scholarly journals Isolation, Identification and Characterization of a Novel Megalocytivirus from Cultured Tilapia (Oreochromis spp.) from Southern California, USA

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3524
Author(s):  
Khalid Shahin ◽  
Kuttichantran Subramaniam ◽  
Alvin C. Camus ◽  
Zeinab Yazdi ◽  
Susan Yun ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Southern California ◽  
Phylogenetic Analyses ◽  
Head Kidney ◽  
Cell Culture Supernatant ◽  
Post Inoculation ◽  
Polymerase Gene ◽  
European Chub ◽  
Dna Polymerase Gene ◽  
Pcr Targeting

In spring 2019, diseased four-month-old tilapia (Oreochromis spp.) from an aquaculture farm in Southern California, USA were received for diagnostic evaluation with signs of lethargy, anorexia, abnormal swimming, and low-level mortalities. At necropsy, non-specific external lesions were noted including fin erosion, cutaneous melanosis, gill pallor, and coelomic distension. Internal changes included ascites, hepatomegaly, renomegaly, splenomegaly, and multifocal yellow-white nodules in the spleen and kidney. Cultures of spleen and kidney produced bacterial colonies identified as Francisella orientalis. Homogenized samples of gill, brain, liver, spleen, and kidney inoculated onto Mozambique tilapia brain cells (OmB) developed cytopathic effects, characterized by rounding of cells and detaching from the monolayer 6–10 days post-inoculation at 25 °C. Transmission electron microscopy revealed 115.4 ± 5.8 nm icosahedral virions with dense central cores in the cytoplasm of OmB cells. A consensus PCR, targeting the DNA polymerase gene of large double-stranded DNA viruses, performed on cell culture supernatant yielded a sequence consistent with an iridovirus. Phylogenetic analyses based on the concatenated full length major capsid protein and DNA polymerase gene sequences supported the tilapia virus as a novel species within the genus Megalocytivirus, most closely related to scale drop disease virus and European chub iridovirus. An intracoelomic injection challenge in Nile tilapia (O. niloticus) fingerlings resulted in 39% mortality after 16 days. Histopathology revealed necrosis of head kidney and splenic hematopoietic tissues.

Nucleotide sequence and phylogenetic analyses of the DNA polymerase gene of Anticarsia gemmatalis nucleopolyhedrovirus

2005 ◽  
Vol 110 (1-2) ◽  
pp. 99-109 ◽  
Author(s):  
Caren Cristina Dalmolin ◽  
Felipe R. da Silva ◽  
Luciane V. Mello ◽  
Daniel J. Rigden ◽  
Maria Elita B. Castro
Keyword(s):  
Nucleotide Sequence ◽  
Dna Polymerase ◽  
Phylogenetic Analyses ◽  
Anticarsia Gemmatalis ◽  
Polymerase Gene ◽  
Dna Polymerase Gene

Partial sequence of the DNA-dependent DNA polymerase gene of fowl adenoviruses: A reference panel for a general diagnostic PCR in poultry

2011 ◽  
Vol 59 (2) ◽  
pp. 279-285 ◽  
Author(s):  
Győző Kaján ◽  
Soroush Sameti ◽  
Mária Benkő
Keyword(s):  
Dna Polymerase ◽  
Virus Species ◽  
Polymerase Gene ◽  
Species Classification ◽  
Diagnostic Pcr ◽  
Serological Typing ◽  
The Family ◽  
Efficient Alternative ◽  
Dna Polymerase Gene ◽  
Pcr Targeting

Adenoviruses are frequent infectious agents in different poultry species. The traditional, serological typing of new isolates by virus neutralisation tests is now in transition to be replaced by PCR and sequencing. The first PCRs, recommended for the detection of adenoviruses, had been designed to target the gene of the major capsid protein, the hexon. In birds, members of three different genera of the familyAdenoviridaemay occur. Accordingly, three specific hexon PCRs had to be elaborated for the detection of adenoviruses in poultry. A significantly more sensitive PCR, targeting the viral DNA-dependent DNA polymerase gene, has been described recently. This method proved to be an efficient alternative for the general detection of adenoviruses irrespective of their genus affiliation. Fowl adenoviruses (FAdVs), isolated from chicken to date, comprise twelve serotypes classified into five virus species (FAdV-A to E). The polymerase gene sequence has been determined yet only from three FAdV types representing three species. In the present work, the panel of polymerase gene sequences was completed with those of the rest of FAdVs. The newly determined sequences will facilitate the identification of new FAdV isolates as an existing species or as a putative new FAdV. Once the polymerase sequence is known, more specific PCRs for the amplification of the hexon and other genes can be designed and performed according to the preliminary species classification.

scholarly journals Detection and partial genetic characterisation of novel avi- and siadenoviruses in racing and fancy pigeons (Columba livia domestica)

2016 ◽  
Vol 64 (4) ◽  
pp. 514-528 ◽  
Author(s):  
Mónika Z. Ballmann ◽  
Balázs Harrach
Keyword(s):  
Sequence Analysis ◽  
Dna Sequence ◽  
Dna Polymerase ◽  
Nested Pcr ◽  
Columba Livia ◽  
Hexon Gene ◽  
Polymerase Gene ◽  
Viral Dna ◽  
Phylogeny Analysis ◽  
Pcr Targeting

Up to now, only a single adenovirus (AdV) isolate seemingly specific for pigeons, hence named pigeon AdV-1 (PiAdV-1), has been characterised at DNA sequence level. In the present work, the prevalence and diversity of AdVs occurring in domestic pigeon were examined by a survey performed on randomly collected samples using a very efficient, consensus nested PCR targeting the viral DNA polymerase gene. The newly detected viruses were characterised by sequencing and phylogeny analysis. Amplification of additional genome fragments was attempted by the use of several other PCR methods aiming at the hexon gene. During a 4-year survey, samples from dead or live, healthy pigeons originating from 27 lofts were examined in Hungary. Almost 50% of the samples (48 out of 97) proved to be positive for AdV. Sequence analysis revealed the presence of four hitherto unknown pigeon AdV types. PiAdV-1 was also identified in one sample. Two novel viruses named PiAdV-2 and -3 were found to belong to the genus Aviadenovirus, and two other novel types (PiAdV-4 and -5) to the genus Siadenovirus. This is the first report on the occurrence of siadenoviruses in birds belonging to the order Columbiformes. Approximately two-thirds of the PiAdV-2 genome was sequenced and analysed.

scholarly journals Characterization of intein homing endonuclease encoded in the DNA polymerase gene ofThermococcus marinus

2009 ◽  
Vol 297 (2) ◽  
pp. 180-188 ◽  
Author(s):  
Heejin Bae ◽  
Kee Pum Kim ◽  
Jung Min Song ◽  
Jun-Hwan Kim ◽  
Joo-Sung Yang ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Homing Endonuclease ◽  
Polymerase Gene ◽  
Dna Polymerase Gene
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