Integration of Physical, Genetic, and Cytogenetic Mapping Data for Cellulose Synthase (CesA) Genes in Flax (Linum usitatissimum L.)

Abstract In this work we used publicly available raw RNA-seq data to elucidate mechanisms of flax fiber biogenesis by measuring expression of cell-wall related genes (cellulose synthase, cellulose synthase-like and chitinase-like genes) in stem of flax (Linum usitatissimum cv. Bethune). Using public RNA-sequence data we have quantified and characterised the expression of the specific cell-wall genes in the top, middle and bottom parts of the Bethune flax stem. The most prominent findings are: Secondary cell-wall cellulose synthase (CesA) genes are expressed differentially in phloem and xylem in all parts of Bethune stem, in contrast with primary cell-wall cellulose synthase genes. Total expression level of cellulose synthase-like (Csl) genes is tissue invariant (although, CslG and CslE are differentially expressed) and smaller than the total expression of cellulose synthase genes. The CslD2D3 subgroup dominates total expression of CslD genes in both xylem and phloem. Expression levels of all expressed chitinase-like (Ctl) genes are tissue dependent in all parts of stem. Total expression level of chitinase-like genes is much higher than expression of cellulose synthase and cellulose synthase-like genes in both tissues.

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Comparative Transcriptomic Analysis Identifies Key Cellulose Synthase Genes (CESA) and Cellulose Synthase-like Genes (CSL) in Fast Growth Period of Flax Stem (Linum Usitatissimum L.)

Journal of Natural Fibers ◽

10.1080/15440478.2021.1993510 ◽

2021 ◽

pp. 1-16

Author(s):

Yuan Guo ◽

Lan Wen ◽

Jikang Chen ◽

Gen Pan ◽

Zhimin Wu ◽

...

Keyword(s):

Linum Usitatissimum ◽

Growth Period ◽

Cellulose Synthase ◽

Fast Growth ◽

Transcriptomic Analysis ◽

Linum Usitatissimum L ◽

Comparative Transcriptomic Analysis

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Cell wall gene expression in two sub-species of flax

The EuroBiotech Journal ◽

10.24190/issn2564-615x/2017/02.15 ◽

2017 ◽

Vol 1 (2) ◽

pp. 192-195 ◽

Cited By ~ 1

Author(s):

Dmitry Galinousky ◽

Tsimafei Padvitski

Keyword(s):

Gene Expression ◽

Cell Wall ◽

Linum Usitatissimum ◽

Fiber Quality ◽

Cellulose Synthase ◽

Expression Level ◽

Fiber Flax ◽

Linum Usitatissimum L ◽

Lower Expression ◽

Dehiscent Flax

Abstract In this work, we attempted to elucidate mechanisms of flax fiber biogenesis by measuring, using qPCR, expression of the cellwall related genes (cellulose synthase, cellulose synthase-like and chitinase-like genes) in stems of two contrasting fiber quality subspecies of flax (Linum usitatissimum L.). We studied elongatum Vav. et Ell. (cultivar Blakit, Belarus) and crepitans Boenn. (dehiscent flax) subspecies, which are differed in the height of plants, width of stems etc. Amongst all measured genes chitinase- like Ctl19 and Ctl21 genes showed drastic difference in expression between stems of the two flax varieties, showing higher expression level in the fiber flax versus the dehiscent flax. In contrast, cellulose synthase-like CslG4 gene had lower expression levels in the stem of fiber flax than in dehiscent flax. We suggest that hemicellulose composition and cellulose - non-cellulose glycan organisation can vary between stalk cells of different flax subspecies.

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