedure with shortened time of exposure to inhibitors and reduced concentrations of them was applied. Shoots were rooted on media containing indole-3-butyric acid (IBA) and then successfully acclimatized. The obtained results will be useful in micropropagation of recalcitrant industrial hemp varieties.

scholarly journals Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

2018 ◽  
Vol 12 (2) ◽  
pp. 405-415
Author(s):  
Paulo Mauricio Centenaro Bueno ◽  
Luiz Antonio Biasi ◽  
Mauro Brasil Dias Tofanelli
Keyword(s):  
Culture Medium ◽  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Ms Medium ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Simple Protocol ◽  
Growth Room ◽  
In Vitro Shoot Proliferation

This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.

scholarly journals Factors Affecting Ex vitro Rooting in Micropropagated Shoots from Nodal Explants of Terminalia arjuna

2020 ◽  
pp. 92-97
Author(s):  
Meena Choudhary ◽  
Inder Dev Arya ◽  
Sarita Arya
Keyword(s):  
Large Scale ◽  
Incubation Temperature ◽  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Terminalia Arjuna ◽  
Ex Vitro ◽  
Important Species ◽  
Factors Affecting ◽  
In Vitro Shoot Proliferation

The present work was done with the aim to study the effect of rooting mixture and incubation temperature on Ex vitro rooting of Terminalia arjuna, an important multipurpose tree. The nodal explant collected from Ummaid garden Jodhpur was subjected for In vitro shoot proliferation on BAP supplemented modified MS medium. These shoots were In vitro multiplied on BAP (half concentration of BAP used in In vitro shoot proliferation) with low concentration of NAA supplemented medium. The individual shoots from In vitro multiplied shoots were pulse treated with IBA for 10 min. and transferred in different rooting mixture and incubation temperature for Ex vitro rooting. Analysis of data revealed that maximum 62.22% rooting was observed when the plantlet pulse treated with 984.25 µM IBA for 10 min were transferred on bottle containing vermiculite as rooting mixture and incubated at the temperature of 26°C.The optimization of Ex vitro rooting mixture and temperature conditions will be helpful in propagation of this important species rapidly in large scale.

scholarly journals Micropropagation of Lingonberry: Influence of Genotype, Explant Orientation, and Overcoming TDZ-induced Inhibition of Shoot Elongation Using Zeatin

HortScience ◽  
2005 ◽  
Vol 40 (1) ◽  
pp. 185-188 ◽  
Author(s):  
Samir C. Debnath
Keyword(s):  
Shoot Proliferation ◽  
Shoot Elongation ◽  
Axillary Shoot ◽  
Multiplication Rate ◽  
Shoot Height ◽  
Explant Orientation ◽  
Low Concentrations ◽  
Shoot Number ◽  
In Vitro Shoot Proliferation

In an attempt to improve the micropropagation protocol for lingonberry (Vaccinium vitis-idaea L.) developed at the Centre, two lingonberry clones were compared for in vitro shoot proliferation on two different media supplemented with varying levels of thidiazuron (TDZ). TDZ supported proliferation at low concentrations (0.1 to 1 μm) but inhibited shoot elongation. However, usable shoots were obtained within 4 weeks by transferring shoot cluster to medium containing 1 μm zeatin. Genotypes differed significantly with respect to multiplication rate with `EL1' producing the most shoots per explant. In both genotypes, shoot proliferation was greatly influenced by explant orientation. Changing the orientation of explants from vertically upright to horizontal increased axillary shoot number, but decreased shoot height and leaf number per shoot. Proliferated shoots were rooted on a 2 peat: 1 perlite (v/v) medium, and the plantlets were acclimatized and eventually established in the greenhouse.

scholarly journals Neem Oil Used as a “Complex Mixture” to Improve In Vitro Shoot Proliferation in Olive

HortScience ◽  
2018 ◽  
Vol 53 (4) ◽  
pp. 531-534 ◽  
Author(s):  
Maurizio Micheli ◽  
Daniel Fernandes da Silva ◽  
Daniela Farinelli ◽  
Graziana Agate ◽  
Rafael Pio ◽  
...  
Keyword(s):  
Complex Mixture ◽  
Shoot Proliferation ◽  
Multiplication Rate ◽  
Neem Oil ◽  
In Vitro Plant Regeneration ◽  
Olive Cultivar ◽  
Proliferation Medium ◽  
Aseptic Conditions ◽  
In Vitro Shoot Proliferation

Shoots of the olive cultivar Moraiolo were previously cultured in aseptic conditions on Olive Medium (OM), with the addition of 4 mg·L−1 of zeatin, 30 g·L−1 of sucrose, and 7 g·L−1 of agar. Then, 1-cm long uninodal explants with two leaves and two axillary buds were excised from the proliferated masses and placed on the same proliferation medium enriched with four concentrations of neem oil (0—control, 0.1, 0.5, and 1.0 mL·L−1), added before sterilization. The addition of 0.1 mL·L−1 of neem oil to the medium gave an improvement in shoot regeneration. More vigorous shoots (longer proliferated shoots) were obtained along with a higher number of nodes (multiplication rate). Overall, there was a significant increase in the total fresh and dry proliferated weights. To our knowledge, this is the first report showing a strong and beneficial effect of neem oil, used as a “complex mixture,” on in vitro plant regeneration.

scholarly journals The elaboration of a practical protocol for the micropropagation of several apple rootstock varieties

2016 ◽  
Vol 73 (2) ◽  
pp. 226
Author(s):  
Doina Clapa ◽  
Alexandru Fira ◽  
Manuela Simu ◽  
Monica Harta ◽  
Cristian Sisea
Keyword(s):  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Wheat Starch ◽  
Axillary Shoot ◽  
Multiplication Rate ◽  
Ex Vitro ◽  
Apple Rootstock ◽  
Axillary Shoot Proliferation ◽  
D 20

The apple rootstock varieties ‘MM 106/4’, ‘MM 106/6’, ‘D 18’, ‘D 20’, ‘JTE-H’ and ‘MR 09/4’ were multiplied in vitro on modified Murashige and Skoog media gelled with wheat starch (MSs) and supplemented with 0.7 mg/l BA, which provided intense axillary shoot proliferation. Among the genotypes we studied, MR 09/4 had the highest multiplication rate  (19.56), followed by D18 (15.36). The lowest multiplication rates were recorded in MM 106/6 (5.36) and in MM 106/4 (3.32). The use of the technique of direct ex vitro rooting and acclimatization in floating perlite provided rooting percentages of more than 90 %.

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