Lab Scale Extracted Conditions of Polyphenols from Thinned Peach Fruit Have Antioxidant, Hypoglycemic, and Hypolipidemic Properties

Thinned peach polyphenols (TPPs) were extracted by ultrasonic disruption and purified using macroporous resin. Optimized extraction conditions resulted in a TPPs yield of 1.59 ± 0.02 mg GAE/g FW, and optimized purification conditions resulted in a purity of 43.86% with NKA-9 resin. TPPs composition was analyzed by UPLC-ESI-QTOF-MS/MS; chlorogenic acid, catechin, and neochlorogenic acid were the most abundant compounds in thinned peaches. Purified TPPs exhibited scavenging activity on DPPH, ABTS, hydroxyl radical, and FRAP. TPPs inhibited α-amylase and α-glucosidase by competitive and noncompetitive reversible inhibition, respectively. TPPs also exhibited a higher binding capacity for bile acids than cholestyramine. In summary, TPPs from thinned peaches are potentially valuable because of their high antioxidant, hypoglycemic, and hypolipidemic capacities, and present a new incentive for the comprehensive utilization of thinned peach fruit.

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Simultaneous Determination of 5 Active Components of Periploca forrestii Schltr Extract in Rat Plasma by UPLC-MS and Its Application to Pharmacokinetic Studies in Normal and Adjuvant-Induced Arthritis Model Rats

Natural Product Communications ◽

10.1177/1934578x20981435 ◽

2020 ◽

Vol 15 (12) ◽

pp. 1934578X2098143

Author(s):

Hui Liu ◽

Hao Chen ◽

Xiaoli Qin ◽

Xue Ma ◽

Zipeng Gong ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Chlorogenic Acid ◽

Intravenous Administration ◽

Area Under The Curve ◽

Pharmacokinetic Studies ◽

Active Components ◽

Arthritis Model ◽

Adjuvant Induced Arthritis ◽

Neochlorogenic Acid

Periploca forrestii Schltr ( P. forrestii) is a herb used in traditional Chinese medicine for its anti-rheumatoid arthritis effect. The aim of this study was to compare the pharmacokinetic properties of the 5 active components of this plant: neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid C, and periplocin between normal rats and adjuvant-induced arthritis model rats. After the intravenous administration (177.78 mg/kg) of P. forrestii extract, samples were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry. Compared with normal rats, the area under the curve [(AUC)(0-t), AUC(0-∞)], mean residence time [(MRT)(0-t), MRT(0-∞)] of neochlorogenic acid-treated rats decreased significantly, and drug clearance (CL) and apparent volume of distribution (V) increased significantly; the V of chlorogenic acid-treated rats decreased significantly, and MRT(0-t) significantly increased; the AUC(0-t) and AUC(0-∞) of cryptochlorogenic acid-treated rats decreased significantly, and CL and V increased significantly; the AUC(0-t) and MRT(0-t) of isochlorogenic acid C-treated rats decreased significantly, and V increased significantly; the AUC(0-t) and AUC(0-∞) of periplocin-treated rats increased significantly, and MRT(0-t), MRT(0-∞), CL, and V decreased significantly in model rats. The disease condition of rheumatoid arthritis in rats had a significant effect on the in vivo pharmacokinetics of P. forrestii after the intravenous administration.

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Spectrophotometric studies on the interaction between chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid and lysozyme

Journal of Chinese Pharmaceutical Sciences ◽

10.5246/jcps.2014.08.070 ◽

2014 ◽

Vol 23 (8) ◽

Cited By ~ 1

Author(s):

Yuexiang Lan ◽

Hong Wang

Keyword(s):

Chlorogenic Acid ◽

Neochlorogenic Acid

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Increase in the hydroxyl radical-scavenging activity of Panax ginseng and ginsenosides by heat-processing

Drug Discoveries & Therapeutics ◽

10.5582/ddt.2018.01010 ◽

2018 ◽

Vol 12 (3) ◽

pp. 114-121 ◽

Cited By ~ 1

Author(s):

Chan Hum Park ◽

Jae Sue Choi ◽

Takako Yokozawa

Keyword(s):

Hydroxyl Radical ◽

Panax Ginseng ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Heat Processing ◽

Scavenging Activity ◽

Hydroxyl Radical Scavenging Activity

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Structure and Function of Mung Bean Protein-Derived Iron-Binding Antioxidant Peptides

Foods ◽

10.3390/foods9101406 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1406

Author(s):

Siriporn Chunkao ◽

Wirote Youravong ◽

Chutha T. Yupanqui ◽

Adeola M. Alashi ◽

Rotimi E. Aluko

Keyword(s):

Amino Acid ◽

Mung Bean ◽

Binding Capacity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Iron Binding ◽

Rp Hplc ◽

Iron Binding Capacity ◽

Bean Protein

An iron-binding mung bean protein hydrolysate (MBPH) was prepared using a continuous enzymatic membrane reactor followed by peptide separation on anion-exchange (AEC) and reverse-phase HPLC (RP-HPLC) columns. Amino acid sequences of peptides present in the RP-HPLC fraction with the strongest iron-binding capacity were identified using mass spectrometry, and ten peptides of 5–8 amino acids synthesized for antioxidant characterization. Five fractions (AF1– AF5) with higher iron-binding capacity (88.86 ± 6.43 to 153.59 ± 2.18 mg/g peptide) when compared to the MBPH (36.81 ± 0.93 mg/g peptide) were obtained from AEC. PAIDL had the significantly (p < 0.05) highest iron-binding capacity, but LLLLG and LLGIL showed the strongest metal chelating activity. However, PAIDL (46.63%) and LLGIL (81.27%) had significantly (p < 0.05) better DPPH radical scavenging activity than the other peptides. PAIDL and LLGIL were also the most effective (p < 0.05) hydroxyl radical neutralizers with an effective concentration that scavenged 50% (EC50) values of 0.09 and 0.37 mM, respectively. PAIDL and AIVIL showed the lowest EC50 values of 0.07 mM each for superoxide radical scavenging activity. We conclude that short chain length in combination with leucine as the C-terminal amino acid residue contributed to the strong antioxidant properties of peptides in this study.

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