scholarly journals Analysis of Metabolomic Changes in Lettuce Leaves under Low Nitrogen and Phosphorus Deficiencies Stresses

Agriculture ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 406 ◽  
Author(s):  
Hongyan Gao ◽  
Hanping Mao ◽  
Ikram Ullah

Nitrogen and phosphorus limitation affect the growth, development, and productivity of lettuce, which exert a marked influence on metabolites. To compare the influence of low-nitrogen and low-phosphorus stresses on various metabolites of lettuce leaves, experiments were performed under three conditions of treatment—low-nitrogen stress, low-phosphorus stress, and normal samples. Metabolomic analyses were conducted based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Principle components analysis yielded distinctive clustering information among the holistic samples; fold change analysis, t-test and orthogonal partial least square discriminant analysis were used for the selection of metabolic biomarkers. Ten pathways were selected which were significantly enriched by metabolic biomarkers. Metabolic biomarkers were screened by fold change (FC) value, p-value and variable importance in the projection (VIP) value, low-nitrogen and low-phosphorus stresses caused an increase in 16 metabolites (FC > 2, p-value < 0.05, VIP > 1) and a decrease in 26 metabolites (FC < 0.5, p-value < 0.05, VIP > 1). Outside of these, our results showed that inositol, p-hydroxybenzoic acid, stachyose, dinoseb, and 7, 8-dihydroxycoumarin increase in low-nitrogen stress samples. Low-phosphorus stress caused accumulation of citrate, isocitrate, l-5-oxoproline, succinate, and histamine, which may be considered potential metabolic biomarkers. The metabolites could be used to monitor the nitrogen and phosphorus status of lettuce and to guide appropriate fertilization regimens.

Author(s):  
Brigitta Tóth ◽  
Angeline van Biljon ◽  
Maryke Labuschagne

Iron (Fe) and zinc (Zn) deficiency in cereal grains has deleterious effects on the health of millions of people, especially in developing countries. As wheat, as a staple crop, is consumed in large quantities, its micronutrient content is important. Crops in Africa are often grown under low nitrogen (N) and low phosphorous (P) conditions. The aim of this study was to determine the effect of low N and low P stress on Fe and Zn and phytic acid concentration, in two commercial spring wheat cultivars with excellent baking quality. The two cultivars did not differ significantly for the measured characteristics. Across all treatments the average values for Fe varied between 19.60-28.61 mg kg-1, Zn between 17.68-33.79 mg kg-1 and phytic acid between 5.03-6.92 mg g-1. Low P stress lead to the highest values of Fe and Zn, and the lowest value for phytic acid. Phytic acid:Fe and phytic acid:Zn ratios were also highly significantly reduced under low P stress conditions. Low N conditions caused significantly increased Zn levels.&nbsp; Despite this, the phytic acid:Fe and phytic acid:Zn ratios were relatively high under all conditions, indicating a low bioavailability of both Fe and Zn in these wheat cultivars.


2006 ◽  
Vol 60 (5) ◽  
pp. 617-631 ◽  
Author(s):  
Xingming Lian ◽  
Shiping Wang ◽  
Jianwei Zhang ◽  
Qi Feng ◽  
Lida Zhang ◽  
...  

2019 ◽  
Vol 143 ◽  
pp. 1-10 ◽  
Author(s):  
Benjamin A. Babst ◽  
Fei Gao ◽  
Lucia M. Acosta-Gamboa ◽  
Abhijit Karve ◽  
Michael J. Schueller ◽  
...  

2020 ◽  
Vol 26 (9) ◽  
pp. 1897-1910
Author(s):  
Abdul Malik Solangi ◽  
Hira Khanzada ◽  
Ghulam Mustafa Wassan ◽  
Adnan Rasheed ◽  
Ayaz Ali Keerio ◽  
...  

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