QTL Mapping and Validation of Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Humai 15

Abstract Background: Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant varieties are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions, in addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under controlled greenhouse conditions.Results: Seventeen accessions showed stable high-level resistance to stripe rust across all environments under field tests, while four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers covering the whole genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99%–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, while four and one QTL conferring seedling and adult-plant resistance respectively were mapped distantly from previously reported stripe rust resistance genes or QTL and may be novel resistance loci.Conclusions: Our results provided an integrated view of stripe rust resistance resources in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.

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Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone

10.21203/rs.3.rs-22210/v2 ◽

2020 ◽

Author(s):

Yuqi Wang ◽

Can Yu ◽

Yukun Cheng ◽

Fangjie Yao ◽

Li Long ◽

...

Keyword(s):

Stripe Rust ◽

Plant Resistance ◽

Rust Resistance ◽

Adult Plant Resistance ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Wheat Landrace ◽

Landrace Diversity ◽

Chinese Wheat ◽

Rust Resistance Genes

Abstract Background: Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant varieties are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions, in addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under controlled greenhouse conditions. Results: Seventeen accessions showed stable high-level resistance to stripe rust across all environments under field tests, while four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers covering the whole genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99%–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, while four and one QTL conferring seedling and adult-plant resistance respectively were mapped distantly from previously reported stripe rust resistance genes or QTL and may be novel resistance loci. Conclusions: Our results provided an integrated view of stripe rust resistance resources in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.

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Molecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai

Frontiers in Plant Science ◽

10.3389/fpls.2021.756557 ◽

2021 ◽

Vol 12 ◽

Author(s):

Yuqi Wang ◽

Fengying Liang ◽

Fangnian Guan ◽

Fangjie Yao ◽

Li Long ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Stripe Rust ◽

Plant Resistance ◽

Quantitative Trait ◽

Adult Plant Resistance ◽

Puccinia Striiformis ◽

Adult Plant ◽

Wheat Landrace ◽

Trait Loci ◽

Chinese Wheat

The Chinese wheat landrace “Gaoxianguangtoumai” (GX) has exhibited a high level of adult-plant resistance (APR) to stripe rust in the field for more than a decade. To reveal the genetic background for APR to stripe rust in GX, a set of 249 F6:8 (F6, F7, and F8) recombinant inbred lines (RILs) was developed from a cross between GX and the susceptible cultivar “Taichung 29.” The parents and RILs were evaluated for disease severity at the adult-plant stage in the field by artificial inoculation with the currently predominant Chinese Puccinia striiformis f. sp. tritici races during three cropping seasons and genotyped using the Wheat 55K single-nucleotide polymorphism (SNP) array to construct a genetic map with 1,871 SNP markers finally. Two stable APR quantitative trait loci (QTL), QYr.GX-2AS and QYr.GX-7DS in GX, were detected on chromosomes 2AS and 7DS, which explained 15.5–27.0% and 11.5–13.5% of the total phenotypic variation, respectively. Compared with published Yr genes and QTL, QYr.GX-7DS and Yr18 may be the same, whereas QYr.GX-2AS is likely to be novel. Haplotype analysis revealed that QYr.GX-2AS is likely to be rare which presents in 5.3% of the 325 surveyed Chinese wheat landraces. By analyzing a heterogeneous inbred family (HIF) population from a residual heterozygous plant in an F8 generation of RIL, QYr.GX-2AS was further flanked by KP2A_36.85 and KP2A_38.22 with a physical distance of about 1.37Mb and co-segregated with the KP2A_37.09. Furthermore, three tightly linked Kompetitive allele-specific PCR (KASP) markers were highly polymorphic among 109 Chinese wheat cultivars. The results of this study can be used in wheat breeding for improving resistance to stripe rust.

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Identification of QTL for adult plant resistance to stripe rust in Chinese wheat landrace Caoxuan 5

Euphytica ◽

10.1007/s10681-014-1349-0 ◽

2015 ◽

Vol 204 (3) ◽

pp. 627-634 ◽

Cited By ~ 4

Author(s):

X. L. Zhou ◽

Y. Zhang ◽

Q. D. Zeng ◽

X. M. Chen ◽

D. J. Han ◽

...

Keyword(s):

Stripe Rust ◽

Plant Resistance ◽

Adult Plant Resistance ◽

Adult Plant ◽

Wheat Landrace ◽

Chinese Wheat

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QTL mapping of adult-plant resistance to leaf rust based on SSR markers and SNP sequencing of Chinese wheat landrace Xu’ai (Triticum aestivum L.)

Genetic Resources and Crop Evolution ◽

10.1007/s10722-020-01067-3 ◽

2021 ◽

Author(s):

Xinyu Xu ◽

Zhenying Duan ◽

jihua Su ◽

Xing Li ◽

Jizhong Wu ◽

...

Keyword(s):

Triticum Aestivum ◽

Qtl Mapping ◽

Leaf Rust ◽

Ssr Markers ◽

Plant Resistance ◽

Adult Plant Resistance ◽

Triticum Aestivum L ◽

Adult Plant ◽

Wheat Landrace ◽

Chinese Wheat

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QTL Mapping of Adult-Plant Resistance to Stripe Rust in Chinese Wheat Cultivar Chuanyu 16

Journal of Agricultural Science ◽

10.5539/jas.v4n3p57 ◽

2011 ◽

Vol 4 (3) ◽

Author(s):

Youliang Zheng ◽

Ling Wu ◽

Xianchun Xia ◽

Yunliang Peng ◽

Huazhong Zhu ◽

...

Keyword(s):

Qtl Mapping ◽

Stripe Rust ◽

Plant Resistance ◽

Adult Plant Resistance ◽

Wheat Cultivar ◽

Adult Plant ◽

Chinese Wheat Cultivar ◽

Chinese Wheat

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Mendelization and Molecular Mapping of a Large-effect QTL Conferring Durable Adult-plant Resistance to Stripe Rust in a Chinese Wheat Landrace ‘Gaoxianguangtoumai’

10.21203/rs.3.rs-603248/v1 ◽

2021 ◽

Author(s):

Yuqi Wang ◽

Fengying Liang ◽

Fangnian Guan ◽

Fangjie Yao ◽

Long Li ◽

...

Keyword(s):

Stripe Rust ◽

Plant Resistance ◽

Adult Plant Resistance ◽

Puccinia Striiformis ◽

Snp Array ◽

Adult Plant ◽

Isogenic Line ◽

Wheat Landrace ◽

Chinese Wheat ◽

Kasp Markers

Abstract The Chinese wheat landrace ‘Gaoxianguangtoumai’ (GX) has exhibited a high degree of adult-plant resistance (APR) to stripe rust in field environments for more than a decade. To reveal the genetic basis for APR to stripe rust in GX, a set of 249 F6:8 recombinant inbred lines (RILs) was developed from a cross between GX and the susceptible cultivar ‘Taichung 29’. The parents and RILs were evaluated for disease severity at the adult-plant stage in field environments by artificial inoculation with the currently predominant Chinese Puccinia striiformis f. sp. tritici races during three cropping seasons, and genotyped using the Wheat 55K single-nucleotide polymorphism (SNP) array to construct a genetic map with 1,871 SNP markers. Two stable APR quantitative trait loci (QTL), QYr.GX-2AS and QYr.GX-7DS from GX, were detected on chromosomes 2AS and 7DS, which explained 15.5–27.0% and 9.6–15.6% of the total phenotypic variation, respectively. Compared with published genes and QTL, QYr.GX-7DS is likely Yr18, whereas QYr.GX-2AS is probably novel. Haplotype analysis revealed that QYr.GX-2AS is likely to be rare which present in 5.3% of the 325 surveyed Chinese wheat landraces. By analyzing a near-isogenic line population, QYr.GX-2AS was further mapped to an interval with a physical distance of about 1.37 Mb and co-segregated with a Kompetitive allele-specific PCR (KASP) marker. Furthermore, three tightly linked KASP markers were highly polymorphic among 109 Chinese wheat cultivars. The short physical interval and tightly linked KASP markers developed in this study will facilitate marker-assisted selection and map-based cloning of QYr.GX-2AS.

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Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone

10.21203/rs.3.rs-22210/v4 ◽

2020 ◽

Author(s):

Yuqi Wang ◽

Can Yu ◽

Yukun Cheng ◽

Fangjie Yao ◽

Li Long ◽

...

Keyword(s):

Stripe Rust ◽

Plant Resistance ◽

Rust Resistance ◽

Adult Plant Resistance ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Wheat Landrace ◽

Landrace Diversity ◽

Chinese Wheat ◽

Rust Resistance Genes

Abstract Background: Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant cultivars are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions. In addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under a controlled greenhouse environment.Results: Seventeen accessions showed stable high-level resistance to stripe rust across all environments in the field tests. Four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers that covered the entire genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99%–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, whereas four and one QTL conferring seedling and adult-plant resistance, respectively, were mapped distantly from previously reported stripe rust resistance genes or QTL and thus may be novel resistance loci.Conclusions: Our results provided an integrated overview of stripe rust resistance resources in a wheat landrace diversity panel from the southern autumn-sown spring wheat zone of China. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.

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Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone

BMC Genomics ◽

10.1186/s12864-020-07331-1 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Yuqi Wang ◽

Can Yu ◽

Yukun Cheng ◽

Fangjie Yao ◽

Li Long ◽

...

Keyword(s):

Stripe Rust ◽

Plant Resistance ◽

Rust Resistance ◽

Adult Plant Resistance ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Wheat Landrace ◽

Landrace Diversity ◽

Chinese Wheat ◽

Rust Resistance Genes

Abstract Background Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant cultivars are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions. In addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under a controlled greenhouse environment. Results Seventeen accessions showed stable high-level resistance to stripe rust across all environments in the field tests. Four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers that covered the entire genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, whereas four and one QTL conferring seedling and adult-plant resistance, respectively, were mapped distantly from previously reported stripe rust resistance genes or QTL and thus may be novel resistance loci. Conclusions Our results provided an integrated overview of stripe rust resistance resources in a wheat landrace diversity panel from the southern autumn-sown spring wheat zone of China. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.

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