scholarly journals Molecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuqi Wang ◽  
Fengying Liang ◽  
Fangnian Guan ◽  
Fangjie Yao ◽  
Li Long ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Quantitative Trait ◽  
Adult Plant Resistance ◽  
Puccinia Striiformis ◽  
Adult Plant ◽  
Wheat Landrace ◽  
Trait Loci ◽  
Chinese Wheat

The Chinese wheat landrace “Gaoxianguangtoumai” (GX) has exhibited a high level of adult-plant resistance (APR) to stripe rust in the field for more than a decade. To reveal the genetic background for APR to stripe rust in GX, a set of 249 F6:8 (F6, F7, and F8) recombinant inbred lines (RILs) was developed from a cross between GX and the susceptible cultivar “Taichung 29.” The parents and RILs were evaluated for disease severity at the adult-plant stage in the field by artificial inoculation with the currently predominant Chinese Puccinia striiformis f. sp. tritici races during three cropping seasons and genotyped using the Wheat 55K single-nucleotide polymorphism (SNP) array to construct a genetic map with 1,871 SNP markers finally. Two stable APR quantitative trait loci (QTL), QYr.GX-2AS and QYr.GX-7DS in GX, were detected on chromosomes 2AS and 7DS, which explained 15.5–27.0% and 11.5–13.5% of the total phenotypic variation, respectively. Compared with published Yr genes and QTL, QYr.GX-7DS and Yr18 may be the same, whereas QYr.GX-2AS is likely to be novel. Haplotype analysis revealed that QYr.GX-2AS is likely to be rare which presents in 5.3% of the 325 surveyed Chinese wheat landraces. By analyzing a heterogeneous inbred family (HIF) population from a residual heterozygous plant in an F8 generation of RIL, QYr.GX-2AS was further flanked by KP2A_36.85 and KP2A_38.22 with a physical distance of about 1.37Mb and co-segregated with the KP2A_37.09. Furthermore, three tightly linked Kompetitive allele-specific PCR (KASP) markers were highly polymorphic among 109 Chinese wheat cultivars. The results of this study can be used in wheat breeding for improving resistance to stripe rust.

scholarly journals Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410

2020 ◽  
Vol 21 (2) ◽  
pp. 478 ◽  
Author(s):  
Yan Liu ◽  
Yanmin Qie ◽  
Xing Li ◽  
Meinan Wang ◽  
Xianming Chen
Keyword(s):  
High Temperature ◽  
Quantitative Trait Loci ◽  
Stripe Rust ◽  
Spring Wheat ◽  
Quantitative Trait ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Landrace ◽  
Trait Loci

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat in the world. Genetic resistance is the best strategy for control of the disease. Spring wheat landrace PI 181410 has shown high level resistance to stripe rust. The present study characterized the landrace to have both race-specific all-stage resistance and nonrace-specific high-temperature adult-plant (HTAP) resistance. To map quantitative trait loci (QTL) for the resistance in PI 181410, it was crossed with Avocet S (AvS), from which a recombinant inbred line population was developed. The F5–F8 populations were consecutively phenotyped for stripe rust response in multiple field environments under natural Pst infection, and the F7 population was phenotyped in seedlings at low temperature and in adult-plant stage with selected Pst races in the greenhouse. The F7 population was genotyped using the 90K wheat SNP chip. Three QTL, QYrPI181410.wgp-4AS, QYrPI181410.wgp-4BL, and QYrPI181410.wgp-5BL.1, from PI 181410 for all-stage resistance, were mapped on chromosome arms 4AS, 4BL, and 5BL, respectively. Four QTL, QYrPI181410.wgp-1BL, QYrPI181410.wgp-4BL, QYrPI181410.wgp-5AS, and QYrPI181410.wgp-5BL.2, were identified from PI 181410 for HTAP resistance and mapped to 1BL, 4BL, 5AS, and 5BL, respectively. Two QTL with minor effects on stripe rust response were identified from AvS and mapped to 2BS and 2BL. Four of the QTL from PI 181410 and one from AvS were potentially new. As the 4BL QTL was most effective and likely a new gene for stripe rust resistance, three kompetitive allele specific PCR (KASP) markers were developed for incorporating this gene into new wheat cultivars.

scholarly journals Mendelization and Molecular Mapping of a Large-effect QTL Conferring Durable Adult-plant Resistance to Stripe Rust in a Chinese Wheat Landrace ‘Gaoxianguangtoumai’

2021 ◽  
Author(s):  
Yuqi Wang ◽  
Fengying Liang ◽  
Fangnian Guan ◽  
Fangjie Yao ◽  
Long Li ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Plant Resistance ◽  
Adult Plant Resistance ◽  
Puccinia Striiformis ◽  
Snp Array ◽  
Adult Plant ◽  
Isogenic Line ◽  
Wheat Landrace ◽  
Chinese Wheat ◽  
Kasp Markers

Abstract The Chinese wheat landrace ‘Gaoxianguangtoumai’ (GX) has exhibited a high degree of adult-plant resistance (APR) to stripe rust in field environments for more than a decade. To reveal the genetic basis for APR to stripe rust in GX, a set of 249 F6:8 recombinant inbred lines (RILs) was developed from a cross between GX and the susceptible cultivar ‘Taichung 29’. The parents and RILs were evaluated for disease severity at the adult-plant stage in field environments by artificial inoculation with the currently predominant Chinese Puccinia striiformis f. sp. tritici races during three cropping seasons, and genotyped using the Wheat 55K single-nucleotide polymorphism (SNP) array to construct a genetic map with 1,871 SNP markers. Two stable APR quantitative trait loci (QTL), QYr.GX-2AS and QYr.GX-7DS from GX, were detected on chromosomes 2AS and 7DS, which explained 15.5–27.0% and 9.6–15.6% of the total phenotypic variation, respectively. Compared with published genes and QTL, QYr.GX-7DS is likely Yr18, whereas QYr.GX-2AS is probably novel. Haplotype analysis revealed that QYr.GX-2AS is likely to be rare which present in 5.3% of the 325 surveyed Chinese wheat landraces. By analyzing a near-isogenic line population, QYr.GX-2AS was further mapped to an interval with a physical distance of about 1.37 Mb and co-segregated with a Kompetitive allele-specific PCR (KASP) marker. Furthermore, three tightly linked KASP markers were highly polymorphic among 109 Chinese wheat cultivars. The short physical interval and tightly linked KASP markers developed in this study will facilitate marker-assisted selection and map-based cloning of QYr.GX-2AS.

scholarly journals Quantitative Trait Loci Mapping for Adult-Plant Resistance to Powdery Mildew in Bread Wheat

2006 ◽  
Vol 96 (7) ◽  
pp. 784-789 ◽  
Author(s):  
S. S. Liang ◽  
K. Suenaga ◽  
Z. H. He ◽  
Z. L. Wang ◽  
H. Y. Liu ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Powdery Mildew ◽  
Plant Resistance ◽  
Quantitative Trait ◽  
Powdery Mildew Resistance ◽  
Adult Plant Resistance ◽  
Adult Plant ◽  
Phenotypic Variance ◽  
Mildew Resistance ◽  
Trait Loci

Powdery mildew, caused by Blumeria graminis f. sp. tritici, is a major disease to wheat (Triticum aestivum) worldwide. Use of adult-plant resistance (APR) is an effective method to develop wheat cultivars with durable resistance to powdery mildew. In the present study, 432 molecular markers were used to map quantitative trait loci (QTL) for APR to powdery mildew in a doubled haploid (DH) population with 107 lines derived from the cross Fukuho-komugi × Oligoculm. Field trials were conducted in Beijing and Anyang, China during 2003-2004 and 2004-2005 cropping seasons, respectively. The DH lines were planted in a randomized complete block design with three replicates. Artificial inoculation was carried out in Beijing with highly virulent isolate E20 of B. graminis f. sp. tritici and the powdery mildew severity on penultimate leaf was evaluated four times, and the maximum disease severity (MDS) on penultimate leaf was investigated in Anyang under natural inoculation in May 2004 and 2005. The heritability of resistance to powdery mildew for MDS in 2 years and two locations ranged from 0.82 to 0.93, while the heritability for area under the disease progress curve was between 0.84 and 0.91. With the method of composite interval mapping, four QTL for APR to powdery mildew were detected on chromosomes 1AS, 2BL, 4BL, and 7DS, explaining 5.7 to 26.6% of the phenotypic variance. Three QTL on chromosomes 1AS, 2BL, and 7DS were derived from the female, Fukuho-komugi, while the one on chromosome 4BL was from the male, Oligoculm. The QTL on chromosome 1AS showed high genetic effect on powdery mildew resistance, accounting for 19.5 to 26.6% of phenotypic variance across two environments. The QTL on 7DS associated with the locus Lr34/Yr18, flanked by microsatellite Xgwm295.1 and Ltn (leaf tip necrosis). These results will benefit for improving powdery mildew resistance in wheat breeding programs.

scholarly journals Quantitative Trait Loci for Adult-Plant Resistance to Mycosphaerella graminicola in Two Winter Wheat Populations

2011 ◽  
Vol 101 (10) ◽  
pp. 1209-1216 ◽  
Author(s):  
P. Risser ◽  
E. Ebmeyer ◽  
V. Korzun ◽  
L. Hartl ◽  
T. Miedaner
Keyword(s):  
Quantitative Trait Loci ◽  
Winter Wheat ◽  
Plant Height ◽  
Plant Resistance ◽  
Quantitative Trait ◽  
Adult Plant Resistance ◽  
Heading Date ◽  
Adult Plant ◽  
Phenotypic Variance ◽  
Trait Loci

Septoria tritici blotch (STB) is one of the most important leaf spot diseases in wheat worldwide. The goal of this study was to detect chromosomal regions for adult-plant resistance in large winter wheat populations to STB. Inoculation by two isolates with virulence to Stb6 and Stb15, both present in the parents, was performed and STB severity was visually scored plotwise as percent coverage of flag leaves with pycnidia-bearing lesions. ‘Florett’/‘Biscay’ and ‘Tuareg’/‘Biscay’, each comprising a cross of a resistant and a susceptible cultivar, with population sizes of 316 and 269 F7:8 recombinant inbred lines, respectively, were phenotyped across four and five environments and mapped with amplified fragment length polymorphism, diversity array technology, and simple sequence repeat markers covering polymorphic regions of ≈1,340 centimorgans. Phenotypic data revealed significant (P < 0.01) genotypic differentiation for STB, heading date, and plant height. Entry-mean heritabilities (h2) for STB were 0.73 for ‘Florett’/‘Biscay’ and 0.38 for ‘Tuareg’/‘Biscay’. All correlations between STB and heading date as well as between STB and plant height were low (r = –0.13 to –0.20). In quantitative trait loci (QTL) analysis, nine and six QTL were found for STB ratings explaining, together, 55 and 51% of phenotypic variation in ‘Florett’/‘Biscay’ and ‘Tuareg’/‘Biscay’, respectively. Genotype–environment and QTL–environment interactions had a large impact. Two major QTL were detected consistently across environments on chromosomes 3B and 6D from ‘Florett’ and chromosomes 4B and 6B from ‘Tuareg’, each explaining 12 to 17% of normalized adjusted phenotypic variance. These results indicate that adult-plant resistance to STB in both mapping populations was of a quantitative nature.

scholarly journals Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone

2020 ◽  
Author(s):  
Yuqi Wang ◽  
Can Yu ◽  
Yukun Cheng ◽  
Fangjie Yao ◽  
Li Long ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Plant Resistance ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Landrace ◽  
Landrace Diversity ◽  
Chinese Wheat ◽  
Rust Resistance Genes

Abstract Background: Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant varieties are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions, in addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under controlled greenhouse conditions.Results: Seventeen accessions showed stable high-level resistance to stripe rust across all environments under field tests, while four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers covering the whole genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99%–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, while four and one QTL conferring seedling and adult-plant resistance respectively were mapped distantly from previously reported stripe rust resistance genes or QTL and may be novel resistance loci.Conclusions: Our results provided an integrated view of stripe rust resistance resources in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.

scholarly journals Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone

2020 ◽  
Author(s):  
Yuqi Wang ◽  
Can Yu ◽  
Yukun Cheng ◽  
Fangjie Yao ◽  
Li Long ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Plant Resistance ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Landrace ◽  
Landrace Diversity ◽  
Chinese Wheat ◽  
Rust Resistance Genes

Abstract Background: Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant varieties are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions, in addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under controlled greenhouse conditions. Results: Seventeen accessions showed stable high-level resistance to stripe rust across all environments under field tests, while four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers covering the whole genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99%–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, while four and one QTL conferring seedling and adult-plant resistance respectively were mapped distantly from previously reported stripe rust resistance genes or QTL and may be novel resistance loci. Conclusions: Our results provided an integrated view of stripe rust resistance resources in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.

Molecular mapping of quantitative trait loci for adult-plant resistance to powdery mildew in Italian wheat cultivar Libellula

2012 ◽  
Vol 63 (6) ◽  
pp. 539 ◽  
Author(s):  
M. A. Asad ◽  
B. Bai ◽  
C. X. Lan ◽  
J. Yan ◽  
X. C. Xia ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Powdery Mildew ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Wheat Cultivar ◽  
Stripe Rust Resistance ◽  
Adult Plant

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a fungal disease that causes significant yield losses in many wheat-growing regions of the world. Previously, five quantitative trait loci (QTLs) for adult-plant resistance (APR) to stripe rust resistance were identified in Italian wheat cultivar Libellula. The objectives of this study were to map QTLs for APR to powdery mildew in 244 F2 : 3 lines of Libellula/Huixianhong, to analyse the stability of detected QTLs across environments, and to assess the association of these QTLs with stripe rust resistance. Powdery mildew response was evaluated for 2 years in Beijing and for 1 year in Anyang. The correlation between averaged maximum disease severity (MDS) and averaged area under disease progress curve (AUDPC) over 2 years in Beijing was 0.98, and heritabilities of MDS and AUDPC were 0.65 and 0.81, respectively, based on the mean values averaged across environments. SSR markers were used to screen the parents and mapping population. Five QTLs were identified by inclusive composite interval mapping, designated as QPm.caas-2DS, QPm.caas-4BL.1, QPm.caas-6BL.1, QPm.caas-6BL.2, and QPm.caas-7DS. Three QTLs (QPm.caas-2DS and QPm.caas-6BL.1, and QPm.caas-6BL.2) seem to be new resistance loci for powdery mildew. QTLs QPm.caas-2DS and QPm.caas-4BL.1 were identified at the same position as previously mapped QTLs for stripe rust resistance in Libellula. The QTL QPm.caas-7DS, derived from Libellula, coincided with the slow rusting and slow mildewing locus Lr34/Yr18/Pm38. These results and the identified markers could be useful for wheat breeders aiming for durable resistance to both powdery mildew and stripe rust.

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