Genome Sequence of Halobacterium sp. Strain BOL4-2, Isolated and Cultured from Salar de Uyuni, Bolivia

Halobacterium sp. strain BOL4-2 was isolated from an Andean salt flat, Salar de Uyuni, in Bolivia. Single-molecule real-time (SMRT) sequencing revealed a 2.4-Mbp genome with a 2.0-Mbp chromosome and four plasmids (2 to 299 kb). Its isolation from an environment experiencing multiple extremes makes the strain interesting for astrobiology.

Differentiation of Diatom Guilds in Extreme Environments in the Andean Altiplano

The Andean Altiplano is characterized by its isolation, high altitude (>3,600 m a.s.l.), and a unique biodiversity, containing water systems such as lakes, rivers, wetlands, and salt flats. Near the southernmost area of the Andean Altiplano are two salt flats, Carcote and Ascotán, the former almost undisturbed by human intervention and the latter historically intervened with by brine mining. These mining activities deplete, pollute, and alter water flow, suggesting a significant threat to ecosystem functions. With regard to identifying the main factors promoting biotic community differentiation in these salt flats at different levels of human intervention, the aim of this study was to compare diatom community indices by guild (high-profile, low-profile, and motile) between these systems with a functional perspective, their relationship to environmental conditions, and the potential anthropic impact on their community structures. Diatom guilds were compared between salt flats based on their diversity, species richness, and abundance relative to ionic concentrations and granulometry. Beta diversity and the percentages of nestedness and turnover for each guild were also estimated. Results showed significant differences in ecological variables between the two salt flats. The largest values of species abundance, richness, and diatom diversity were measured in Carcote, suggesting greater primary productivity and diversity. The results also showed that the species composition of the motile guild was more differentiated between salt flats than those of other guilds (with the greatest diversity and richness), suggesting that motile diatoms are a key guild in maintaining the diatom community and that species from this guild are more sensitive to local conditions from each salt flat. Additionally, beta diversity in all guilds was mainly explained by turnover rather than nestedness, and the turnover was the highest for the motile and low-profile guilds. A more heterogeneous community was observed in Ascotán, showing that the mechanisms of diversity maintenance, such as dispersion abilities, were salt flat dependent. This suggests that the highest diversity could be related to human use, which challenges us to reexamine the effectiveness of past conservation activities in the area and to develop future strategies including both connected basins.

Novosphingobium aureum sp. nov., a marine bacterium isolated from salt flat sediment

A Gram-stain-negative, aerobic, pale yellow-coloured, rod-shaped marine bacterium designated strain YJ-S2-02T was isolated from salt flat sediment sampled in Yongyu-do, Republic of Korea. Strain YJ-S2-02T grew at pH 6.0–9.0 (optimum, pH 7.0), 10–40 °C (optimum, 30 °C) and with optimum 1 % (w/v) NaCl. The 16S rRNA gene sequence analysis indicated that strain YJ-S2-02T was closely related to Novosphingobium naphthalenivorans NBRC 102051T (97.8 %) followed by Novosphingobium mathurense SM117T (97.5 %), Novosphingobium indicum H25T (97.3 %), Novosphingobium pentaromativorans US6-1T (96.8 %), Novosphingobium fontis STM-14T (96.6 %), Novosphingobium endophyticum EGI60015T (96.5 %), Novosphingobium naphthae D39T (96.5 %) and Novosphingobium malaysiense MUSC 273T (95.9 %). The average nucleotide identity and estimated DNA–DNA hybridization values between YJ-S2-02T and related type strains were 77.0–77.9 % and 19.1–24.0 %. Strain YJ-S2-02T was characterized as having Q-10 as the predominant respiratory quinone and the principal fatty acids (>10 %) were summed feature 8 (C18 : 1 ω6c/ω7c, 20.7 %), C18 : 3 ω6c (16.3 %) and C17 : 1 ω6c (11.8 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingolipids and two unidentified lipids. The DNA G+C content of strain YJ-S2-02T was 65.6 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S2-02T should be classified as representing a novel species within the genus Novosphingobium , for which name Novosphingobium aureum is proposed, with the type strain YJ-S2-02T (=KACC 21677T =KCTC 72891T=JCM 33996T).

Las Quínoas oncoids: a new deposit of microbialites in the Salar de Antofalla (Catamarca, Argentina)

The Salar de Antofalla (salt flat) is located in the Puna region of Catamarca, in northern Argentina. In this paper we report and provide the first descriptive data of Las Quínoas, a modern system of oncoids located in the western margin of the salt flat. Oncoids were studied by insitu logging, polished and thin sections analysis. In addition, the 16s rRNA genes of microbial mats associated with these oncoids were amplified and sequenced to characterize the microbial biodiversity. Oncoids present discoidal to subrounded morphologies and sizes up to 15 cm in diameter. They are scattered along channels, which originate from the groundwater springs of a wetland and enter the salt flat. Its macrostructure is concentric and composed by three zones: 1. A nucleus zone built by clastic material in a carbonate matrix. 2. A well-laminated zone around the nucleus that shows two types of mesostructures: concentrically stacked spheroids (SS-C) and randomly stacked hemispheroids (SS-R), both showing an alternation of dense and dark micritic laminae with light micritic to microsparitic laminae. 3. A poorly-laminated zone, in the outermost sector of oncoids, with two types of mesostructures too: a laminated mesostructure composed also of an alternation of dense and dark micritic laminae with light micritic to microsparitic laminae, and a non-laminated mesostructure composed of agglomerated and cemented clastic material within a calcareous matrix (wackestones-packstones). Regarding the microbial diversity, the analyzed oncoids in this work are mainly inhabited by Proteobacteria (ca. 37.5%), Bacteroidetes (ca. 25.0%), and in less proportion Planctomycetes, Actinobacteria and Cyanobacteria.

A solution to the enigma of the type locality of Telmatobius halli Noble, 1938 (Anura: Telmatobiidae), a lost frog for 85 years

For 80 years, there were no sightings of the Andean amphibian Telmatobius halli due to the ambiguity with which its type locality was described (“warm spring near Ollagüe”, northern Chile). The type specimens were collected during the International High Altitude Expedition to Chile (IHAEC) of 1935, but they were later described in 1938. Recently, in 2018 and 2020, two studies independently reported the rediscovery of the species. Although in these studies live specimens were analyzed and bibliographic references were consulted, both reached different conclusions about its identity and distribution. The 2018 proposal identifies the populations of the Chijlla-Choja and Copaquire ravines (previously assigned to T. chusmisensis) as T. halli, whereas the 2020 proposal identifies the hot spring “Aguas Calientes”, located in the Carcote salt flat some 12 km SW of Ollagüe, as its type locality. The problem with these two proposals is that these populations are more phylogenetically related to other species than to each other, so they clearly do not belong to the same taxon. Although the last proposal is more in line with the geographic information of the description, it does not consider some bibliographic details and the transport limitations of the IHAEC. Here, based on a detailed analysis of the chronicles of the IHAEC and other bibliographic sources, I first refute the 2018 and 2020 proposals and then provide a solution: the type locality of T. halli is Miño, an abandoned mining camp located at the source of the Loa river

Using a socio-hydrology stance to address the paradox between global decarbonisation, lithium fever, and sustainability in the Atacama Salt Deposit

<p>Climate-warming greenhouse gas emissions can be reduced by replacing petroleum-driven vehicles with electric vehicles powered by rechargeable lithium batteries. By 2025, 45% of the world’s Lithium will be sourced from water-intensive mining operations adjacent to fragile eco-hydrological systems in the Atacama Desert, the world’s driest desert. In the remote Atacama salt flat basin, home to one of the world’s richest deposits of high-grade lithium, brines are being mined from aquifers, with potential impacts on the long-term environmental, ecological, economic, and social viability of the system. Stakeholders (scientists, communities, and decision-makers) are currently entrenched in adversarial relationships and top-down policy-making and implementation.</p><p>A socio-hydrology stance considering telecoupled systems of people and water is essential to address the paradox between the quest for global decarbonisation and unsustainable use of water resources in the Atacama region. The inclusion of social drivers (beliefs, biases, values, and heuristics), however, adds complexity to the analysis. To address this complexity, novel methodologies such as participatory modeling (PM) and agent-based modeling (ABM) can be implemented. The former can enrich the system with specialist and local knowledge, increase the perceived utility of models, their credibility through transparent communication of the limitations and uncertainties, and the adoption and acceptance of the model results, which ultimately guide public policy. The latter seeks to represent explicitly the complexity and heterogeneity in these telecoupled systems.</p><p>The socio-hydrological problem at the Atacama salt flat is conceptualized using the Fuzzy-Logic Cognitive Mapping methodology through participatory workshops, involving scientists, regulators, and government officials. An ABM is then coupled to an integrated and regional groundwater-surface water model to better understand the impacts of management scenarios and social interactions, and their feedbacks on the eco-hydrological system. Ultimately, the aim of this research is to take a socio-hydrology stance to analyze a wicked problem with social, environmental, and economic implications at the local and global scales, and in doing so, expand fundamental knowledge of socio-hydrology.</p>

Marinobacter halodurans sp. nov., a halophilic bacterium isolated from sediment of a salt flat

A Gram-staining-negative, aerobic, cream-coloured, marine bacterium, with rod-shaped cells, designated strain YJ-S3-2T, was isolated from salt flat sediment of Yongyu-do, Republic of Korea. YJ-S3-2T grew at pH 5.0–9.0 (optimum pH 7.0), 4–45 °C (optimum 30 °C) and with 1–18 % (w/v) NaCl (optimum 6 %). The results of 16S rRNA gene sequence analysis indicated that YJ-S3-2T was closely related to Marinobacter segnicrescens SS011B1-4T (97.0 %) followed by, ' Marinobacter nanhaiticus ' D15-8W (96.7 %), Marinobacter bryozoorum 50-11T (96.7 %), Marinobacter koreensis DSMZ 179240T T (96.5 %) and Marinobacter bohaiensis T17T (96.5 %). The average nucleotide identity (ANI) and the genome to genome distance calculator (GGDC) estimate values between YJ-S3-2T and related type strains were 73.7–79.8 and 19.9–22.5 %, and also 73.5 and 20.7 % with Marinobacter hydrocarbonoclasticus . YJ-S3-2T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10 %) were C16 : 0 (22.3 %), summed feature 9 (C17 : 1iso ω9c/C16 : 0 10-methyl, 13.8 %) and 3 (C16 : 1ω7c/C16 : 1ω6c, 11.9 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and two unidentified phospholipids. The DNA G+C content of YJ-S3-2T is 60.9 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S3-2T should be classified as representing a novel species within the genus Marinobacter , for which name Marinobacter halodurans sp. nov. is proposed, with the type strain YJ-S3-2T (=KACC 19883T=KCTC 62937T=JCM 33109T).