scholarly journals Drosophila HUWE1 Ubiquitin Ligase Regulates Endoreplication and Antagonizes JNK Signaling During Salivary Gland Development

Cells ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. 151 ◽  
Author(s):  
Yifat Yanku ◽  
Eliya Bitman-Lotan ◽  
Yaniv Zohar ◽  
Estee Kurant ◽  
Norman Zilke ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Ubiquitin Ligase ◽  
Essential Gene ◽  
Inhibitory Effect ◽  
Jnk Signaling ◽  
Myc Oncogene ◽  
Survival Factor ◽  
Salivary Gland Cells ◽  
Larval Salivary Gland ◽  
Gland Development

The HECT-type ubiquitin ligase HECT, UBA and WWE Domain Containing 1, (HUWE1) regulates key cancer-related pathways, including the Myc oncogene. It affects cell proliferation, stress and immune signaling, mitochondria homeostasis, and cell death. HUWE1 is evolutionarily conserved from Caenorhabditis elegance to Drosophila melanogaster and Humans. Here, we report that the Drosophila ortholog, dHUWE1 (CG8184), is an essential gene whose loss results in embryonic lethality and whose tissue-specific disruption establishes its regulatory role in larval salivary gland development. dHUWE1 is essential for endoreplication of salivary gland cells and its knockdown results in the inability of these cells to replicate DNA. Remarkably, dHUWE1 is a survival factor that prevents premature activation of JNK signaling, thus preventing the disintegration of the salivary gland, which occurs physiologically during pupal stages. This function of dHUWE1 is general, as its inhibitory effect is observed also during eye development and at the organismal level. Epistatic studies revealed that the loss of dHUWE1 is compensated by dMyc proeitn expression or the loss of dmP53. dHUWE1 is therefore a conserved survival factor that regulates organ formation during Drosophila development.

Rickettsiae-like structures in the larval salivary gland cells ofDrosophila auraria

1991 ◽  
Vol 207 (1) ◽  
pp. 17-21 ◽  
Author(s):  
George N. Thomopoulos ◽  
Elefterios P. Neophytou ◽  
Stella Limberi-Thomopoulos
Keyword(s):  
Salivary Gland ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Larval Salivary Gland

scholarly journals Nucleocytoplasmic movement of fluorescent tracers microinjected into living salivary gland cells.

1975 ◽  
Vol 66 (3) ◽  
pp. 652-657 ◽  
Author(s):  
P L Paine
Keyword(s):  
Salivary Gland ◽  
Conformational Changes ◽  
Morphological Evidence ◽  
Salivary Gland Cell ◽  
Naturally Occurring ◽  
Salivary Gland Cells ◽  
Larval Salivary Gland ◽  
Pore Complexes ◽  
Nuclear Envelop

The permeability of the nuclear envelop of a somatic cell, the C. thummi larval salivary gland cell, was studied by intracellular microinjection of fluorescent molecular tracers. As shown previously in oocytes (4,5,15,16), the envelop is permeable to a wide variety of materials, including molecules which are large enough to possess condiderable biological specificities and to play important roles in regulation of cellular activities. The envelop exhibits transport selectivity on the basis of size in the range of naturally occurring intracellular materials and it may thus perform important controlling functions in nucleocytoplasmic exchange. The nucleus to cytoplasm movement of in vivo ribonucleoprotein particulates in these synthetically active cells probably requires conformational changes in the particulates and/or the envelope pore complexes; morphological evidence exists for such processess in these cells (20).

scholarly journals AnIn VitroCulture System for Long-Term Expansion of Epithelial and Mesenchymal Salivary Gland Cells: Role of TGF-β1 in Salivary Gland Epithelial and Mesenchymal Differentiation

2013 ◽  
Vol 2013 ◽  
pp. 1-20 ◽  
Author(s):  
Kajohnkiart Janebodin ◽  
Worakanya Buranaphatthana ◽  
Nicholas Ieronimakis ◽  
Aislinn L. Hays ◽  
Morayma Reyes
Keyword(s):  
Salivary Gland ◽  
Culture System ◽  
Gland Cell ◽  
Mesenchymal Cells ◽  
Salivary Gland Cell ◽  
Salivary Gland Cells ◽  
Gland Development

Despite a pivotal role in salivary gland development, homeostasis, and disease, the role of salivary gland mesenchyme is not well understood. In this study, we used theCol1a1-GFPmouse model to characterize the salivary gland mesenchymein vitroandin vivo. TheCol1a1-GFPtransgene was exclusively expressed in the salivary gland mesenchyme.Ex vivoculture of mixed salivary gland cells in DMEM plus serum medium allowed long-term expansion of salivary gland epithelial and mesenchymal cells. The role of TGF-β1 in salivary gland development and disease is complex. Therefore, we used thisin vitroculture system to study the effects of TGF-β1 on salivary gland cell differentiation. TGF-β1 induced the expression of collagen, and inhibited the formation of acini-like structures in close proximity to mesenchymal cells, which adapted a fibroblastic phenotype. In contrast, TGF-βR1 inhibition increased acini genes and fibroblast growth factors (Fgf-7andFgf-10), decreased collagen and induced formation of larger, mature acini-like structures. Thus, inhibition of TGF-βsignaling may be beneficial for salivary gland differentiation; however, due to differential effects of TGF-β1 in salivary gland epithelial versus mesenchymal cells, selective inhibition is desirable. In conclusion, this mixed salivary gland cell culture system can be used to study epithelial-mesenchymal interactions and the effects of differentiating inducers and inhibitors.

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