Characterization of Interspecific Hybrids between Flowering Chinese Cabbage and Chinese Kale

Interspecific hybridization is considered to be an important driving force in the evolution, diversification, and formation of plant species. We selected one flowering Chinese cabbage variety and three Chinese kale varieties to make hybrids. Heterologous haploid offspring were obtained by embryo rescue and heterologous diploids were obtained by colchicine doubling. A total of 108 individuals of the F2, F3 and F4 generations from three parental combinations were investigated for field traits and SSR (simple sequence repeats) markers. The results showed trait separation and the appearance and disappearance of SSR bands in the hybrids, showing significant differences among parental combinations and among the different generations. This proved that the phenotypes of the initial generations of allopolyploids were not stable. This study not only enriches the genetic resources available for breeding flowering Chinese cabbage and Chinese kale, but lays a theoretical foundation for exploring the segregation of traits in distant hybrids and in different generations.

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Characterization of interspecific hybrids between flowering Chinese cabbage and broccoli

Scientia Horticulturae ◽

10.1016/j.scienta.2018.06.059 ◽

2018 ◽

Vol 240 ◽

pp. 552-557 ◽

Cited By ~ 1

Author(s):

Yunxiao Wei ◽

Mingzhao Zhu ◽

Haiyun Qiao ◽

Fei Li ◽

Shujiang Zhang ◽

...

Keyword(s):

Chinese Cabbage ◽

Interspecific Hybrids ◽

Flowering Chinese Cabbage

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DNA Fingerprinting and Characterization of some Egyptian Date Palm Cultivars Using Simple Sequence Repeats (SSRs)

Journal of Plant Production Sciences ◽

10.21608/jpps.2015.7402 ◽

2015 ◽

Vol 3 (1) ◽

pp. 31-41

Author(s):

Mohammed Hussein ◽

Samah Sabry ◽

Manal Eid

Keyword(s):

Dna Fingerprinting ◽

Simple Sequence Repeats ◽

Date Palm ◽

Simple Sequence

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Genetic Diversity and Characterization of a Core Collection of Malus Germplasm Using Simple Sequence Repeats (SSRs)

Plant Molecular Biology Reporter ◽

10.1007/s11105-011-0399-x ◽

2011 ◽

Vol 30 (4) ◽

pp. 827-837 ◽

Cited By ~ 35

Author(s):

Sarah M. Potts ◽

Yuepeng Han ◽

M. Awais Khan ◽

Mosbah M. Kushad ◽

A. Lane Rayburn ◽

...

Keyword(s):

Genetic Diversity ◽

Simple Sequence Repeats ◽

Core Collection ◽

Simple Sequence

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SIMPLE SEQUENCE REPEATS (SSRS) FOR DIVERSITY CHARACTERIZATION OF GUAVA (PSIDIUM GUAJAVA L.)

Acta Horticulturae ◽

10.17660/actahortic.2010.849.17 ◽

2010 ◽

pp. 155-162 ◽

Cited By ~ 3

Author(s):

J. Valdés-Infante ◽

N.N. Rodríguez ◽

B. Velásquez ◽

D. Rivero ◽

F. Martínez ◽

...

Keyword(s):

Simple Sequence Repeats ◽

Psidium Guajava ◽

Simple Sequence

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Characterization of Simple Sequence Repeats (SSRs) in Ciliated Protists Inferred by Comparative Genomics

Microorganisms ◽

10.3390/microorganisms8050662 ◽

2020 ◽

Vol 8 (5) ◽

pp. 662 ◽

Cited By ~ 1

Author(s):

Yuan Li ◽

Xiao Chen ◽

Kun Wu ◽

Jiao Pan ◽

Hongan Long ◽

...

Keyword(s):

Simple Sequence Repeats ◽

Evolutionary History ◽

Rapid Evolution ◽

Genome Diversity ◽

Unicellular Eukaryotes ◽

Genome Wide ◽

Species Analysis ◽

Simple Sequence ◽

High Diversity

Simple sequence repeats (SSRs) are prevalent in the genomes of all organisms. They are widely used as genetic markers, and are insertion/deletion mutation hotspots, which directly influence genome evolution. However, little is known about such important genomic components in ciliated protists, a large group of unicellular eukaryotes with extremely long evolutionary history and genome diversity. With recent publications of multiple ciliate genomes, we start to get a chance to explore perfect SSRs with motif size 1–100 bp and at least three motif repeats in nine species of two ciliate classes, Oligohymenophorea and Spirotrichea. We found that homopolymers are the most prevalent SSRs in these A/T-rich species, with AAA (lysine, charged amino acid; also seen as an SSR with one-adenine motif repeated three times) being the codons repeated at the highest frequencies in coding SSR regions, consistent with the widespread alveolin proteins rich in lysine repeats as found in Tetrahymena. Micronuclear SSRs are universally more abundant than the macronuclear ones of the same motif-size, except for the 8-bp-motif SSRs in extensively fragmented chromosomes. Both the abundance and A/T content of SSRs decrease as motif-size increases, while the abundance is positively correlated with the A/T content of the genome. Also, smaller genomes have lower proportions of coding SSRs out of all SSRs in Paramecium species. This genome-wide and cross-species analysis reveals the high diversity of SSRs and reflects the rapid evolution of these simple repetitive elements in ciliate genomes.

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