scholarly journals Species and Fatty Acid Diversity of Desmodesmus (Chlorophyta) in a Local Japanese Area and Identification of New Docosahexaenoic Acid-Producing Species

Biomass ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 105-118
Author(s):  
Mikihide Demura ◽  
Seiji Noma ◽  
Nobuyuki Hayashi

Desmodesmus is a green microalgal genus that is frequently found in aquatic environments. Its high biomass productivity and potential as a source of lipids have attracted considerable attention. Although Desmodesmus is ubiquitous, it is difficult to identify; even within a small area, the diversity of the species and the fatty acids they produce are unknown. In this study, we performed scanning electron microscopy (SEM) and analyzed the genetic diversity of the internal transcribed spacer (ITS) region to accurately identify Desmodesmus in a local area of Japan (Saga City, Saga Pref.) and to assess its existence as a biological resource. In addition, we analyzed the fatty acid composition and content of the newly established strains. In total, 10 new strains were established, and 9 previously described species were identified. The presence of a cosmopolitan species indicated the global distribution of Desmodesmus. However, only regional species were found. One strain, dSgDes-b, did not form a clear clade with any described species in the phylogenetic analysis and had a characteristic ITS2 secondary structure. The cell wall of this strain exhibited a distinctive microstructure, and it produced docosahexaenoic acid (DHA); hence, the strain was described as a new species, Desmodesmus dohacommunis Demura sp. nov. Thus, useful information regarding the use of Desmodesmus as a bioresource was provided.

Phytotaxa ◽  
2019 ◽  
Vol 416 (4) ◽  
pp. 294-300
Author(s):  
SOUMITRA PALOI ◽  
ANNEMIEKE VERBEKEN ◽  
KRISHNENDU ACHARYA

Lactarius brunneocinnamomeus found in Shorea robusta (‎Dipterocarpaceae) dominated forest, is proposed as a new species. The species belongs to L. subg. Russularia, sect. Russularia. It is easily recognized by a light brown to orange brown pileus with concentric zones, milky white latex and a palisade to trichopalisade as a pileipellis. Its phylogenetic position inside the genus is based on the nuclear internal transcribed spacer (ITS) region. A complete description, drawings and photographs of the new species are provided. The new species is compared with morphologically similar and phylogenetically allied species.


Phytotaxa ◽  
2020 ◽  
Vol 430 (1) ◽  
pp. 17-24
Author(s):  
SHAN-SHAN CHU ◽  
DE-QUN WANG ◽  
HUA-SHENG PENG ◽  
LU-QI HUANG

Peucedanum huangshanense, a new species discovered in Anhui, China, is illustrated and described. Detailed morphological comparisons have showed that it is similar to P. praeruptorum but differs from the latter by having larger compound umbels (5–14 cm across), rays up to 25, and long-ovoid mericarps with lateral ribs narrowly winged. To explore the phylogenetic position of this species, nuclear ribosomal DNA internal transcribed spacer (ITS) region was sequenced for P. huangshanense and P. praeruptorum collected in different places. The morphological and molecular evidences support the hypothesis that P. huangshanense is a new distinct species.


Phytotaxa ◽  
2020 ◽  
Vol 475 (1) ◽  
pp. 43-51
Author(s):  
SHENG-HUA WU ◽  
CHIA-LING WEI ◽  
CHIUNG-CHIH CHANG

Sanghuangporus vitexicola (Hymenochaetales) is described as a new species based on collections made from Pingtung County, tropical South Taiwan. All studied basidiocarps grew on living trunks of Vitex negundo. This new species is characterized by having perennial, pileate basidiocarps; pore surface yellowish brown, pores 6–8 per mm; context 0.7–1.5 cm thick; setae ventricose to subulate, dark brown, 17–30 × 5–8 μm; basidiospores broadly ellipsoid, brownish, smooth, 4.2–4.8 × 3.2–3.7 μm, with 0.2–0.5 μm thick walls. Maximum likelihood and Bayesian inference phylogenies inferred from the internal transcribed spacer (ITS) region of rDNA indicated that six strains of Sanghuangporus viexicola formed a monophyletic group which is sister to S. zonatus. An identification key to known species of Sanghuangporus is provided.


Phytotaxa ◽  
2020 ◽  
Vol 452 (4) ◽  
pp. 268-277
Author(s):  
MUHAMMAD ALI ◽  
JUNAID KHAN ◽  
HIRA BASHIR ◽  
ABDUL REHMAN NIAZI ◽  
HASSAN SHER ◽  
...  

Infundibulicybe macrospora, a new species, is described and illustrated from the Himalayan moist temperate forests of Pakistan. The new species can be differentiated from close allies by its reddish orange to light orange pileus, whitish lamellae and stipe, and larger and broader basidiospores. The new species is also supported by molecular phylogenetic analysis inferred from internal transcribed spacer (ITS) region.


Phytotaxa ◽  
2015 ◽  
Vol 202 (2) ◽  
pp. 94 ◽  
Author(s):  
Yang Kun Li ◽  
Xin Zhang ◽  
Ye Yuan ◽  
Zheng Cao ◽  
Jun Feng Liang

Russula subrutilans sp. nov., a new species of Russula is described from southern China. It is unique for having buff pink to light congo-pink pileus, distant ventricose to subventricose lamellae with rare lamellulae, globose to broadly ellipsoid spores with bluntly conical warts forming a partial reticulum, and narrowly clavate to clavate cheilocystidia and pleurocysitidia with variable tips. Phylogenetic relationships among the new species and other closely related species in the genus are inferred based on the internal transcribed spacer (ITS) region.


Phytotaxa ◽  
2017 ◽  
Vol 313 (1) ◽  
pp. 130 ◽  
Author(s):  
LONG-FEI FU ◽  
SHI-LIAN HUANG ◽  
ALEX K. MONRO ◽  
YING LIU ◽  
FANG WEN ◽  
...  

Pilea nonggangensis Y. G. Wei, L. F. Fu & A. K. Monro, a new species from Guangxi, China is described and illustrated. The new species is morphologically most similar to P. basicordata from which it differs by being dioecious, having peltate leaf blades, membranous stipules, paired inflorescences and larger bracts. Nuclear ribosomal internal transcribed spacer (ITS) region and plastid trnL-F intron spacer (trnL-F) DNA sequence data from the new species and 20 local congeneric species are used to resolve the systematic position of the new species within Pilea. Despite vastly different morphology the molecular evidence suggests that P. nonggangensis is most closely related to P. pseudonotata. A Global Species Conservation Assessment classifies P. nonggangensis as Vulnerable (VU).


Karstenia ◽  
2021 ◽  
pp. 46-54
Author(s):  
Ertugrul Sesli

<em>Cortinarius gueneri</em> (subgen. <em>Telamonia</em> sect. <em>Laeti</em>) is described as a new species from Turkey based on morphological and molecular data. It can be recognized by the somewhat irregular conical to campanulate, translucent-striate, rusty brown pileus; pale yellowish to cinnamon brown broadly attached lamellae; pale salmon to pinkish brown stipe; narrowly amygdaloid to ellipsoid basidiospores; and its putative association with <em>Carpinus orientalis</em>. Full description of the new species is given with field photos, microscopic illustrations, and a short discussion. Phylogenetic analysis of the internal transcribed spacer (ITS) region is also provided.


Phytotaxa ◽  
2015 ◽  
Vol 208 (2) ◽  
pp. 147 ◽  
Author(s):  
Asanka Ranjana Bandara ◽  
Jie Chen ◽  
Samantha Karunarathna ◽  
Kevin D. Hyde ◽  
Pattana Kakumyan

Auricularia is an important genus among the jelly fungi due to its popular consumption and medicinal properties. A new species of Auricularia, A. thailandica is described from fresh collections made from the Philippines, Thailand and Southern China based on morphological and molecular characters. Auricularia thailandica differs from other species by having short and loosely arranged abhymenial hairs on the basidiomata and in the different size of the zones in a cross section of the basidiomata. The species is found to be widely distributed in Southeastern Asia. Phylogenetic relationships were inferred based on the nuclear ribosomal internal transcribed spacer (ITS) region. The new species is introduced with full description and illustrations.


Phytotaxa ◽  
2015 ◽  
Vol 203 (2) ◽  
pp. 169 ◽  
Author(s):  
Samina Sarwar ◽  
MALKA SABA ◽  
ABDUL N. KHALID ◽  
BRYN M. DENTINGER

Suillus marginielevatus sp. nov. and S. triacicularis are reported from Himalayan moist temperate forests of Pakistan in association with conifers. Morphologically S. marginielevatus is close to S. sibiricus, S. granulatus and S. intermedius but it can be distinguished by uplifted pileus margin and curved stipe with no ring at all stages. Phylogenetic analysis of internal transcribed spacer (ITS) region showed that this species form separate clade from other closely related Suillus species. Suillus triacicularis represents a new record for Pakistani mycobiota. Field photographs of fresh basidocarps and line drawings of microcharacters are provided along with phylogenetic tree.


2021 ◽  
Vol 47 (2) ◽  
Author(s):  
Daniela Cano-Calle ◽  
Clara Inés Saldamando-Benjumea ◽  
Claudia Ximena Moreno-Herrera ◽  
Rafael Eduardo Arango-Isaza

Thrips are important pests of avocados causing damage to the fruits, making themunsuitable for exportation. Many of them are quarantine species and their identification isnecessary. Despite their importance, not many studies describe thrips diversity on avocado,especially in Colombia, partly because identification of some of the species is difficult usingclassical taxonomic keys. In this study, 93 adult thrips specimens were collected on avocadoand adventitious dandelion plants from plantations in the department of Antioquia, Colombia. Morphological characters were used to classify 62 of them and all of them were analyzed by PCR amplification and sequencing of cytochrome c oxidase subunit I (COI) gene and internal transcribed spacer (ITS) region. The species identified by morphological features were Frankliniella gardeniae Moulton, F. gossypiana Hood, F. panamensis Hood, Liothrips perseae Watson, Thrips palmi Karny, T. simplex (Morison), T. trehernei Priesner, Microcephalothrips abdominalis (Crawford DL), and Scirtothrips hansoni Mound & Hoddle (Tubulifera), being F. panamensis, M. abdominalis, and S. hansoni reported for the first time on avocado in Colombia. Thrips simplex and T. trehernei were also found on adventitious dandelion plants, but not on avocado. Phylogenetic analysis of COI and ITS sequences showed that most species, except for F. gardeniae and F. gossypiana were mainly identified with the COI gene. Furthermore, FST values obtained between them suggest no genetic differentiation at COI level. The results obtained here contribute to the knowledge of the diversity of thrips species found on the avocado crop in Colombia and are meaningful for pest management.


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