Reagentless D-Tagatose Biosensors Based on the Oriented Immobilization of Fructose Dehydrogenase onto Coated Gold Nanoparticles- or Reduced Graphene Oxide-Modified Surfaces: Application in a Prototype Bioreactor

As electrode nanomaterials, thermally reduced graphene oxide (TRGO) and modified gold nanoparticles (AuNPs) were used to design bioelectrocatalytic systems for reliable D-tagatose monitoring in a long-acting bioreactor where the valuable sweetener D-tagatose was enzymatically produced from a dairy by-product D-galactose. For this goal D-fructose dehydrogenase (FDH) from Gluconobacter industrius immobilized on these electrode nanomaterials by forming three amperometric biosensors: AuNPs coated with 4-mercaptobenzoic acid (AuNP/4-MBA/FDH) or AuNPs coated with 4-aminothiophenol (AuNP/PATP/FDH) monolayer, and a layer of TRGO on graphite (TRGO/FDH) were created. The immobilized FDH due to changes in conformation and spatial orientation onto proposed electrode surfaces catalyzes a direct D-tagatose oxidation reaction. The highest sensitivity for D-tagatose of 0.03 ± 0.002 μA mM–1cm–2 was achieved using TRGO/FDH. The TRGO/FDH was applied in a prototype bioreactor for the quantitative evaluation of bioconversion of D-galactose into D-tagatose by L-arabinose isomerase. The correlation coefficient between two independent analyses of the bioconversion mixture: spectrophotometric and by the biosensor was 0.9974. The investigation of selectivity showed that the biosensor was not active towards D-galactose as a substrate. Operational stability of the biosensor indicated that detection of D-tagatose could be performed during six hours without loss of sensitivity.

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Sponge-templating synthesis of sandwich-like reduced graphene oxide nanoplates with confined gold nanoparticles and their enhanced stability for solar evaporation

Science China Materials ◽

10.1007/s40843-020-1446-5 ◽

2020 ◽

Vol 63 (10) ◽

pp. 1957-1965 ◽

Cited By ~ 1

Author(s):

Hong-Wu Zhu ◽

Jin Ge ◽

Hao-Yu Zhao ◽

Lu-An Shi ◽

Jin Huang ◽

...

Keyword(s):

Gold Nanoparticles ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Reduced Graphene ◽

Templating Synthesis ◽

Enhanced Stability

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Gold nanoparticles decorated silicate sol-gel matrix embedded reduced graphene oxide and manganese ferrite nanocomposite-materials-modified electrode for glucose sensor application

Journal of Chemical Sciences ◽

10.1007/s12039-019-1611-z ◽

2019 ◽

Vol 131 (5) ◽

Cited By ~ 1

Author(s):

T Ravindran Madhura ◽

G Gnana Kumar ◽

Ramasamy Ramaraj

Keyword(s):

Gold Nanoparticles ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Modified Electrode ◽

Glucose Sensor ◽

Sol Gel ◽

Nanocomposite Materials ◽

Manganese Ferrite ◽

Sensor Application ◽

Reduced Graphene

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Layer-by-layer assembled multilayer films of reduced graphene oxide/gold nanoparticles for the electrochemical detection of dopamine

Journal of Electroanalytical Chemistry ◽

10.1016/j.jelechem.2012.03.007 ◽

2012 ◽

Vol 672 ◽

pp. 40-44 ◽

Cited By ~ 114

Author(s):

Shi Liu ◽

Jun Yan ◽

Guangwu He ◽

Dandan Zhong ◽

Jiaxing Chen ◽

...

Keyword(s):

Gold Nanoparticles ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Electrochemical Detection ◽

Multilayer Films ◽

Layer By Layer ◽

Reduced Graphene

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Prussian blue–gold nanoparticles-ionic liquid functionalized reduced graphene oxide nanocomposite as label for ultrasensitive electrochemical immunoassay of alpha-fetoprotein

Analytica Chimica Acta ◽

10.1016/j.aca.2014.04.045 ◽

2014 ◽

Vol 829 ◽

pp. 15-21 ◽

Cited By ~ 28

Author(s):

Qi Gao ◽

Na Liu ◽

Zhanfang Ma

Keyword(s):

Gold Nanoparticles ◽

Ionic Liquid ◽

Graphene Oxide ◽

Prussian Blue ◽

Reduced Graphene Oxide ◽

Alpha Fetoprotein ◽

Electrochemical Immunoassay ◽

Reduced Graphene

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A simple immunosensor for alpha-fetoprotein determination based on gold nanoparticles-dextran-reduced graphene oxide

Journal of Electroanalytical Chemistry ◽

10.1016/j.jelechem.2018.11.036 ◽

2019 ◽

Vol 833 ◽

pp. 126-132 ◽

Cited By ~ 3

Author(s):

Jiexin Zhou ◽

Cong Zhang ◽

Yuan Chen ◽

Zihua Wang ◽

Lintao Lan ◽

...

Keyword(s):

Gold Nanoparticles ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Alpha Fetoprotein ◽

Reduced Graphene

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Hg(II) Ion Detection Using Thermally Reduced Graphene Oxide Decorated with Functionalized Gold Nanoparticles

Analytical Chemistry ◽

10.1021/ac3000336 ◽

2012 ◽

Vol 84 (9) ◽

pp. 4057-4062 ◽

Cited By ~ 156

Author(s):

Kehung Chen ◽

Ganhua Lu ◽

Jingbo Chang ◽

Shun Mao ◽

Kehan Yu ◽

...

Keyword(s):

Gold Nanoparticles ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Ion Detection ◽

Functionalized Gold Nanoparticles ◽

Reduced Graphene

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Electrodeposition–Assisted Assembled Multilayer Films of Gold Nanoparticles and Glucose Oxidase onto Polypyrrole-Reduced Graphene Oxide Matrix and Their Electrocatalytic Activity toward Glucose

Nanomaterials ◽

10.3390/nano8120993 ◽

2018 ◽

Vol 8 (12) ◽

pp. 993 ◽

Cited By ~ 3

Author(s):

Baoyan Wu ◽

Shihua Hou ◽

Yongyong Xue ◽

Zhan Chen

Keyword(s):

Gold Nanoparticles ◽

Graphene Oxide ◽

Glucose Oxidase ◽

Reduced Graphene Oxide ◽

Self Assembly ◽

Electrocatalytic Activity ◽

Multilayer Films ◽

Glucose Biosensor ◽

Future Design ◽

Reduced Graphene

The study reports a facile and eco-friendly approach for nanomaterial synthesis and enzyme immobilization. A corresponding glucose biosensor was fabricated by immobilizing the gold nanoparticles (AuNPs) and glucose oxidase (GOD) multilayer films onto the polypyrrole (PPy)/reduced graphene oxide (RGO) modified glassy carbon electrode (GCE) via the electrodeposition and self-assembly. PPy and graphene oxide were first coated on the surface of a bare GCE by the electrodeposition. Then, AuNPs and GOD were alternately immobilized onto PPy-RGO/GCE electrode using the electrodeposition of AuNPs and self-assembly of GOD to obtain AuNPs-GOD multilayer films. The resulting PPy-RGO-(AuNPs-GOD)n/GCE biosensors were used to characterize and assess their electrocatalytic activity toward glucose using cyclic voltammetry and amperometry. The response current increased with the increased number of AuNPs-GOD layers, and the biosensor based on four layers of AuNPs-GOD showed the best performance. The PPy-RGO-(AuNPs-GOD)4/GCE electrode can detect glucose in a linear range from 0.2 mM to 8 mM with a good sensitivity of 0.89 μA/mM, and a detection limit of 5.6 μM (S/N = 3). This study presents a promising eco-friendly biosensor platform with advantages of electrodeposition and self-assembly, and would be helpful for the future design of more complex electrochemical detection systems.

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Layer-by-layer thin film of reduced graphene oxide and gold nanoparticles as an effective sample plate in laser-induced desorption/ionization mass spectrometry

Analytica Chimica Acta ◽

10.1016/j.aca.2013.11.050 ◽

2014 ◽

Vol 809 ◽

pp. 97-103 ◽

Cited By ~ 23

Author(s):

Tsung-Rong Kuo ◽

Di-Yan Wang ◽

Yu-Chen Chiu ◽

Yun-Chieh Yeh ◽

Wei-Ting Chen ◽

...

Keyword(s):

Thin Film ◽

Mass Spectrometry ◽

Gold Nanoparticles ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Ionization Mass ◽

Layer By Layer ◽

Desorption Ionization ◽

Reduced Graphene ◽

Layer Thin Film

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Reduced Graphene Oxide/Gold Nanoparticles Modified Screen-Printed Electrode for the Determination of Palmitic Acid

Journal of Sensors ◽

10.1155/2021/6684770 ◽

2021 ◽

Vol 2021 ◽

pp. 1-14

Author(s):

Chin Boon Ching ◽

Jaafar Abdullah ◽

Nor Azah Yusof

Keyword(s):

Gold Nanoparticles ◽

Graphene Oxide ◽

Palmitic Acid ◽

Reduced Graphene Oxide ◽

Palm Oil ◽

Water Mixture ◽

Reduced Graphene ◽

Electrochemical Approach ◽

Screen Printed

Palm oil is one of the major oils and fats produced in the world today. The quality of palm oil is crucial to be investigated, and one of the quality indices is free fatty acid (FFA) content. Therefore, in this study, an electrochemical approach for the determination of FFA has been explored as an alternative to replace the conventional method (titration method). The electrochemical method was developed based on electrochemically reduced graphene oxide (rGO) coupled with gold nanoparticles (AuNPs) deposited onto a screen-printed carbon electrode (SPCE) via drop-casting technique. The voltammetric behaviour of 2-methyl-1,4-naphthoquinone (VK3) in the presence of palmitic acid at the modified electrode was investigated in an acetonitrile/water mixture containing lithium perchlorate (LiClO4). The electrochemical detection of palmitic acid was based on the voltammetric reduction of VK3 to form the corresponding hydroquinone which is proportional to the concentration of palmitic acid. Under optimum conditions, the developed method showed a good linear relationship towards palmitic acid in the concentration ranging from 0.192 mM to 0.833 mM with the detection limit of 0.015 mM. The exploration of the developed system is expected to achieve high sensitivity and excellent selectivity towards the determination of FFA content in palm oil.

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