scholarly journals Characterization of Anti-Ana o 3 Monoclonal Antibodies and Their Application in Comparing Brazilian Cashew Cultivars

Antibodies ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 46
Author(s):  
Christopher P. Mattison ◽  
Barry Vant-Hull ◽  
Ana Cecilia Ribeiro de Castro ◽  
Heidi J. Chial ◽  
Yvette Bren-Mattison ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
In Silico ◽  
Reducing Agent ◽  
Cashew Nut ◽  
Conformational Epitopes ◽  
In Silico Modeling ◽  
Linear Epitopes ◽  
Allergen Content

Ana o 3 is an immuno-dominant cashew nut allergen. Four monoclonal antibodies to Ana o 3 (2H5, 6B9C1, 19C9A2, and 5B7F8) were characterized by ELISA and in silico modeling. The 2H5 antibody was the only antibody specific for cashew nut extract. In addition to cashew nut extract, the 6B9C1 and 19C9A2 antibodies recognized pistachio extract, and the 5B7F8 recognized pecan extract. All four antibodies recognized both recombinant Ana o 3.0101 and native Ana o 3. ELISA assays following treatment of purified Ana o 3 with a reducing agent indicated that the 6B9C1 and 19C9A2 antibodies likely recognize conformational epitopes, while the 2H5 and 5B7F8 antibodies likely recognize linear epitopes. In silico modeling predicted distinct epitopes for each of the anti-Ana o 3 antibodies. Screening extracts from 11 Brazilian cashew nut cultivars using all four antibodies showed slight differences in Ana o 3 bindings, demonstrating that these antibodies could identify cultivars with varying allergen content.

scholarly journals Comparison and in-silico modeling of monoclonal antibodies recognizing the immuno-dominant Ana o 3 cashew nut allergen

2020 ◽  
Vol 13 (8) ◽  
pp. 100314
Author(s):  
Christopher Mattison ◽  
Barry Vant-Hull ◽  
Yvette Bren-Mattison ◽  
Casey Grimm
Keyword(s):  
Monoclonal Antibodies ◽  
In Silico ◽  
Cashew Nut ◽  
In Silico Modeling

scholarly journals Production and characterization of polyclonal and monoclonal antibodies against human thromboxane synthase

Blood ◽  
1990 ◽  
Vol 76 (1) ◽  
pp. 80-85 ◽  
Author(s):  
R Nusing ◽  
MP Wernet ◽  
V Ullrich
Keyword(s):  
Enzyme Activity ◽  
Monoclonal Antibodies ◽  
Western Blot ◽  
Human Lung ◽  
Human Platelet ◽  
Human Lung Tissue ◽  
Western Blots ◽  
Conformational Epitopes ◽  
Platelet Thromboxane

Abstract Polyclonal and monoclonal antibodies (MoAbs) were raised against human platelet thromboxane (Tx) synthase. Neither the antiserum nor the MoAbs inhibited the enzyme activity significantly. Three MoAbs, Tu 300, Kon 6, and Kon 7, were purified and further characterized. They are monospecific as shown by activity precipitation or Western blot analysis, and recognized different epitopes on Tx-synthase. Tu 300 could precipitate the enzyme and recognized conformational epitopes, whereas Kon 6 and Kon 7 only reacted in Western blots. Antibody Tu 300 can be used in immunohistology but shows no crossreactivity with Tx- synthase from other species. In human lung tissue staining with peroxidase, coupled Tu 300 was only found in alveolar macrophages.

scholarly journals Production and characterization of monoclonal antibodies anti fragment Fc of bovine IgG

2010 ◽  
Vol 53 (1) ◽  
pp. 105-114 ◽  
Author(s):  
Tânia Regina Penha ◽  
Ernesto Renato Krüger ◽  
Vanete Thomaz-Soccol ◽  
Jorge Victor Bacila Agottani ◽  
Flávio Hiroshi Itano ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Western Blot ◽  
Immunoglobulin G ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Reducing Agent ◽  
Polyacrylamide Gel ◽  
Bovine Immunoglobulin

The aim of this work was to produce and characterize monoclonal antibodies anti bovine immunoglobulin G (IgG). Out of seven hybridomas, two were chosen based on the ELISA'S absorbance values and were labeled B4F11 and B3H12. These monoclonals were analyzed through Western Blot for IgG fragments obtained by proteolysis with papain, separated by electrophoresis in polyacrylamide gel electrophoresis with β-mercaptoetanol as reducing agent. This revealed that, possibly, the B4F11 was directed to a conformational antigen, and that B3H12 reacted in a specific fashion with Fc (Bovine IgG crystallizable fragment). This antibody could be used in the development of reagents to immunoassays relevant for research and diagnosis.

scholarly journals Monoclonal Antibodies against the Adeno-Associated Virus Type 2 (AAV-2) Capsid: Epitope Mapping and Identification of Capsid Domains Involved in AAV-2–Cell Interaction and Neutralization of AAV-2 Infection

2000 ◽  
Vol 74 (19) ◽  
pp. 9281-9293 ◽  
Author(s):  
Christiane E. Wobus ◽  
Barbara Hügle-Dörr ◽  
Anne Girod ◽  
Gabriele Petersen ◽  
Michael Hallek ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Virus Type ◽  
Epitope Mapping ◽  
Cell Interaction ◽  
Phage Display Library ◽  
Adeno Associated Virus ◽  
Loop Region ◽  
Conformational Epitopes ◽  
Linear Epitopes

ABSTRACT The previously characterized monoclonal antibodies (MAbs) A1, A69, B1, and A20 are directed against assembled or nonassembled adeno-associated virus type 2 (AAV-2) capsid proteins (A. Wistuba, A. Kern, S. Weger, D. Grimm, and J. A. Kleinschmidt, J. Virol. 71:1341–1352, 1997). Here we describe the linear epitopes of A1, A69, and B1 which reside in VP1, VP2, and VP3, respectively, using gene fragment phage display library, peptide scan, and peptide competition experiments. In addition, MAbs A20, C24-B, C37-B, and D3 directed against conformational epitopes on AAV-2 capsids were characterized. Epitope sequences on the capsid surface were identified by enzyme-linked immunoabsorbent assay using AAV-2 mutants and AAV serotypes, peptide scan, and peptide competition experiments. A20 neutralizes infection following receptor attachment by binding an epitope formed during AAV-2 capsid assembly. The newly isolated antibodies C24-B and C37-B inhibit AAV-2 binding to cells, probably by recognizing a loop region involved in binding of AAV-2 to the cellular receptor. In contrast, binding of D3 to a loop near the predicted threefold spike does not neutralize AAV-2 infection. The identified antigenic regions on the AAV-2 capsid surface are discussed with respect to their possible roles in different steps of the viral life cycle.

Characterization of eleven antigenic groups in Trichinella genus and identification of stage and species markers

Parasitology ◽  
1997 ◽  
Vol 115 (6) ◽  
pp. 641-651 ◽  
Author(s):  
P. BOIREAU ◽  
M. VAYSSIER ◽  
J. F. FABIEN ◽  
C. PERRET ◽  
M. CALAMEL ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cross Sections ◽  
Trichinella Spiralis ◽  
Elisa Test ◽  
Soluble Antigen ◽  
Dot Blot ◽  
Infective Larvae ◽  
Linear Epitopes ◽  
Species Markers

Several monoclonal antibodies (Mabs) were raised against the L1 muscle stage (L1M) of Trichinella spiralis (Ts) and Trichinella pseudospiralis (Tp). Western blot analysis of various antigenic preparations established that Mabs described by different authors recognized 8 antigenic fractions (TSL1–TSL8) in crude extracts of infective larvae. The TSL1 fraction was immunodominant and present on the cuticle of different parasite stages. Mabs against Trichinella T5 (T5) and Ts were selected in order to extend the previous studies to another Trichinella phenotype. Only 35% of the selected Mabs recognized linear epitopes and 71% reacted with soluble or excretory–secretory antigens in a dot blot procedure and ELISA test. The targets of the Mabs were identified by immunoprecipitation with [35S]methionine-labelled L1M worm lysate. Mabs prepared from mice immunized with the whole parasite (T5) recognized a wider panel of antigens in different parasitic organs. Seven antigenic structures were distinguished on the cuticle and several epitopes were identified in the gut, haemolymph and stichocytes. Eleven antigenic groups were established according to their indirect immunofluorescence pattern on cross-sections of the worm. Monoclonal antibodies raised against Ts soluble antigen mainly recognized epitopes in stichocytes and on the cuticle surface. All the selected Mabs recognized T5 and Trichinella britovi (Tb) strengthening the link between these 2 species. Four Mabs were used to differentiate antigenic structures among 6 Trichinella phenotypes and to develop a new tool to follow gene flow within the Trichinella genus.

scholarly journals Characterization of recombinant yellow fever-dengue vaccine viruses with human monoclonal antibodies targeting key conformational epitopes

Vaccine ◽  
2019 ◽  
Vol 37 (32) ◽  
pp. 4601-4609 ◽  
Author(s):  
Valerie Lecouturier ◽  
Catherine Berry ◽  
Aure Saulnier ◽  
Sophie Naville ◽  
Catherine Manin ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Yellow Fever ◽  
Dengue Vaccine ◽  
Human Monoclonal Antibodies ◽  
Conformational Epitopes

Synthesis and characterization of chitosan ascorbate nanoparticles for therapeutic inhibition for cervical cancer and their in silico modeling

2018 ◽  
Vol 62 ◽  
pp. 239-249 ◽  
Author(s):  
Vigneshkumar Sekar ◽  
Karthikeyan Rajendran ◽  
Sugumari Vallinayagam ◽  
Venkataraman Deepak ◽  
Surianarayanan Mahadevan
Keyword(s):  
Cervical Cancer ◽  
In Silico ◽  
Synthesis And Characterization ◽  
In Silico Modeling
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