scholarly journals A Comparison Between Two Assays for Measuring Seminal Oxidative Stress and their Relationship with Sperm DNA Fragmentation and Semen Parameters

Genes ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 236 ◽  
Author(s):  
Sheryl Homa ◽  
Anna Vassiliou ◽  
Jesse Stone ◽  
Aideen Killeen ◽  
Andrew Dawkins ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Dna Fragmentation ◽  
Recurrent Miscarriage ◽  
Reactive Oxygen ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Oxygen Species ◽  
Sperm Dna

Oxidative stress (OS) is a significant cause of DNA fragmentation and is associated with poor embryo development and recurrent miscarriage. The aim of this study was to compare two different methods for assessing seminal OS and their ability to predict sperm DNA fragmentation and abnormal semen parameters. Semen samples were collected from 520 men attending for routine diagnostic testing following informed consent. Oxidative stress was assessed using either a chemiluminescence assay to measure reactive oxygen species (ROS) or an electrochemical assay to measure oxidation reduction potential (sORP). Sperm DNA fragmentation (DFI) and sperm with immature chromatin (HDS) were assessed using sperm chromatin structure assay (SCSA). Semen analysis was performed according to WHO 2010 guidelines. Reactive oxygen species sORP and DFI are negatively correlated with sperm motility (p = 0.0012, 0.0002, <0.0001 respectively) and vitality (p < 0.0001, 0.019, <0.0001 respectively). The correlation was stronger for sORP than ROS. Reactive oxygen species (p < 0.0001), sORP (p < 0.0001), DFI (p < 0.0089) and HDS (p < 0.0001) were significantly elevated in samples with abnormal semen parameters, compared to those with normal parameters. Samples with polymorphonuclear leukocytes (PMN) have excessive ROS levels compared to those without (p < 0.0001), but sORP and DFI in this group are not significantly increased. DNA fragmentation was significantly elevated in samples with OS measured by ROS (p = 0.0052) or sORP (p = 0.004). The results demonstrate the multi-dimensional nature of oxidative stress and that neither assay can be used alone in the diagnosis of OS, especially in cases of leukocytospermia.

scholarly journals Reactive oxygen species and sperm DNA fragmentation

2017 ◽  
Vol 6 (S4) ◽  
pp. S695-S696 ◽  
Author(s):  
Ashok Agarwal ◽  
Chak-Lam Cho ◽  
Sandro C. Esteves ◽  
Ahmad Majzoub
Keyword(s):  
Reactive Oxygen Species ◽  
Dna Fragmentation ◽  
Reactive Oxygen ◽  
Sperm Dna Fragmentation ◽  
Oxygen Species ◽  
Sperm Dna

Semen characteristics and sperm DNA fragmentation in infertile men with low and high levels of seminal reactive oxygen species

2010 ◽  
Vol 94 (6) ◽  
pp. 2141-2146 ◽  
Author(s):  
Reda Mahfouz ◽  
Rakesh Sharma ◽  
Aparna Thiyagarajan ◽  
Vaishali Kale ◽  
Sajal Gupta ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Dna Fragmentation ◽  
Reactive Oxygen ◽  
Sperm Dna Fragmentation ◽  
Oxygen Species ◽  
Infertile Men ◽  
Sperm Dna ◽  
Semen Characteristics

INFLUENCE OF HYPERGLYCEMIA ON OXIDATIVE STRESS AND DNAase-MEDIATED GENOMIC FRAGMENTATION COUPLED WITH APOPTOTIC AND NECROTIC CELL DEATHS IN KIDNEY

2011 ◽  
Vol 1 ◽  
pp. 129-143
Author(s):  
Mohammed saleem ◽  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Dna Fragmentation ◽  
Potent Inhibitor ◽  
Reactive Oxygen ◽  
Prostaglandin Synthesis ◽  
Oxygen Species ◽  
Necrotic Cell ◽  
Analgesic Agent

Diclofenac (DCLF) is a potent inhibitor of prostaglandin synthesis and an established antipyretic and analgesic agent. It also has a nephrotoxic profile caused by generation of reactive oxygen species and enhanced apoptotic DNA fragmentation. The specific goals of this investigation were to determine

313 ASSESSMENT OF SPERM DNA FRAGMENTATION IN CANINE EJACULATES USING THE Sperm-Halomax® KIT: PRELIMINARY RESULTS

2010 ◽  
Vol 22 (1) ◽  
pp. 312 ◽  
Author(s):  
M. Hidalgo ◽  
M. R. Murabito ◽  
M. J. Gálvez ◽  
S. Demyda ◽  
L. J. De Luca ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Commercial Kit ◽  
Sperm Dna Fragmentation Index ◽  
Dna Fragmentation Index

Recently, a new procedure for the analysis of sperm DNA fragmentation has been developed for humans and different mammalian species, using a commercial kit based on the sperm chromatin dispersion (SCD) test; however, a descriptive study in canine semen has not been performed. The aim of this work was to assess the sperm DNA fragmentation in canine ejaculates using the SCD test and 2 different staining techniques. For this purpose, ejaculates were collectedby digital manipulation from4 healthy dogs of different breeds (1 German Pointer, 2 Spanish Greyhounds, and 1 Crossbreed). After collection, the sperm-rich fraction of the ejaculates from 3 dogs were pooled each time (n = 4) and then extended in Dulbecco’s phosphate buffered saline. All the pooled semen samples presented physiological values concerning routine semen parameters (motility, morphology, and sperm concentration). The sperm DNA fragmentation was assessed using the Sperm-Halomax® commercial kit specifically developed for canine semen (Halotech DNA SL, Madrid, Spain). Two semen aliquots of the diluted pooled semen samples were processed on each pre-treated slide provided in the kit following the manufacturer’s instructions. The last step was the staining technique. We stained each slide with 2 different staining procedures. The first half of the slide was stained with propidium iodide (Sigma-Aldrich, St. Louis, MO, USA) mixed in a proportion 1 : 1 with an antifading solution. The second half of the slide was stained for 15 min in Wright solution (1.01383.0500, Merck, Whitehouse Station, NJ, USA) 1 :1 in Phosphate Buffer pH 6.88 (1.07294.1000, Merck). The stained slides were observed using fluorescence and light microscopy, respectively. Five hundred sperm per slide were counted. Spermatozoa with fragmented DNA showed a large and spotty halo of chromatin dispersion. Unfragmented sperm only showed a small and compact halo. Statistical analyses were performed using the Statistical Package for Social Science version 12.0 (SPSS Inc., Chicago, IL, USA). The sperm DNA fragmentation index was compared between Wright and fluorescence staining methods by ANOVA. Results were expressed as mean ± standard error of the mean. The first report of the sperm DNA fragmentation index in canine ejaculates was 2.26 ± 0.53% for Wright staining and 1.99 ± 0.10% for fluorescence technique. No differences were found between staining procedures. In conclusion, it was possible to assess the sperm DNA fragmentation of canine ejaculates using 2 different staining procedures, expecting that continuous research could be useful in defining the role of DNA fragmentation SCD test in canine semen evaluation and cryopreservation.

Relation between Traditional Semen Parameters, Sperm DNA Fragmentation, and Unexplained Recurrent Miscarriage: A Systematic Review and Meta-analysis

2021 ◽  
Author(s):  
Yanpeng Dai ◽  
Junjie Liu ◽  
Enwu Yuan ◽  
Ying Shi ◽  
Linlin Zhang
Keyword(s):  
Dna Fragmentation ◽  
Fixed Effects ◽  
Recurrent Miscarriage ◽  
Meta Analysis ◽  
Semen Parameters ◽  
Cochrane Library ◽  
Sperm Dna Fragmentation ◽  
Progressive Motility ◽  
Sperm Dna ◽  
Unexplained Recurrent Miscarriage

Abstract Background: Several studies have explored the relation between traditional semen parameters, sperm DNA fragmentation (SDF), and unexplained recurrent miscarriage (RM), but these findings remain controversial. Hence, we conducted this meta-analysis to explore the relation between traditional semen parameters, SDF, and unexplained RM. Methods: Multiple databases including PubMed, Google Scholar, MEDLINE, EMBASE, Cochrane Library, Web of Science databases, and China National Knowledge Infrastructure (CNKI) were searched to identify relevant publications. From the eligible publications, data were extracted independently by two researchers. The heterogeneity between publications was calculated using the I2 statistics and Cochran’s Q test. Statistical analyses were conducted using Stata/SE 12.0 (StataCorp, College Station, Texas, USA). Based on heterogeneity assessment, random- or fixed-effects models were selected to calculate the weighted mean differences (WMDs) and their corresponding 95% confidence intervals (CIs). To estimate the stability of the pooled results, a sensitivity analysis was conducted by excluding each study. To estimate the possible publication bias, Egger’s regression test and Begg’s funnel plot were used. Results: A total of 280 publications were produced using the search strategy. According to the inclusion/exclusion criteria, 19 publications were eligible. A total of 1182 couples with unexplained RM and 1231 couples without RM were included in this meta-analysis to assess the relation between traditional semen parameters, SDF, and unexplained RM. Our results showed that couples with unexplained RM had significantly increased levels of SDF (WMD=8.77, 95% CI=4.03 to 13.51, P<0.001) and significantly decreased levels of progressive motility (WMD=-4.75, 95% CI=-8.35 to -1.15, P<0.05) and total motility (WMD=-10.30, 95% CI=-15.03 to -5.57, P<0.05) than those of couples without RM, but not significantly different in volume (WMD=-0.12, 95% CI=-0.32 to 0.08, P>0.05), sperm concentration (WMD=-2.28, 95% CI=-4.58 to 0.02, P>0.05) and total sperm count (WMD=-10.73, 95% CI=-22.11 to 0.66, P>0.05) between couples with and without RM.Conclusion: Couples with unexplained RM had significantly increased levels of SDF and significantly decreased levels of progressive motility and total motility than those of couples without RM. SDF assay may be considered as part of the evaluation of couples with unexplained RM.

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