Shift in Circulating Serum Protein Fraction (SPF) Levels of Pregnant Jennies and Nutritional Related Aspects at Early-, Mid- and Late Gestation

A viable tool for the monitoring of the systemic condition of the pregnant jenny may be the determination of serum protein fraction (SPF) levels, including metabolic profiling. Tissue development and composition of the growing fetus requires the mother to provide adequate nutrients to its body parts and organs. In this regard, body fluid distribution and strategic molecule transportation can be screened using SPF electropherograms and analysis of intermediate metabolites. The nutritional and health status of 12 jennies (age: 5–8 years; BW at the start: 135–138 kg; Body Condition Score, BCS [1 to 5 points] = 2.25–2.50; 4th month of gestation) were monitored throughout gestation (approximate gestation period 350–356 d). All animals were pasture-fed and were offered hay ad libitum. Individual blood samples were collected within the 4th, 7th, and 10th month following conception (ultrasound scanning). Serum biochemistry, in particular, the analysis of 6 fractions of serum proteins was carried out. The significant decrease in circulating albumin in jennies from mid- to late-gestation (p < 0.001) suggests a considerable role of dietary amino acids in the synthesis of protein for fetal tissue formation as well as body fluid distribution and blood pressure control of the jenny in those stages. Moreover, α1-globulin decreased significantly in late gestation (p < 0.047), corresponding to major organ development in the terminal fetus and supported by lipid transportation in the bloodstream of the jenny. Similarly, α2-globulin decreased in late gestation (p < 0.054) as haptoglobin, an important component for the transport of free circulating hemoglobin, is likely used for fetal synthesis. Mid-gestation, appears to be a crucial moment for adequate dietary nutrient supplementation in order to prevent homeostasis perturbation of jennies, as observed in this trial.

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STUDIES ON EQUINE INFECTIOUS ANEMIA VIRUS IN TISSUE CULTURE : VII. INFLUENCE OF BOVINE SERUM ON EQUINE LEUCOCYTE CULTURE TO BE USED FOR GROWTH OF EQUINE INFECTIOUS ANEMIA VIRUS WITH SPECIAL REFERENCE TO RELATIONSHIP BETWEEN SERUM PROTEIN FRACTION PATTERN AND LEUCOCYTE CULTURE

The Japanese Journal of Veterinary Science ◽

10.1292/jvms1939.28.119 ◽

1966 ◽

Vol 28 (3) ◽

pp. 119-128_2

Author(s):

Sachio WATANABE

Keyword(s):

Tissue Culture ◽

Special Reference ◽

Serum Protein ◽

Bovine Serum ◽

Protein Fraction ◽

Equine Infectious Anemia Virus ◽

Serum Protein Fraction ◽

Leucocyte Culture ◽

Equine Infectious Anemia ◽

Anemia Virus

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Age and androgen-related changes in morphological parameters, haematological indices and serum protein fraction in common vole (Microtus arvalis Pall.) growing in different photoperiods

Comparative Biochemistry and Physiology Part A Physiology ◽

10.1016/0300-9629(84)90423-7 ◽

1984 ◽

Vol 79 (2) ◽

pp. 241-249 ◽

Cited By ~ 8

Author(s):

Anna Dobrowolska ◽

Joanna Gromadzka-Ostrowska

Keyword(s):

Serum Protein ◽

Protein Fraction ◽

Common Vole ◽

Morphological Parameters ◽

Microtus Arvalis ◽

Serum Protein Fraction ◽

Haematological Indices

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The Presence of M Protein Is Strongly Associated with Very Poor Prognosis in Patients with Extranodal Diffuse Large B-Cell Lymphoma

Blood ◽

10.1182/blood.v126.23.5638.5638 ◽

2015 ◽

Vol 126 (23) ◽

pp. 5638-5368 ◽

Cited By ~ 1

Author(s):

Hideaki Nitta ◽

Yasuhito Terui ◽

Masahiro Yokoyama ◽

Yuko Mishima ◽

Noriko Nishimura ◽

...

Keyword(s):

Risk Factors ◽

Prognostic Factors ◽

Serum Protein ◽

Protein Fraction ◽

Performance Status ◽

B Cell Lymphoma ◽

M Protein ◽

Ki 67 ◽

Serum Protein Fraction ◽

Cox Proportional Hazard

Abstract Introduction Several studies concerning extranodal diffuse large B-cell lymphoma (DLBCL) have been reported sporadically. However, no new, valuable prognostic factors have been reported since several risk factors, such as a high international prognostic index (IPI) score, elevated lactate dehydrogenase (LDH) level, poor Eastern Cooperative Oncology Group (ECOG) performance status (PS), advanced stage, and extranodal sites ≥2, were identified. Methods To identify new and valuable prognostic factors, we reviewed the medical records of patients with nodal and extranodal DLBCL who were newly diagnosed at the Cancer Institute Hospital of the Japanese Foundation for Cancer Research from February 2005 to September 2014, and retrospectively analyzed the data of a total of 463 consecutive DLBCL patients. The cases were nodal DLBCL in 237 patients and extranodal DLBCL in 226 patients. We investigated the relationships between overall survival (OS), progression-free survival (PFS), and age, gender, LDH level, beta-2 microglobulin level (b2MG), performance status (PS), stage, extranodal sites ≥2, Ki-67 index, and M protein in serum protein fraction electrophoresis at diagnosis. Univariate and multivariate analyses of estimated risk factors for OS and PFS in extranodal DLBCL patients were performed using the log-rank test and Cox proportional hazard regression analysis. Results In patients with extranodal DLBCL, the median age was 67 years (range, 20-89 years). The median follow-up was 41 months (range, 1-115 months). A serum electrophoresis study detected M protein in 7 patients (3.1%). To adjust the impact of age, gender, LDH level, b2MG, PS, stage, extranodal sites ≥2, Ki-67 index, and the presence of M protein in serum protein fraction electrophoresis at diagnosis for other significant factors for OS, we identified the following risk factors in extranodal DLBCL by univariate analysis: elevated LDH level, elevated b2MG level, stage ≥3, extranodal sites ≥2, and the presence of M protein. Then, we performed multivariate analyses by using all of these factors in the Cox proportional hazard model. M protein (HR 6.78, 95% CI 2.19-20.96, P<0.001) and extranodal sites ≥2 (HR 3.71, 95% CI 1.77-7.80, P<0.001) were identified as independent significant prognostic factors for OS in extranodal DLBCL. Furthermore, we also identified M protein (HR 3.81, 95% CI 1.25-11.60, P=0.019) and extranodal sites ≥2 (HR 2.98, 95% CI 1.45-6.14, P=0.003) as independent significant prognostic factors for PFS by multivariate analysis. Four out of seven patients with M protein died due to lymphoma progression. Median overall survival in patients with extranodal DLBCL with M protein was 12 months (range, 2-114 months) compared with 44 months without M protein (range, 1-115 months, P=0.0038). On the other hand, in patients with nodal DLBCL, M protein (n=10) was not significantly associated with poor OS and PFS. Conclusions These results suggest that the presence of M protein in serum protein fraction electrophoresis is significantly associated with very poor OS and PFS in patients with extranodal DLBCL, but not nodal DLBCL. Extranodal DLBCL with M protein is a rare and very poor prognostic subset of DLBCL. Figure 1. Figure 1. Disclosures Yokoyama: Chugai Pharmaceutical CO., LTD.: Consultancy. Mishima:Chugai Pharmaceutical CO., LTD.: Consultancy. Nishimura:Chugai Pharmaceutical CO., LTD.: Consultancy. Hatake:Chugai Pharmaceutical CO., LTD.: Other: lecture speaking.

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