scholarly journals Nanoribbon-Based Electronic Detection of a Glioma-Associated Circular miRNA

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 237
Author(s):  
Yuri D. Ivanov ◽  
Kristina A. Malsagova ◽  
Vladimir P. Popov ◽  
Tatyana O. Pleshakova ◽  
Andrey F. Kozlov ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Molecular Marker ◽  
Ribonucleic Acid ◽  
Elevated Level ◽  
Circular Rna ◽  
Molecular Probes ◽  
Silicon On Insulator ◽  
Synthetic Analogue ◽  
Glioma Patient ◽  
Electronic Detection

Nanoribbon chips, based on “silicon-on-insulator” structures (SOI-NR chips), have been fabricated. These SOI-NR chips, whose surface was sensitized with covalently immobilized oligonucleotide molecular probes (oDNA probes), have been employed for the nanoribbon biosensor-based detection of a circular ribonucleic acid (circRNA) molecular marker of glioma in humans. The nucleotide sequence of the oDNA probes was complimentary to the sequence of the target oDNA. The latter represents a synthetic analogue of a glioma marker—NFIX circular RNA. In this way, the detection of target oDNA molecules in a pure buffer has been performed. The lowest concentration of the target biomolecules, detectable in our experiments, was of the order of ~10−17 M. The SOI-NR sensor chips proposed herein have allowed us to reveal an elevated level of the NFIX circular RNA in the blood of a glioma patient.

scholarly journals SOI-Nanowire Biosensor for the Detection of Glioma-Associated miRNAs in Plasma

Chemosensors ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 95
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Rafael A. Galiullin ◽  
Andrey F. Kozlov ◽  
Tatyana S. Romanova ◽  
...  
Keyword(s):  
Elevated Level ◽  
Silicon Nanowire ◽  
Silicon On Insulator ◽  
Sensor Chip ◽  
Concentration Limit ◽  
Synthetic Analogue ◽  
Oligonucleotide Probes ◽  
System A ◽  
Highly Sensitive ◽  
Oligonucleotide Detection

Herein, we report the development of a highly sensitive nanotechnology-based system—silicon-on-insulator nanowire biosensor for the revelation of microRNAs (miRNAs), associated with the development of glioma in the human. In this system, a sensor chip, bearing an array of silicon nanowire structures, is employed. The sensor chip is fabricated using a top-down technology. In our experiments reported herein, we demonstrated the detection of DNA oligonucleotide (oDNA), which represents a synthetic analogue of microRNA-363 associated with the development of glioma. To provide biospecific detection of the target oligonucleotides, the surface of the nanowire structures is modified with oligonucleotide probes; the latter are complementary to the target ones. The concentration limit of the target oligonucleotide detection, attained using our nanowire biosensor, is at the level of DL~10−17 M. The revelation of the elevated level of glioma-associated miRNA in plasma is also demonstrated.

scholarly journals Nucleotide sequence of an arginine transfer ribonucleic acid from bacteriophage T4.

1977 ◽  
Vol 252 (22) ◽  
pp. 8245-8253 ◽  
Author(s):  
G.P. Mazzara ◽  
J.G. Seidman ◽  
W.H. McClain ◽  
H. Yesian ◽  
J. Abelson ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Bacteriophage T4

scholarly journals The primary nucleotide sequence of nuclear U-2 ribonucleic acid. The 5'-terminal portion of the molecule.

1975 ◽  
Vol 250 (10) ◽  
pp. 3909-3920 ◽  
Author(s):  
H Shibata ◽  
TS Ro-Choi ◽  
R Reddy ◽  
YC Choi ◽  
D Henning ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Terminal Portion ◽  
Primary Nucleotide Sequence

scholarly journals The Nucleotide Sequence of the GGG-specific Glycine Transfer Ribonucleic Acid of Escherichia coli and of Salmonella typhimurium

1973 ◽  
Vol 248 (12) ◽  
pp. 4252-4262
Author(s):  
C.W. Hill ◽  
Gabriele Combriato ◽  
William Steinhart ◽  
Donald L. Riddle ◽  
John Carbon
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Salmonella Typhimurium ◽  
Ribonucleic Acid

Nucleotide sequence from the 5' end to the first cistron of R17 bacteriophage ribonucleic acid

Biochemistry ◽  
1972 ◽  
Vol 11 (6) ◽  
pp. 976-988 ◽  
Author(s):  
Jerry M. Adams ◽  
Pierre F. Spahr ◽  
Suzanne Cory
Keyword(s):  
Nucleotide Sequence ◽  
Ribonucleic Acid

scholarly journals The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Products of complete digestion with pancreatic ribonuclease and ribonuclease T1

1976 ◽  
Vol 153 (2) ◽  
pp. 437-446 ◽  
Author(s):  
N J Holness ◽  
G Atfield
Keyword(s):  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Brief Treatment ◽  
Ribonuclease T1 ◽  
Pancreatic Ribonuclease ◽  
Adenylic Acid ◽  
Complete Digestion ◽  
Conventional Methods

1. The nucleotide chain of tRNA Cys from baker's yeast was readily split at the anticolon into two large fragments by brief treatment with ribonuclease T1.2. The whole molecule and the two derived large fragments were completely digested with (a) pancreatic ribonuclease and (b) ribonuclease T1. The fragments present in each of the digests were separated and sequenced by conventional methods. 3. The groups of fragments derived from the two methods of digestion were entirely compatible with each other. 4. The molecule is 75 nucleotides long, but, as isolated, lacks the terminal adenosine and the neighboring cytidylic acid residue. The minor nucleotides 1-methyladenylic acid, 7-methylguanylic acid, 5-methylcytidylic acid and N6 (γγ-dimethylallyl)adenylic acid (isopentenyladenylic acid) were identified.

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