Overexpression of Lilium formosanum MADS-box (LFMADS) Causing Floral Defects While Promoting Flowering in Arabidopsis thaliana, Whereas Only Affecting Floral Transition Time in Nicotiana tabacum

The Formosa lily (Lilium formosanum) is one of the most common horticultural species in Taiwan. To explore gene regulation involved in this species, we used transcriptome analysis to generate PH-FB (mixed floral buds) and PH-LF (mature leaves) datasets. Combination of the PH-FB and PH-LF constructed a de novo assembly of the ALL dataset, including 18,041 contigs and 23,807 unigenes by Nr, GO, COG, and KEGG databases. The differential gene expression (DGE) analysis revealed 9937 genes were upregulated while 10,383 genes were downregulated in the developing floral buds compared to mature leaves. Seven putative genes (LFMADS1 to 7) encoding floral organ identity proteins were selected for further analysis. LFMADS1-6 genes were specifically expressed in the floral organ, while LFMADS7 in the floral buds and mature leaves. Phylogenetic analysis revealed that LFMADS1-3 is classified into B-class, LFMADS4 into C-class, LFMADS5 into D-class, and LFMADS6-7 into E-class, respectively. LFMADS-GFP fusion proteins appeared to localize in the nucleus, supporting their roles as transcription factors (TFs). Overexpression of the LFMADS2, LFMADS4, and LFMADS6 genes in Arabidopsis resulted in early flowering and floral defect, however, only early flowering in transgenic tobacco was observed. Highly expressed floral integrator genes, including AtFT, AtLFY, and AtFUL in transgenic Arabidopsis and NtFUL and NtSOC1 in transgenic tobacco, resulted in early flowering phenotype through qRT-PCR analysis. Yeast two-hybrid analysis suggested that LFMADSs may form higher order complexes with the B-, C-, D, and/or E-class proteins to determine the floral organ identity. Furthermore, E-class LFMADS proteins may function as a glue to mediate and strengthen the protein-protein interactions. Therefore, our de novo datasets would provide information for investigating other differentially expressed candidate transcripts. In addition, functional conservation of LFMADSs appears to be vital in floral transition and floral organ identity.

Download Full-text

MIR172d Is Required for Floral Organ Identity and Number in Tomato

International Journal of Molecular Sciences ◽

10.3390/ijms22094659 ◽

2021 ◽

Vol 22 (9) ◽

pp. 4659

Author(s):

Wanping Lin ◽

Suresh Kumar Gupta ◽

Tzahi Arazi ◽

Ben Spitzer-Rimon

Keyword(s):

Transcription Factors ◽

Transcriptional Repression ◽

De Novo ◽

Floral Organ ◽

Dependent Manner ◽

Loss Of Function ◽

Floral Organ Identity ◽

Organ Identity ◽

Unique Species ◽

Dose Dependent

MicroRNA172 (miR172) functions as a central regulator of flowering time and flower development by post-transcriptional repression of APETALA2-LIKE transcription factors. In the model crop Solanum lycopersicum (tomato), the miR172 family is still poorly annotated and information about the functions of specific members is lacking. Here, de-novo prediction of tomato miR172 coding loci identified seven genes (SlMIR172a-g), that code for four unique species of miR172 (sly-miR172). During reproductive development, sly-miR172s are differentially expressed, with sly-miR172c and sly-miR172d being the most abundant members in developing flowers, and are predicted to guide the cleavage of eight APETALA2-LIKE transcription factors. By CRISPR-Cas9 co-targeting of SlMIR172c and SlMIR172d we have generated a battery of loss-of-function and hypomorphic mutants (slmir172c-dCR). The slmir172c-dCR plants developed normal shoot but their flowers displayed graded floral organ abnormalities. Whereas slmir172cCR loss-of-function caused only a slight greening of petals and stamens, hypomorphic and loss-of-function slmir172dCR alleles were associated with the conversion of petals and stamens to sepaloids, which were produced in excess. Interestingly, the degrees of floral organ identity alteration and proliferation were directly correlated with the reduction in sly-miR172d activity. These results suggest that sly-miR172d regulates in a dose-dependent manner floral organ identity and number, likely by negatively regulating its APETALA2-like targets.

Download Full-text

Faculty Opinions recommendation of A conserved microRNA module exerts homeotic control over Petunia hybrida and Antirrhinum majus floral organ identity.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1089029.543158 ◽

2007 ◽

Author(s):

Rüdiger Simon

Keyword(s):

Petunia Hybrida ◽

Floral Organ ◽

Antirrhinum Majus ◽

Floral Organ Identity ◽

Organ Identity

Download Full-text

Conserved C-Terminal Motifs of the Arabidopsis Proteins APETALA3 and PISTILLATA Are Dispensable for Floral Organ Identity Function

PLANT PHYSIOLOGY ◽

10.1104/pp.107.105346 ◽

2007 ◽

Vol 145 (4) ◽

pp. 1495-1505 ◽

Cited By ~ 41

Author(s):

Eileen Piwarzyk ◽

Yingzhen Yang ◽

Thomas Jack

Keyword(s):

Floral Organ ◽

Floral Organ Identity ◽

Identity Function ◽

Organ Identity ◽

Arabidopsis Proteins

Download Full-text

The Origin of Floral Organ Identity Quartets

The Plant Cell ◽

10.1105/tpc.16.00366 ◽

2017 ◽

Vol 29 (2) ◽

pp. 229-242 ◽

Cited By ~ 14

Author(s):

Philip Ruelens ◽

Zhicheng Zhang ◽

Hilda van Mourik ◽

Steven Maere ◽

Kerstin Kaufmann ◽

...

Keyword(s):

Floral Organ ◽

Floral Organ Identity ◽

Organ Identity

Download Full-text

Alteration of floral organ identity in rice through ectopic expression of OsMADS16

Planta ◽

10.1007/s00425-003-1066-8 ◽

2003 ◽

Vol 217 (6) ◽

pp. 904-911 ◽

Cited By ~ 51

Author(s):

Sichul Lee ◽

Jong-Seong Jeon ◽

Kyungsook An ◽

Yong-Hwan Moon ◽

Sanghee Lee ◽

...

Keyword(s):

Ectopic Expression ◽

Floral Organ ◽

Floral Organ Identity ◽

Organ Identity

Download Full-text

Functional conservation and divergence of five AP1/FUL-like genes in Marigold (Tagetes erecta)

10.21203/rs.3.rs-88138/v1 ◽

2020 ◽

Author(s):

Chunling Zhang ◽

Yalin Sun ◽

Ludan Wei ◽

Wenjing Wang ◽

Hang Li ◽

...

Keyword(s):

Profile Analysis ◽

Floral Organ ◽

Tagetes Erecta ◽

Functional Conservation ◽

Floral Buds ◽

Expression Profile Analysis ◽

Petal Identity ◽

Organ Identity ◽

Insight Into

Abstract Background: Members of AP1/FUL subfamily genes play an essential role in the regulation of floral meristem transition, floral organ identity, and fruit ripping. At present, there have been insufficient studies to explain the function of the AP1/FUL-like subfamily genes in Asteraceae. Results: Here, we cloned two euAP1 clade genes TeAP1-1 and TeAP1-2, and three euFUL clade genes TeFUL1, TeFUL2, and TeFUL3 from marigold (Tagetes erecta). Expression profile analysis demonstrated that TeAP1-1 and TeAP1-2 were mainly expressed in receptacles, sepals, petals, and ovules. TeFUL1 and TeFUL3 were expressed in floral buds, stems and leaves as well as in productive tissues, while TeFUL2 was mainly expressed in floral buds and vegetative tissues. Transgenic Arabidopsis lines showed that overexpression TeAP1-2 or TeFUL2 resulted in early flowering, implying that these two genes might regulate the floral transition. Yeast two-hybrid analysis indicated that TeAP1/FUL proteins only interacted with TeSEP proteins to form heterodimers, and that TeFUL2 could also form a homodimer.Conclusion: In general, TeAP1-1 and TeAP1-2 might play a conserved role in regulating sepal and petal identity, just like the role of MADS-box class A genes, while TeFUL genes might display divergent functions. This study provides an insight into molecular mechanism of AP1/FUL-like genes in Asteraceae species.

Download Full-text