Approaching Sites of Action of Temozolomide for Pharmacological and Clinical Studies in Glioblastoma

Temozolomide (TMZ), together with bulk resection and focal radiotherapy, is currently a standard of care for glioblastoma. Absorption, distribution, metabolism, and excretion (ADME) parameters, together with the mode of action of TMZ, make its biochemical and biological action difficult to understand. Accurate understanding of the mode of action of TMZ and the monitoring of TMZ at its anatomical, cellular, and molecular sites of action (SOAs) would greatly benefit precision medicine and the development of novel therapeutic approaches in combination with TMZ. In the present perspective article, we summarize the known ADME parameters and modes of action of TMZ, and we review the possible methodological options to monitor TMZ at its SOAs. We focus our descriptions of methodologies on mass spectrometry-based approaches, and all related considerations are taken into account regarding the avoidance of artifacts in mass spectrometric analysis during sampling, sample preparation, and the evaluation of results. Finally, we provide an overview of potential applications for precision medicine and drug development.

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Current and Future Treatments for Classic Galactosemia

Journal of Personalized Medicine ◽

10.3390/jpm11020075 ◽

2021 ◽

Vol 11 (2) ◽

pp. 75 ◽

Cited By ~ 1

Author(s):

Britt Delnoy ◽

Ana I. Coelho ◽

Maria Estela Rubio-Gozalbo

Keyword(s):

Standard Of Care ◽

Type I ◽

Therapeutic Approaches ◽

Restricted Diet ◽

Galactose Metabolism ◽

Galt Activity ◽

Classic Galactosemia ◽

Pathogenic Factors ◽

Future Treatments ◽

Novel Therapeutic

Type I (classic) galactosemia, galactose 1-phosphate uridylyltransferase (GALT)-deficiency is a hereditary disorder of galactose metabolism. The current therapeutic standard of care, a galactose-restricted diet, is effective in treating neonatal complications but is inadequate in preventing burdensome complications. The development of several animal models of classic galactosemia that (partly) mimic the biochemical and clinical phenotypes and the resolution of the crystal structure of GALT have provided important insights; however, precise pathophysiology remains to be elucidated. Novel therapeutic approaches currently being explored focus on several of the pathogenic factors that have been described, aiming to (i) restore GALT activity, (ii) influence the cascade of events and (iii) address the clinical picture. This review attempts to provide an overview on the latest advancements in therapy approaches.

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Tandem mass spectrometric analysis of Aspergillus niger pectin methylesterase: mode of action on fully methyl-esterified oligogalacturonates

Biochemical Journal ◽

10.1042/0264-6021:3460469 ◽

2000 ◽

Vol 346 (2) ◽

pp. 469 ◽

Cited By ~ 8

Author(s):

Harry C.M. KESTER ◽

Jacques A.E. BENEN ◽

Jaap VISSER ◽

Maria Esteban WARREN ◽

Ron ORLANDO ◽

...

Keyword(s):

Aspergillus Niger ◽

Mode Of Action ◽

Pectin Methylesterase ◽

Mass Spectrometric ◽

Mass Spectrometric Analysis ◽

Spectrometric Analysis ◽

Tandem Mass ◽

Tandem Mass Spectrometric Analysis ◽

Tandem Mass Spectrometric

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Precision medicine for gastrointestinal cancers: a conference report

Future Science OA ◽

10.2144/fsoa-2020-0061 ◽

2020 ◽

Vol 6 (6) ◽

pp. FSO478

Author(s):

Ibrahim Halil Sahin

Keyword(s):

Clinical Trials ◽

Precision Medicine ◽

Conference Report ◽

Daily Practice ◽

Future Perspective ◽

Gastrointestinal Cancers ◽

Therapeutic Approaches ◽

Important Data ◽

Cancer Management ◽

Novel Therapeutic

As cancer management evolves into precision medicine national/international cancer meetings bring novel therapeutic approaches and potentially practice-changing results of clinical studies are presented. This year, the ASCO GI Symposium 2020 had also several updates from ongoing and finalized clinical trials. Although there were no groundbreaking results that impact the daily practice directly, several highly important data from ongoing studies were shared with the audience. In this report, the highlights of the ASCO GI Symposium 2020 are presented with a future perspective.

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