scholarly journals Advanced Label-Free Laser Scanning Microscopy and Its Biological Imaging Application

2021 ◽  
Vol 11 (3) ◽  
pp. 1002
Author(s):  
Xue Wang ◽  
Xinchao Lu ◽  
Chengjun Huang
Keyword(s):  
Laser Scanning ◽  
Second Harmonic ◽  
Biological Tissues ◽  
Biological Imaging ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Coherent Raman ◽  
Surface Plasmon Microscopy

By eliminating the photodamage and photobleaching induced by high intensity laser and fluorescent molecular, the label-free laser scanning microscopy shows powerful capability for imaging and dynamic tracing to biological tissues and cells. In this review, three types of label-free laser scanning microscopies: laser scanning coherent Raman scattering microscopy, second harmonic generation microscopy and scanning localized surface plasmon microscopy are discussed with their fundamentals, features and recent progress. The applications of label-free biological imaging of these laser scanning microscopies are also introduced. Finally, the performance of the microscopies is compared and the limitation and perspectives are summarized.

scholarly journals Label‐free laser scanning microscopy targeting sentinel lymph node diagnostics: A feasibility study ex vivo

2020 ◽  
Vol 2 (3) ◽  
Author(s):  
Despoina Kantere ◽  
Jan Siarov ◽  
Shahin De Lara ◽  
Samad Parhizkar ◽  
Roger Olofsson Bagge ◽  
...  
Keyword(s):  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Feasibility Study ◽  
Laser Scanning ◽  
Ex Vivo ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Label Free

Second Harmonic Imaging Microscopy: A New Non-Linear Optical Modality for Cell Membrane Physiology

2000 ◽  
Vol 6 (S2) ◽  
pp. 810-811
Author(s):  
Paul Campagnola ◽  
Aaron Lewis ◽  
Leslie M. Loew
Keyword(s):  
Confocal Microscopy ◽  
Laser Scanning ◽  
Second Harmonic ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Photon Absorption ◽  
Photon Flux ◽  
Two Photon ◽  
Non Linear ◽  
Linear Optical

Confocal microscopy is an excellent high resolution method to image fluorescently labeled cells. However, the use of confocal microscopy to monitor physiological events, such as membrane potential changes, in living cells is hampered by photobleaching and phototoxicity. To reduce the collateral damage from excitation of fluorescent probes outside the optical slice, Webb and co-workers introduced the use of two-photon excited (TPE) fluorescence in laser scanning microscopy.1 Two-photon absorption depends on the square of the incident light intensity; this has the effect of confining excitation to the plane of focus where the photon flux density is greatest. The wavelength of the exciting light is in the near infrared facilitating penetration of thick tissues. Due to these significant advantages this methodology is rapidly gaining popularity as a tool for live cell and tissue imaging.To further exploit non-linear optical processes in laser scanning microscopy, we have developed surface second harmonic generation (SHG) as a powerful new imaging modality.

scholarly journals Multimodal nonlinear microscopy: A powerful label-free method for supporting standard diagnostics on biological tissues

2014 ◽  
Vol 07 (05) ◽  
pp. 1330008 ◽  
Author(s):  
Riccardo Cicchi ◽  
Francesco Saverio Pavone
Keyword(s):  
Laser Scanning ◽  
Imaging Techniques ◽  
Biological Tissues ◽  
Laser Scanning Microscopy ◽  
Tissue Imaging ◽  
Nonlinear Microscopy ◽  
Label Free ◽  
Free Alternative ◽  
Functional Scheme ◽  
Nonlinear Imaging

The large use of nonlinear laser scanning microscopy in the past decade paved the way for potential clinical application of this imaging technique. Modern nonlinear microscopy techniques offer promising label-free solutions to improve diagnostic performances on tissues. In particular, the combination of multiple nonlinear imaging techniques in the same microscope allows integrating morphological with functional information in a morpho-functional scheme. Such approach provides a high-resolution label-free alternative to both histological and immunohistochemical examination of tissues and is becoming increasingly popular among the clinical community. Nevertheless, several technical improvements, including automatic scanning and image analysis, are required before the technique represents a standard diagnostic method. In this review paper, we highlight the capabilities of multimodal nonlinear microscopy for tissue imaging, by providing various examples on colon, arterial and skin tissues. The comparison between images acquired using multimodal nonlinear microscopy and histology shows a good agreement between the two methods. The results demonstrate that multimodal nonlinear microscopy is a powerful label-free alternative to standard histopathological methods and has the potential to find a stable place in the clinical setting in the near future.

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