Characterisation and enzymatic profiling of a halotolerant <em>Penicillium</em> strain

AbstractThe humanKLK14gene is one of the newly identified serine protease genes belonging to the human kallikrein family, which contains 15 members.KLK14, like all other members of the human kallikrein family, is predicted to encode for a secreted serine protease already found in various biological fluids. This new kallikrein is mainly expressed in prostate and endocrine tissues, but its function is still unknown. Recent studies have demonstrated thatKLK14gene expression is up-regulated in prostate and breast cancer tissues, and that higher expression levels correlate with more aggressive tumors. In this work, we used phage-display substrate technology to study the substrate specificity of hK14. A phage-displayed random pentapeptide library with exhaustive diversity was screened with purified recombinant hK14. Highly specific and sensitive substrates were selected from the library. We show that hK14 has dual activity, trypsin- and chymotrypsin-like, with a preference for cleavage after arginine residues. A SwissProt database search with selected sequences identified six potential human protein substrates for hK14. Two of them, laminin α-5 and collagen IV, which are major components of the extracellular matrix, have been demonstrated to be hydrolyzed efficiently by hK14.

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Phenotypic and molecular characterization of a novel strongly hemolytic Brachyspira species, provisionally designated “Brachyspira hampsonii”

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638712456975 ◽

2012 ◽

Vol 24 (5) ◽

pp. 903-910 ◽

Cited By ~ 61

Author(s):

Yogesh Chander ◽

Alexander Primus ◽

Simone Oliveira ◽

Connie J. Gebhart

Keyword(s):

United States ◽

Phylogenetic Analyses ◽

The United States ◽

Rrna Genes ◽

Rrna Gene ◽

Diagnostic Laboratory ◽

Brachyspira Hyodysenteriae ◽

Phylogenetic Grouping ◽

Enzymatic Profiling ◽

Swine Herds

Since 2007, outbreaks of severe bloody diarrhea and hemorrhagic colitis have been reported in the United States and Canada. Though the primary causative agent of swine dysentery is Brachyspira hyodysenteriae, which is strongly hemolytic, the current report describes the isolation of a novel strongly hemolytic Brachyspira sp. This novel Brachyspira sp. was identified from clinical submissions at the Minnesota Veterinary Diagnostic Laboratory, and 40 of such isolates were obtained from 22 clinical submissions representing 5 states. Isolates were confirmed to be different from any known Brachyspira sp. on the basis of phylogenetic analysis of nucleotide sequences of nox and 16S ribosomal RNA (rRNA) genes. Phylogenetic analyses grouped all isolates into 2 clades (clades I and II), and grouping patterns were similar for both nox and 16S rRNA gene sequence analyses. Phenotypically, all isolates were indole and hippurate negative, and enzymatic profiling indicated 2 types of profiles, irrespective of the phylogenetic grouping, differing only in the production of β-glucosidase. The results suggest that a potentially virulent new species of Brachyspira sp., provisionally named “ Brachyspira hampsonii ”, is circulating among swine herds in the United States.

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Biochemical Characterization and Enzymatic profiling of Potential Probiotic Yeast Strains

Research Journal of Pharmacy and Technology ◽

10.5958/0974-360x.2019.00679.6 ◽

2019 ◽

Vol 12 (8) ◽

pp. 3941 ◽

Cited By ~ 1

Author(s):

Mangala Lakshmi Ragavan ◽

Niharika Patnaik ◽

Rajeshwari Muniyasamy ◽

Arpita Roy ◽

Loknath Deo ◽

...

Keyword(s):

Biochemical Characterization ◽

Yeast Strains ◽

Probiotic Yeast ◽

Enzymatic Profiling

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Diversity and Enzymatic Profiling of Halotolerant Micromycetes from Sebkha El Melah, a Saharan Salt Flat in Southern Tunisia

BioMed Research International ◽

10.1155/2014/439197 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 11

Author(s):

Atef Jaouani ◽

Mohamed Neifar ◽

Valeria Prigione ◽

Amani Ayari ◽

Imed Sbissi ◽

...

Keyword(s):

Internal Transcribed Spacer ◽

Enzyme Production ◽

Rrna Gene ◽

28S Rrna ◽

Liquid Media ◽

Oxidative Stresses ◽

Salt Flat ◽

Southern Tunisia ◽

Initial Ph ◽

Enzymatic Profiling

Twenty-one moderately halotolerant fungi have been isolated from sample ashes collected from Sebkha El Melah, a Saharan salt flat located in southern Tunisia. Based on morphology and sequence inference from the internal transcribed spacer regions, 28S rRNA gene and other specific genes such asβ-tubulin, actin, calmodulin, and glyceraldehyde-3-phosphate dehydrogenase, the isolates were found to be distributed over 15 taxa belonging to 6 genera of Ascomycetes:Cladosporium(n=3),Alternaria(n=4),Aspergillus(n=3),Penicillium(n=5),Ulocladium(n=2), andEngyodontium(n=2). Their tolerance to different concentrations of salt in solid and liquid media was examined. ExceptingCladosporium cladosporioidesJA18, all isolates were considered as alkali-halotolerant since they were able to grow in media containing 10% of salt with an initial pH 10. All isolates were resistant to oxidative stresses and low temperature whereas 5 strains belonging toAlternaria,Ulocladium,andAspergillusgenera were able to grow at 45°C. The screening of fungal strains for sets of enzyme production, namely, cellulase (CMCase), amylase, protease, lipase, and laccase, in presence of 10% NaCl, showed a variety of extracellular hydrolytic and oxidative profiles. Protease was the most abundant enzyme produced whereas laccase producers were members of the genusCladosporium.

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