Hsp60 Quantification in Human Gastric Mucosa Shows Differences between Pathologies with Various Degrees of Proliferation and Malignancy Grade

Background: Stomach diseases are an important sector of gastroenterology, including proliferative benign; premalignant; and malignant pathologies of the gastric mucosa, such as gastritis, hyperplastic polyps, metaplasia, dysplasia, and adenocarcinoma. There are data showing quantitative changes in chaperone system (CS) components in inflammatory pathologies and tumorigenesis, but their roles are poorly understood, and information pertaining to the stomach is scarce. Here, we report our findings on one CS component, the chaperone Hsp60, which we studied first considering its essential functions inside and outside mitochondria. Methods: We performed immunohistochemical experiments for Hsp60 in different samples of gastric mucosa. Results: The data obtained by quantitative analysis showed that the average percentages of Hsp60 were of 32.8 in normal mucosa; 33.5 in mild-to-moderate gastritis; 51.8 in severe gastritis; 58.5 in hyperplastic polyps; 67.0 in intestinal metaplasia; 89.4 in gastric dysplasia; and 92.5 in adenocarcinomas. Noteworthy were: (i) the difference between dysplasia and adenocarcinoma with the other pathologies; (ii) the progressive increase in Hsp60 from gastritis to hyperplastic polyp, gastric dysplasia, and gastric carcinoma; and (iii) the correlation of Hsp60 levels with histological patterns of cell proliferation and, especially, with tissue malignancy grades. Conclusions: This trend likely reflects the mounting need for cells for Hsp60 as they progress toward malignancy and is a useful indicator in differential diagnosis, as well as the call for research on the mechanisms underpinning the increase in Hsp60 and its possible roles in carcinogenesis.

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Expression of Surfactant Protein D in the Human Gastric Mucosa and during Helicobacter pylori Infection

Infection and Immunity ◽

10.1128/iai.70.3.1481-1487.2002 ◽

2002 ◽

Vol 70 (3) ◽

pp. 1481-1487 ◽

Cited By ~ 41

Author(s):

Emma Murray ◽

Wafa Khamri ◽

Marjorie M. Walker ◽

Paul Eggleton ◽

Anthony P. Moran ◽

...

Keyword(s):

Gastric Mucosa ◽

Surfactant Protein ◽

Normal Mucosa ◽

Innate Immune ◽

Surfactant Protein D ◽

Human Gastric Mucosa ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Immune Molecule ◽

H Pylori

ABSTRACT Helicobacter pylori establishes persistent infection of gastric mucosa with diverse clinical outcomes. The innate immune molecule surfactant protein D (SP-D) binds selectively to microorganisms, inducing aggregation and phagocytosis. In this study, we demonstrated the expression of SP-D in gastric mucosa by reverse transcription-PCR and immuohistochemical analysis. SP-D is present at the luminal surface and within the gastric pits, with maximal expression at the surface. Levels of expression are significantly increased in H. pylori-associated gastritis compared to those in the normal mucosa. Immunofluorescence microscopy was used to demonstrate binding and agglutination of H. pylori by SP-D in a lectin-specific manner. These activities resulted in a 50% reduction in the motility of H. pylori, as judged on the basis of curvilinear velocity measured by using a Hobson BacTracker. Lipopolysaccharides extracted from three H. pylori strains were shown to bind SP-D in a concentration-dependent manner, and there was marked variation in the avidity of binding among the strains. SP-D may therefore play a significant role in the innate immune response to H. pylori infection.

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H. pylori down-regulates TRAIL (DR4)-receptors in the human gastric mucosa, a possible mechanism for chronic persistence

Gastroenterology ◽

10.1016/s0016-5085(01)80401-7 ◽

2001 ◽

Vol 120 (5) ◽

pp. A81-A81

Author(s):

B NEU ◽

R RAD ◽

M NEUHOFER ◽

C TRAUTWEIN ◽

M GERHARD ◽

...

Keyword(s):

Gastric Mucosa ◽

Human Gastric Mucosa ◽

H Pylori

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The suppresive effect of a fermented milk containing lactobacilli on Helicobacter pylori in human gastric mucosa

Gastroenterology ◽

10.1016/s0016-5085(01)82935-8 ◽

2001 ◽

Vol 120 (5) ◽

pp. A590-A590 ◽

Cited By ~ 1

Author(s):

M PARK ◽

J KIM ◽

H JUNG ◽

I SONG ◽

J LEE

Keyword(s):

Helicobacter Pylori ◽

Gastric Mucosa ◽

Fermented Milk ◽

Human Gastric Mucosa

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The Fine Structure of Normal Human Gastric Mucosa

Gastroenterology ◽

10.1016/s0016-5085(19)34740-7 ◽

1964 ◽

Vol 47 (3) ◽

pp. 269-290 ◽

Cited By ~ 61

Author(s):

Clinton B. Lillibridge

Keyword(s):

Fine Structure ◽

Gastric Mucosa ◽

Human Gastric Mucosa ◽

Normal Human

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CURCUMIN UPREGULATES INDOLEAMINE 2,3-DIOXYGENASE (IDO) AND DOWNREGULATES INTERLEUKIN (IL)-17 PRODUCTION IN HELICOBACTER PYLORI-INFECTED HUMAN GASTRIC MUCOSA

10.26226/morressier.57c53841d462b80296c9ca88 ◽

2016 ◽

Author(s):

Tiziana Larussa

Keyword(s):

Helicobacter Pylori ◽

Gastric Mucosa ◽

Human Gastric Mucosa ◽

Indoleamine 2,3 Dioxygenase

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Purification and Properties of Acid Deoxyribonucleases of Human Gastric Mucosa and Cervix Uteri

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)42557-x ◽

1974 ◽

Vol 249 (12) ◽

pp. 3884-3889 ◽

Cited By ~ 1

Author(s):

Masayoshi Yamanaka ◽

Yoriaki Tsubota ◽

Motoaki Anai ◽

Koji Ishimatsu ◽

Makoto Okumura ◽

...

Keyword(s):

Gastric Mucosa ◽

Cervix Uteri ◽

Human Gastric Mucosa ◽

Purification And Properties

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Effect of Leukoreduction on Hematobiochemical Parameters and Storage Hemolysis in Canine Whole Blood Units

Animals ◽

10.3390/ani11040925 ◽

2021 ◽

Vol 11 (4) ◽

pp. 925

Author(s):

Maria Teresa Antognoni ◽

Maria Luisa Marenzoni ◽

Ambra Lisa Misia ◽

Luca Avellini ◽

Elisabetta Chiaradia ◽

...

Keyword(s):

Whole Blood ◽

Complete Blood Count ◽

Membrane Resistance ◽

Progressive Increase ◽

Rbc Membrane ◽

Morphological Index ◽

The Difference ◽

Hematobiochemical Parameters ◽

And Storage ◽

Individual Donor

Storage lesions (SLs) occur when the red blood cell quality is altered during the preservation of blood units. Pre-storage leukoreduction would limit the number of SLs. The aims of this study were to evaluate the effectiveness of a leukoreduction filter for human use and the effect of pre-storage leukoreduction on some ematobiochemical parameters in stored canine whole blood. Seven canine blood units were tested. Each one was divided into two units—one leukoreduced (LRWB) and one non-leukoreduced (nLRWB). On each unit, we determined the complete blood count (CBC), lactate-dehydrogenase (LDH), electrolytes (Na+, K+, Cl−), morphological index (MI) and hemolysis, on storage days 0, 7, 14, 21, 28, 35, and 42. Leukoreduction allowed a 98.30% recovery of the RBC count, retaining 99.69% and 94.91% of WBCs and PLTs, respectively. We detected a significant increase of LDH and MI with strongly higher values in nLRWB compared to LRWB. A progressive increase in electrolytes and LDH concentrations was observed as indices of stored hemolysis. LDH showed significantly lower values in LRWB units compared to nLRWB, suggesting its release from leukocytes. In the majority of units, hemolysis reached 1% on the 42nd day of storage. We assert the human leukoreduction filter effectiveness on canine whole blood, and we recommend using nLRWB before day 14, especially for critically ill patients. The difference of the basal hemolysis (day 0) percentages observed between subjects suggests that more studies should be performed to confirm a possible inter-individual donor biological variability of RBC membrane resistance, as happens in humans.

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Relation of Helicobacter pylori to the human gastric mucosa in chronic gastritis of the antrum.

Gut ◽

10.1136/gut.31.11.1230 ◽

1990 ◽

Vol 31 (11) ◽

pp. 1230-1236 ◽

Cited By ~ 61

Author(s):

L L Thomsen ◽

J B Gavin ◽

C Tasman-Jones

Keyword(s):

Helicobacter Pylori ◽

Gastric Mucosa ◽

Chronic Gastritis ◽

Human Gastric Mucosa

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Succinic and Malic Oxidase in Gastric Hydrochloric Acid Production

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1956.187.3.427 ◽

1956 ◽

Vol 187 (3) ◽

pp. 427-431 ◽

Cited By ~ 5

Author(s):

Joseph J. Vitale ◽

Oscar M. Jankelson ◽

Patricia Connors ◽

D. Mark Hegsted ◽

Norman Zamcheck

Keyword(s):

Gastric Mucosa ◽

Guinea Pig ◽

Succinic Dehydrogenase ◽

Titratable Acidity ◽

Spectrophotometric Analysis ◽

Parietal Cells ◽

Human Gastric Mucosa ◽

City Hospital ◽

Malic Dehydrogenase ◽

Oxidase System

Effect of histamine on the activity of succinic oxidase and malic dehydrogenase was studied in guinea pig and human gastric mucosa. Human tissue was obtained through the surgical services of the Boston City Hospital. Control value for the succinic oxidase system of the proximal half of the guinea pig stomach was approximately 480 ( Qo2 (N) (µl O2/mg nitrogen/hr.)). After histamine, this value rose to 550 in 30 minutes with a simultaneous rise in titratable acidity of the stomach contents. Animals fasted for 72 hours had a Qo2 (N) of approximately 500 and after histamine a Qo2 (N) of 700 was observed. Spectrophotometric analysis of succinic dehydrogenase and cytochrome oxidase activities, two of the major components of the succinic oxidase system, revealed that both components are increased following histamine administration. Malic dehydrogenase, however, was not affected by histamine treatment. Succinic dehydrogenase was demonstrated by histochemical localization and was concentrated below the superficial mucous layer where parietal cells were abundant. Succinic oxidase activity of human gastric mucosa was demonstrable only in those specimens containing abundant parietal cells. This study confirms the view that HCl production by parietal cells is associated with aerobic metabolism and is perhaps under enzymatic control. The study suggests that the succinic oxidase system may be involved in the production or secretion of HCl.

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