Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat in the world. Genetic resistance is the best strategy for control of the disease. Spring wheat landrace PI 181410 has shown high level resistance to stripe rust. The present study characterized the landrace to have both race-specific all-stage resistance and nonrace-specific high-temperature adult-plant (HTAP) resistance. To map quantitative trait loci (QTL) for the resistance in PI 181410, it was crossed with Avocet S (AvS), from which a recombinant inbred line population was developed. The F5–F8 populations were consecutively phenotyped for stripe rust response in multiple field environments under natural Pst infection, and the F7 population was phenotyped in seedlings at low temperature and in adult-plant stage with selected Pst races in the greenhouse. The F7 population was genotyped using the 90K wheat SNP chip. Three QTL, QYrPI181410.wgp-4AS, QYrPI181410.wgp-4BL, and QYrPI181410.wgp-5BL.1, from PI 181410 for all-stage resistance, were mapped on chromosome arms 4AS, 4BL, and 5BL, respectively. Four QTL, QYrPI181410.wgp-1BL, QYrPI181410.wgp-4BL, QYrPI181410.wgp-5AS, and QYrPI181410.wgp-5BL.2, were identified from PI 181410 for HTAP resistance and mapped to 1BL, 4BL, 5AS, and 5BL, respectively. Two QTL with minor effects on stripe rust response were identified from AvS and mapped to 2BS and 2BL. Four of the QTL from PI 181410 and one from AvS were potentially new. As the 4BL QTL was most effective and likely a new gene for stripe rust resistance, three kompetitive allele specific PCR (KASP) markers were developed for incorporating this gene into new wheat cultivars.

Download Full-text

Molecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai

Frontiers in Plant Science ◽

10.3389/fpls.2021.756557 ◽

2021 ◽

Vol 12 ◽

Author(s):

Yuqi Wang ◽

Fengying Liang ◽

Fangnian Guan ◽

Fangjie Yao ◽

Li Long ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Stripe Rust ◽

Plant Resistance ◽

Quantitative Trait ◽

Adult Plant Resistance ◽

Puccinia Striiformis ◽

Adult Plant ◽

Wheat Landrace ◽

Trait Loci ◽

Chinese Wheat

The Chinese wheat landrace “Gaoxianguangtoumai” (GX) has exhibited a high level of adult-plant resistance (APR) to stripe rust in the field for more than a decade. To reveal the genetic background for APR to stripe rust in GX, a set of 249 F6:8 (F6, F7, and F8) recombinant inbred lines (RILs) was developed from a cross between GX and the susceptible cultivar “Taichung 29.” The parents and RILs were evaluated for disease severity at the adult-plant stage in the field by artificial inoculation with the currently predominant Chinese Puccinia striiformis f. sp. tritici races during three cropping seasons and genotyped using the Wheat 55K single-nucleotide polymorphism (SNP) array to construct a genetic map with 1,871 SNP markers finally. Two stable APR quantitative trait loci (QTL), QYr.GX-2AS and QYr.GX-7DS in GX, were detected on chromosomes 2AS and 7DS, which explained 15.5–27.0% and 11.5–13.5% of the total phenotypic variation, respectively. Compared with published Yr genes and QTL, QYr.GX-7DS and Yr18 may be the same, whereas QYr.GX-2AS is likely to be novel. Haplotype analysis revealed that QYr.GX-2AS is likely to be rare which presents in 5.3% of the 325 surveyed Chinese wheat landraces. By analyzing a heterogeneous inbred family (HIF) population from a residual heterozygous plant in an F8 generation of RIL, QYr.GX-2AS was further flanked by KP2A_36.85 and KP2A_38.22 with a physical distance of about 1.37Mb and co-segregated with the KP2A_37.09. Furthermore, three tightly linked Kompetitive allele-specific PCR (KASP) markers were highly polymorphic among 109 Chinese wheat cultivars. The results of this study can be used in wheat breeding for improving resistance to stripe rust.

Download Full-text

Quantitative trait loci for non-race-specific, high-temperature adult-plant resistance to stripe rust in wheat cultivar Express

Theoretical and Applied Genetics ◽

10.1007/s00122-008-0894-0 ◽

2008 ◽

Vol 118 (4) ◽

pp. 631-642 ◽

Cited By ~ 68

Author(s):

F. Lin ◽

X. M. Chen

Keyword(s):

High Temperature ◽

Quantitative Trait Loci ◽

Stripe Rust ◽

Plant Resistance ◽

Quantitative Trait ◽

Adult Plant Resistance ◽

Wheat Cultivar ◽

Adult Plant ◽

Trait Loci

Download Full-text

Quantitative trait loci of stripe rust resistance in wheat

Theoretical and Applied Genetics ◽

10.1007/s00122-013-2159-9 ◽

2013 ◽

Vol 126 (10) ◽

pp. 2427-2449 ◽

Cited By ~ 111

Author(s):

G. M. Rosewarne ◽

S. A. Herrera-Foessel ◽

R. P. Singh ◽

J. Huerta-Espino ◽

C. X. Lan ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Stripe Rust ◽

Quantitative Trait ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Trait Loci

Download Full-text

Mapping Quantitative Trait Loci for High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat PI 197734 Using a Doubled Haploid Population and Genotyping by Multiplexed Sequencing

Frontiers in Plant Science ◽

10.3389/fpls.2020.596962 ◽

2020 ◽

Vol 11 ◽

Author(s):

Lu Liu ◽

Congying Yuan ◽

Meinan Wang ◽

Deven R. See ◽

Xianming Chen

Keyword(s):

High Temperature ◽

Quantitative Trait Loci ◽

Spring Wheat ◽

Plant Resistance ◽

Doubled Haploid ◽

Quantitative Trait ◽

Adult Plant Resistance ◽

Adult Plant ◽

Doubled Haploid Population ◽

Haploid Population

Download Full-text

Quantitative Trait Loci for High-Temperature Adult-Plant and Slow-Rusting Resistance to Puccinia striiformis f. sp. tritici in Wheat Cultivars

Phytopathology ◽

10.1094/phyto-98-7-0803 ◽

2008 ◽

Vol 98 (7) ◽

pp. 803-809 ◽

Cited By ~ 43

Author(s):

Q. Guo ◽

Z. J. Zhang ◽

Y. B. Xu ◽

G. H. Li ◽

J. Feng ◽

...

Keyword(s):

High Temperature ◽

Stripe Rust ◽

Phenotypic Variation ◽

Quantitative Trait ◽

Puccinia Striiformis ◽

Infection Type ◽

Adult Plant ◽

Wheat Cultivars ◽

Slow Rusting ◽

Adult Plants

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most damaging diseases of wheat (Triticum aestivum) globally. High-temperature adult-plant resistance (HTAPR) and slow-rusting have great potential for sustainable management of the disease. The wheat cultivars Luke and Aquileja have been previously reported to possess HTAPR and slow-rusting to stripe rust, respectively. Aquileja displayed less number of stripes per unit leaf area than Luke, while Luke showed lower infection type than Aquileja at adult-plant stages of growth under high-temperature conditions. The objectives of this study were to confirm the resistances and to map the resistance genes in Luke and Aquileja. Luke was crossed with Aquileja, and 326 of the F2 plants were genotyped using 282 microsatellite primer pairs. These F2 plants and their derived F3 families were evaluated for resistance to stripe rust by inoculation in the fields and greenhouses of high- and low-temperatures. Infection type was recorded for both seedlings and adult plants, and stripe number was recorded for adult plants only. Two quantitative trait loci (QTL) were identified, on the short arm of chromosome 2B, to be significantly associated with infection type at adult-plant stages in the fields and in the high-temperature greenhouse. The locus distal to centromere, referred to as QYrlu.cau-2BS1, and the locus proximal to centromere, referred to as QYrlu.cau-2BS2, were separated by a genetic distance of about 23 cM. QYrlu.cau-2BS1 was flanked by the microsatellite markers Xwmc154 and Xgwm148, and QYrlu.cau-2BS2 was flanked by Xgwm148 and Xabrc167. QYrlu.cau-2BS1 and QYrlu.cau-2BS2 explained up to 36.6 and 41.5% of the phenotypic variation of infection type, respectively, and up to 78.1% collectively. No significant interaction between the two loci was detected. Another QTL, referred to as QYraq.cau-2BL, was detected on the long arm of chromosome 2B to be significantly associated with stripe number. QYraq.cau-2BL was flanked by the microsatellite markers Xwmc175 and Xwmc332, and it explained up to 61.5% of the phenotypic variation of stripe number. It is possible that these three QTL are previously unmapped loci for resistance to stripe rust.

Download Full-text

High-temperature adult-plant resistance to stripe rust in facultative winter wheat

Crop and Pasture Science ◽

10.1071/cp16073 ◽

2016 ◽

Vol 67 (10) ◽

pp. 1064 ◽

Cited By ~ 2

Author(s):

Beyhan Akin ◽

Xian Ming Chen ◽

Alex Morgunov ◽

Nusret Zencirci ◽

Anmin Wan ◽

...

Keyword(s):

Molecular Markers ◽

High Temperature ◽

Winter Wheat ◽

Stripe Rust ◽

Field Experiments ◽

Puccinia Striiformis ◽

Yellow Rust ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Improvement Program

Stripe (yellow) rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss., is one of the most damaging diseases in wheat and is especially damaging for winter and facultative wheat. The objective of this study was to understand stripe rust resistance in 100 wheat and facultative wheat entries from the International Winter Wheat Improvement Program by conducting experiments in a greenhouse and in four field environments in Washington State, USA, and by genotyping molecular markers linked to Yr genes. Percentages of entries resistant to the rust races at the seedling stage were: PST-17, 44%; PST-37, 32%; PST-43, 45%; PST-45, 49%; PST-116, 18%; PST-100, 17%; and PST-127, 8%. Molecular markers were positive for genes Yr9, Yr17, and Yr18 and negative for Yr5, Yr10, and Yr15. Yr18 was present in 44 entries (44%). By using the highly virulent races PST-127 and PST-100 under controlled conditions, 16 entries were shown to have high-temperature adult-plant (HTAP) resistance and resistant–moderately resistant field reactions at all four field sites. Resistant entries, especially those with HTAP resistance, were also identified in the field experiments.

Download Full-text

Quantitative Trait Loci Mapping of Adult Plant and Seedling Resistance to Stripe Rust (Puccinia striiformis Westend.) in a Multiparent Advanced Generation Intercross Wheat Population

Frontiers in Plant Science ◽

10.3389/fpls.2021.684671 ◽

2021 ◽

Vol 12 ◽

Author(s):

Sandra Rollar ◽

Manuel Geyer ◽

Lorenz Hartl ◽

Volker Mohler ◽

Frank Ordon ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Quantitative Trait ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Seedling Resistance ◽

Wheat Population ◽

Advanced Generation

Stripe rust caused by the biotrophic fungus Puccinia striiformis Westend. is one of the most important diseases of wheat worldwide, causing high yield and quality losses. Growing resistant cultivars is the most efficient way to control stripe rust, both economically and ecologically. Known resistance genes are already present in numerous cultivars worldwide. However, their effectiveness is limited to certain races within a rust population and the emergence of stripe rust races being virulent against common resistance genes forces the demand for new sources of resistance. Multiparent advanced generation intercross (MAGIC) populations have proven to be a powerful tool to carry out genetic studies on economically important traits. In this study, interval mapping was performed to map quantitative trait loci (QTL) for stripe rust resistance in the Bavarian MAGIC wheat population, comprising 394 F6 : 8 recombinant inbred lines (RILs). Phenotypic evaluation of the RILs was carried out for adult plant resistance in field trials at three locations across three years and for seedling resistance in a growth chamber. In total, 21 QTL for stripe rust resistance corresponding to 13 distinct chromosomal regions were detected, of which two may represent putatively new QTL located on wheat chromosomes 3D and 7D.

Download Full-text

Molecular mapping of quantitative trait loci for adult-plant resistance to powdery mildew in Italian wheat cultivar Libellula

Crop and Pasture Science ◽

10.1071/cp12174 ◽

2012 ◽

Vol 63 (6) ◽

pp. 539 ◽

Cited By ~ 9

Author(s):

M. A. Asad ◽

B. Bai ◽

C. X. Lan ◽

J. Yan ◽

X. C. Xia ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Powdery Mildew ◽

Stripe Rust ◽

Plant Resistance ◽

Quantitative Trait ◽

Rust Resistance ◽

Adult Plant Resistance ◽

Wheat Cultivar ◽

Stripe Rust Resistance ◽

Adult Plant

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a fungal disease that causes significant yield losses in many wheat-growing regions of the world. Previously, five quantitative trait loci (QTLs) for adult-plant resistance (APR) to stripe rust resistance were identified in Italian wheat cultivar Libellula. The objectives of this study were to map QTLs for APR to powdery mildew in 244 F2 : 3 lines of Libellula/Huixianhong, to analyse the stability of detected QTLs across environments, and to assess the association of these QTLs with stripe rust resistance. Powdery mildew response was evaluated for 2 years in Beijing and for 1 year in Anyang. The correlation between averaged maximum disease severity (MDS) and averaged area under disease progress curve (AUDPC) over 2 years in Beijing was 0.98, and heritabilities of MDS and AUDPC were 0.65 and 0.81, respectively, based on the mean values averaged across environments. SSR markers were used to screen the parents and mapping population. Five QTLs were identified by inclusive composite interval mapping, designated as QPm.caas-2DS, QPm.caas-4BL.1, QPm.caas-6BL.1, QPm.caas-6BL.2, and QPm.caas-7DS. Three QTLs (QPm.caas-2DS and QPm.caas-6BL.1, and QPm.caas-6BL.2) seem to be new resistance loci for powdery mildew. QTLs QPm.caas-2DS and QPm.caas-4BL.1 were identified at the same position as previously mapped QTLs for stripe rust resistance in Libellula. The QTL QPm.caas-7DS, derived from Libellula, coincided with the slow rusting and slow mildewing locus Lr34/Yr18/Pm38. These results and the identified markers could be useful for wheat breeders aiming for durable resistance to both powdery mildew and stripe rust.

Download Full-text