scholarly journals Late-Stage Functionalisation of Polycyclic (N-Hetero-) Aromatic Hydrocarbons by Detoxifying CYP5035S7 Monooxygenase of the White-Rot Fungus Polyporus arcularius

Biomolecules ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1708
Author(s):  
Nico D. Fessner ◽  
Christopher Grimm ◽  
Wolfgang Kroutil ◽  
Anton Glieder
Keyword(s):  
Aromatic Hydrocarbons ◽  
Active Site ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Cytochrome P450 Enzymes ◽  
Factors Influencing ◽  
Steric Factors ◽  
Polycyclic Aromatic ◽  
Insight Into

Functionalisation of polycyclic aromatic hydrocarbons (PAHs) and their N-heteroarene analogues (NPAHs) is a tedious synthetic endeavour that requires diverse bottom-up approaches. Cytochrome P450 enzymes of white-rot fungi were shown to participate in the fungal detoxification of xenobiotics and environmental hazards via hydroxylation of PAH compounds. In this paper, the recently discovered activity of the monooxygenase CYP5035S7 towards (N)PAHs was investigated in detail, and products formed from the substrates azulene, acenaphthene, fluorene, anthracene, and phenanthrene by whole-cell biocatalysis were isolated and characterised. The observed regioselectivity of CYP5035S7 could be explained by a combination of the substrate’s electron density and steric factors influencing the substrate orientation giving insight into the active-site geometry of the enzyme.

KEMAMPUAN DAN KEKUATAN BIOREMEDIASI AGEN HAYATI JAMUR FUNGI PELAPUK PUTIH

2018 ◽  
Vol 4 (3) ◽  
Author(s):  
Donawati Dkk
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Environmental Pollutants ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Huge Number ◽  
Tropical Country ◽  
The World ◽  
Polycyclic Aromatic

Indonesia is a tropical country and the richest country in the world for biodiversity, including fl ora and fauna, invertebrates, phytoplankton, bacteria, and huge number of fungus such as mushrooms, molds, mildews and smuts. White rot fungi is one of the ubiquitous creatures in Indonesia. Results indicated that white rot fungus has ability to degrade successfully some organic pollutansin the environment, such as polycyclic aromatic hydrocarbons (PAH), Polychlorinated Biphenyls (PCB’s), Trinitrotoluence (TNT), and DDT. This organism has ability to release intra and extra-cellular enzymes. There are three important rules in these degradation processes, called lignin modifi ed enzymes (LME’s) including Lignin peroxidase, Mn-dependent peroxidase and Laccase. White rot fungi contains all three enzymes and able to breakdown and mineralize several environmental pollutants into non toxic forms. This paper discuss several processes responsible for this degradation.Key words: White rot fungi, bioremediation, lignin modifi ed enzymes, persistent organic pollutants.

scholarly journals Polycyclic aromatic hydrocarbons (PAHs) degradation by laccase from a tropical white rot fungus Ganoderma lucidum

2009 ◽  
Vol 8 (21) ◽  
pp. 5897-5900 ◽  
Author(s):  
Punnapayak Hunsa ◽  
Prasongsuk Sehanat ◽  
Messner Kurt ◽  
Danmek Khanchai ◽  
Lotrakul Pongtharin
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Ganoderma Lucidum ◽  
White Rot ◽  
White Rot Fungus ◽  
Polycyclic Aromatic
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